Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS10_RS08130 Genome accession   NZ_CP121802
Coordinates   1690752..1691861 (-) Length   369 a.a.
NCBI ID   WP_019838905.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BOND 14504     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1685752..1696861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS10_RS08110 brnQ 1686209..1687471 (-) 1263 WP_279452720.1 branched-chain amino acid transport system II carrier protein -
  QBS10_RS08115 - 1687708..1688418 (-) 711 WP_017765780.1 methyltransferase -
  QBS10_RS08120 srmB 1688547..1689770 (+) 1224 WP_279452237.1 ATP-dependent RNA helicase SrmB -
  QBS10_RS08125 yaaA 1689947..1690720 (-) 774 WP_017783834.1 peroxide stress protein YaaA -
  QBS10_RS08130 pilU 1690752..1691861 (-) 1110 WP_019838905.1 type IVa pilus ATPase TapU Machinery gene
  QBS10_RS08135 pilT 1691890..1692924 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  QBS10_RS08140 - 1692964..1693665 (+) 702 WP_279452238.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS10_RS08145 proC 1693726..1694550 (+) 825 WP_123246602.1 pyrroline-5-carboxylate reductase -
  QBS10_RS08150 - 1694591..1695142 (+) 552 WP_005305000.1 YggT family protein -
  QBS10_RS08155 yggU 1695142..1695441 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  QBS10_RS08160 - 1695460..1695879 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  QBS10_RS08165 - 1695943..1696254 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41139.26 Da        Isoelectric Point: 6.4437

>NTDB_id=814367 QBS10_RS08130 WP_019838905.1 1690752..1691861(-) (pilU) [Aeromonas dhakensis strain BOND 14504]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814367 QBS10_RS08130 WP_019838905.1 1690752..1691861(-) (pilU) [Aeromonas dhakensis strain BOND 14504]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCGCCACTCGACAAGGCAAGGGCACTGGCACTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTACTGCGCCGCATTGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATCGCCCTCGCCAAGCGCGGGCTGGTACTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACGGAGTCGTTCGACGT
GGCGCTGAAGAGCTCACTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAATTTGCCGAGACCGGTCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTGCCGCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCATCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTTACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363