Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS07_RS07365 Genome accession   NZ_CP121837
Coordinates   1543553..1544662 (+) Length   369 a.a.
NCBI ID   WP_101530224.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18263     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1538553..1549662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS07_RS07335 - 1539454..1539873 (-) 420 WP_113740088.1 DUF4426 domain-containing protein -
  QBS07_RS07340 yggU 1539917..1540219 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS07_RS07345 - 1540219..1540770 (-) 552 WP_005340343.1 YggT family protein -
  QBS07_RS07350 proC 1540782..1541606 (-) 825 WP_042053316.1 pyrroline-5-carboxylate reductase -
  QBS07_RS07355 - 1541749..1542450 (-) 702 WP_279488828.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS07_RS07360 pilT 1542490..1543524 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS07_RS07365 pilU 1543553..1544662 (+) 1110 WP_101530224.1 type IVa pilus ATPase TapU Machinery gene
  QBS07_RS07370 yaaA 1544710..1545483 (+) 774 WP_064338818.1 peroxide stress protein YaaA -
  QBS07_RS07375 - 1545678..1546103 (+) 426 WP_279488829.1 hypothetical protein -
  QBS07_RS07380 - 1546465..1546956 (-) 492 WP_279488830.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS07_RS07385 arfB 1546987..1547403 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS07_RS07390 glnK 1547605..1547943 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS07_RS07395 - 1548202..1549209 (+) 1008 WP_279489135.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41104.17 Da        Isoelectric Point: 6.5043

>NTDB_id=814526 QBS07_RS07365 WP_101530224.1 1543553..1544662(+) (pilU) [Aeromonas veronii strain ANYA 18263]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEVLDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814526 QBS07_RS07365 WP_101530224.1 1543553..1544662(+) (pilU) [Aeromonas veronii strain ANYA 18263]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCTCTGGGGGGGGAGGTGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGCCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTAAAAAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACAGGCCACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCTATCGTCGCCCAGCA
GTTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACAGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36