Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS29_RS10015 Genome accession   NZ_CP121794
Coordinates   1445303..1446412 (-) Length   369 a.a.
NCBI ID   WP_010673049.1    Uniprot ID   A0AAJ6CRB8
Organism   Aeromonas caviae strain FIONA 16800     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1440303..1451412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS29_RS09990 - 1440436..1440663 (+) 228 WP_039039956.1 hypothetical protein -
  QBS29_RS09995 brnQ 1440753..1442060 (-) 1308 WP_043153135.1 branched-chain amino acid transport system II carrier protein -
  QBS29_RS10000 - 1442251..1442961 (-) 711 WP_069652226.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  QBS29_RS10005 srmB 1443089..1444309 (+) 1221 WP_266109759.1 ATP-dependent RNA helicase SrmB -
  QBS29_RS10010 yaaA 1444497..1445270 (-) 774 WP_167825797.1 peroxide stress protein YaaA -
  QBS29_RS10015 pilU 1445303..1446412 (-) 1110 WP_010673049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QBS29_RS10020 pilT 1446436..1447470 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  QBS29_RS10025 - 1447510..1448211 (+) 702 WP_039039960.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS29_RS10030 proC 1448335..1449159 (+) 825 WP_266109760.1 pyrroline-5-carboxylate reductase -
  QBS29_RS10035 - 1449183..1449734 (+) 552 WP_042014922.1 YggT family protein -
  QBS29_RS10040 yggU 1449734..1450033 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  QBS29_RS10045 - 1450052..1450471 (+) 420 WP_202043103.1 DUF4426 domain-containing protein -
  QBS29_RS10050 - 1450547..1451377 (-) 831 WP_043153034.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41000.18 Da        Isoelectric Point: 6.4409

>NTDB_id=814228 QBS29_RS10015 WP_010673049.1 1445303..1446412(-) (pilU) [Aeromonas caviae strain FIONA 16800]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLRPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814228 QBS29_RS10015 WP_010673049.1 1445303..1446412(-) (pilU) [Aeromonas caviae strain FIONA 16800]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCAGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCCCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAATTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTATCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TTCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCA
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.56

91.057

0.388
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379