Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS10_RS08135 Genome accession   NZ_CP121802
Coordinates   1691890..1692924 (-) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BOND 14504     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686890..1697924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS10_RS08115 - 1687708..1688418 (-) 711 WP_017765780.1 methyltransferase -
  QBS10_RS08120 srmB 1688547..1689770 (+) 1224 WP_279452237.1 ATP-dependent RNA helicase SrmB -
  QBS10_RS08125 yaaA 1689947..1690720 (-) 774 WP_017783834.1 peroxide stress protein YaaA -
  QBS10_RS08130 pilU 1690752..1691861 (-) 1110 WP_019838905.1 type IVa pilus ATPase TapU Machinery gene
  QBS10_RS08135 pilT 1691890..1692924 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  QBS10_RS08140 - 1692964..1693665 (+) 702 WP_279452238.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS10_RS08145 proC 1693726..1694550 (+) 825 WP_123246602.1 pyrroline-5-carboxylate reductase -
  QBS10_RS08150 - 1694591..1695142 (+) 552 WP_005305000.1 YggT family protein -
  QBS10_RS08155 yggU 1695142..1695441 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  QBS10_RS08160 - 1695460..1695879 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  QBS10_RS08165 - 1695943..1696254 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=814368 QBS10_RS08135 WP_005305010.1 1691890..1692924(-) (pilT) [Aeromonas dhakensis strain BOND 14504]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814368 QBS10_RS08135 WP_005305010.1 1691890..1692924(-) (pilT) [Aeromonas dhakensis strain BOND 14504]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAGCACCGGGAAGTACACGCCCTCATCTATGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCGAACCTGGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACAGTGCTGAGCCTGGA
AGATCTGGATGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCGCGCGGCCTGGTGCTGGTGACCGGTCCGACCGGTT
CGGGCAAGTCCACTACCTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTGCACCGCGACACCAAGAGCTTCAGCAA
CGCACTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGTGAAATGCGCGACCTGGAGACCATTCGCCTCG
CCATGACCGCGGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGGATC
ATCGACGTCTTTCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAATCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGGATCCCCGCCGTGCGTAACCTCATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGCGGCATGGTGGCGGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407