Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MARGE09_RS12405 Genome accession   NZ_AP023086
Coordinates   3095886..3096920 (+) Length   344 a.a.
NCBI ID   WP_236982353.1    Uniprot ID   -
Organism   Marinagarivorans cellulosilyticus strain GE09     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3090886..3101920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARGE09_RS12390 (MARGE09_P2370) - 3093420..3093995 (-) 576 WP_236982347.1 YggT family protein -
  MARGE09_RS12395 (MARGE09_P2371) proC 3094035..3094844 (-) 810 WP_236982349.1 pyrroline-5-carboxylate reductase -
  MARGE09_RS12400 (MARGE09_P2372) - 3094911..3095588 (-) 678 WP_236982351.1 YggS family pyridoxal phosphate-dependent enzyme -
  MARGE09_RS12405 (MARGE09_P2373) pilT 3095886..3096920 (+) 1035 WP_236982353.1 type IV pilus twitching motility protein PilT Machinery gene
  MARGE09_RS12410 (MARGE09_P2374) pilU 3097010..3098152 (+) 1143 WP_236982355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MARGE09_RS12415 (MARGE09_P2375) - 3098219..3100063 (-) 1845 WP_236982357.1 sugar transferase -
  MARGE09_RS12420 (MARGE09_P2376) - 3100073..3101140 (-) 1068 WP_236982359.1 glycosyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38258.05 Da        Isoelectric Point: 6.5771

>NTDB_id=81250 MARGE09_RS12405 WP_236982353.1 3095886..3096920(+) (pilT) [Marinagarivorans cellulosilyticus strain GE09]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKSVHALIYEIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEELGMGQVFRDICSVPRGLVLVTGPTGSGKSTTLAAMVDFINESKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPANEKAMVRSMLSESLQAVISQTLMKRVGGGRIAAHEIMRGTPAIRNLIREDKVAQMYSSIQTGSAVGMQTMDQCL
ADLVERRIISRDIAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=81250 MARGE09_RS12405 WP_236982353.1 3095886..3096920(+) (pilT) [Marinagarivorans cellulosilyticus strain GE09]
ATGGATATTACTGAACTGTTGGCGTTTTCTGCCAAGCAGGGTGCTTCCGACTTACACCTCTCGGCAGGCTTACCGCCAAT
GATTCGTGTCGATGGCGATGTGCGTCGCATTAACTTGCCGCCGATGGATCACAAATCGGTGCATGCGTTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAAGATTTCGAAGAGTTCCTAGAAACCGATTTCTCTTTCGAAGTGCCCGGCGTGGCGCGT
TTTCGTGTCAACGCTTTTAACCACAACCGCGGTGCCGGTGCGGTTTTCCGTACCATTCCATCGAAAGTACTGACGATGGA
AGAGCTCGGTATGGGGCAGGTGTTTCGTGATATTTGCTCTGTGCCACGGGGTCTAGTATTGGTAACAGGGCCTACTGGTT
CGGGTAAGTCTACAACGCTGGCAGCGATGGTCGACTTTATCAACGAAAGTAAGTACGAACACATCCTTACCATCGAAGAC
CCTATCGAATTCGTTCACGAGTCGAAAAAATGCTTGGTTAACCAGCGCGAAGTACACCGCGATACTCACGGCTTTGCTGA
AGCGCTTCGCTCGGCGCTGCGGGAAGATCCCGATATCATTCTTGTTGGTGAGATGCGAGACCTAGAAACCATTCGCTTGG
CGCTAACAGCTGCTGAAACGGGCCACTTAGTTTTCGGCACCTTGCACACTACCTCAGCTGCTAAAACCATCGACCGTATT
GTAGATGTATTCCCCGCTAACGAGAAAGCCATGGTGCGTTCGATGCTGTCGGAATCGTTACAGGCTGTAATTTCGCAAAC
CCTTATGAAGCGTGTTGGCGGCGGCCGTATTGCAGCACATGAAATTATGCGCGGTACGCCCGCGATCCGTAACTTGATTC
GTGAAGATAAAGTTGCACAAATGTATTCATCGATTCAAACGGGCTCGGCTGTGGGCATGCAGACGATGGATCAATGCTTG
GCAGACCTCGTCGAGCGTCGCATTATTAGTCGCGATATAGCGCGCGAAAAAGCGAAAATGCCAGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.372

100

0.884
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baumannii D1279779

81.686

100

0.817
  pilT Acinetobacter baumannii strain A118

81.686

100

0.817
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

73.964

98.256

0.727
  pilT Legionella pneumophila strain ERS1305867

73.964

98.256

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39
  pilU Vibrio cholerae strain A1552

40.923

94.477

0.387
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.186

0.384

Multiple sequence alignment