Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS29_RS10020 Genome accession   NZ_CP121794
Coordinates   1446436..1447470 (-) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas caviae strain FIONA 16800     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1441436..1452470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS29_RS10000 - 1442251..1442961 (-) 711 WP_069652226.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  QBS29_RS10005 srmB 1443089..1444309 (+) 1221 WP_266109759.1 ATP-dependent RNA helicase SrmB -
  QBS29_RS10010 yaaA 1444497..1445270 (-) 774 WP_167825797.1 peroxide stress protein YaaA -
  QBS29_RS10015 pilU 1445303..1446412 (-) 1110 WP_010673049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QBS29_RS10020 pilT 1446436..1447470 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  QBS29_RS10025 - 1447510..1448211 (+) 702 WP_039039960.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS29_RS10030 proC 1448335..1449159 (+) 825 WP_266109760.1 pyrroline-5-carboxylate reductase -
  QBS29_RS10035 - 1449183..1449734 (+) 552 WP_042014922.1 YggT family protein -
  QBS29_RS10040 yggU 1449734..1450033 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  QBS29_RS10045 - 1450052..1450471 (+) 420 WP_202043103.1 DUF4426 domain-containing protein -
  QBS29_RS10050 - 1450547..1451377 (-) 831 WP_043153034.1 CPBP family intramembrane glutamic endopeptidase -
  QBS29_RS10055 - 1451539..1451829 (-) 291 WP_039039963.1 hypothetical protein -
  QBS29_RS10060 - 1451879..1452253 (-) 375 WP_039039964.1 YacL family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=814229 QBS29_RS10020 WP_010673050.1 1446436..1447470(-) (pilT) [Aeromonas caviae strain FIONA 16800]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814229 QBS29_RS10020 WP_010673050.1 1446436..1447470(-) (pilT) [Aeromonas caviae strain FIONA 16800]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCTCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTACTGGTGACTGGGCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTACGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGCCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAACTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTCGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407