Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MARGE09_RS12410 Genome accession   NZ_AP023086
Coordinates   3097010..3098152 (+) Length   380 a.a.
NCBI ID   WP_236982355.1    Uniprot ID   A0AAN1WIE4
Organism   Marinagarivorans cellulosilyticus strain GE09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3092010..3103152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARGE09_RS12390 (MARGE09_P2370) - 3093420..3093995 (-) 576 WP_236982347.1 YggT family protein -
  MARGE09_RS12395 (MARGE09_P2371) proC 3094035..3094844 (-) 810 WP_236982349.1 pyrroline-5-carboxylate reductase -
  MARGE09_RS12400 (MARGE09_P2372) - 3094911..3095588 (-) 678 WP_236982351.1 YggS family pyridoxal phosphate-dependent enzyme -
  MARGE09_RS12405 (MARGE09_P2373) pilT 3095886..3096920 (+) 1035 WP_236982353.1 type IV pilus twitching motility protein PilT Machinery gene
  MARGE09_RS12410 (MARGE09_P2374) pilU 3097010..3098152 (+) 1143 WP_236982355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MARGE09_RS12415 (MARGE09_P2375) - 3098219..3100063 (-) 1845 WP_236982357.1 sugar transferase -
  MARGE09_RS12420 (MARGE09_P2376) - 3100073..3101140 (-) 1068 WP_236982359.1 glycosyltransferase -
  MARGE09_RS12425 (MARGE09_P2377) - 3101317..3102759 (-) 1443 WP_236982360.1 GumC family protein -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42454.65 Da        Isoelectric Point: 6.0212

>NTDB_id=81251 MARGE09_RS12410 WP_236982355.1 3097010..3098152(+) (pilU) [Marinagarivorans cellulosilyticus strain GE09]
MEIDRLLRLMVEKQASDLFITAGVPPSMKVNGKITPVTTTPLSPEKSRETVLSTMNEKQREEFVDKKELNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETKIPKVDELGLPEIIKDLAMTKRGLIIFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFVHQHQGCVITQREVGIDTESFDVALKNTLRQAPDVILIGEVRSRETMENAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHTQLWMDLSLNLKAIVAQQLIPTPDGNGRRACLEIMLNTPLAADTIRKGEVHELKELMKKSTELGMQTFDQA
LYDLYDAGEITYEDALAHADSANDLRLLIKLGSESDAEYLSNAADELSIQEDDTNRMRRF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=81251 MARGE09_RS12410 WP_236982355.1 3097010..3098152(+) (pilU) [Marinagarivorans cellulosilyticus strain GE09]
ATGGAAATTGATCGACTCCTCCGCTTAATGGTGGAAAAACAAGCGTCCGACTTATTTATTACTGCCGGTGTACCGCCATC
AATGAAGGTAAACGGTAAAATTACCCCAGTCACAACAACGCCTTTATCGCCCGAAAAGTCGCGTGAAACGGTGCTTAGCA
CCATGAACGAAAAACAGCGCGAAGAGTTCGTTGATAAAAAAGAGCTAAACTTTGCCATCAGCGCCCGTGGTGTTGGCCGT
TTCCGTGTGAGTGCTTTTTATCAGCGCAACCTTGCTGGTATGGTTTTGCGTCGCATTGAAACCAAAATTCCAAAGGTTGA
TGAATTGGGCTTGCCTGAAATCATTAAAGACCTAGCTATGACAAAGCGCGGCCTAATTATTTTTGTGGGCGCTACCGGTA
CGGGTAAATCCACATCGCTCGCGGCGATGATTGGGCACCGCAACCGCAACTCGAAAGGCCATATTATTTCCATTGAAGAT
CCTATTGAATTTGTTCACCAGCACCAAGGTTGCGTTATTACGCAGCGTGAGGTGGGCATTGATACTGAATCTTTTGATGT
AGCTTTGAAAAACACATTGCGTCAAGCACCCGATGTGATTTTGATTGGTGAGGTTCGTTCGCGCGAAACAATGGAAAACG
CCATTGCCTTTGCTGAAACCGGCCACTTATGTTTGTGTACACTGCACGCTAACAACGCTAACCAAGCATTGGATCGCATT
ATTCACTTTTTCCCCGCCGATCGTCATACGCAACTGTGGATGGATTTGTCGCTAAACTTAAAAGCCATTGTGGCGCAGCA
GTTAATTCCCACGCCCGACGGCAATGGTCGCCGCGCGTGTTTAGAAATTATGCTTAATACGCCATTAGCAGCAGATACTA
TCCGTAAGGGCGAAGTACACGAGCTGAAAGAGTTAATGAAAAAATCAACCGAGCTGGGCATGCAGACTTTTGACCAAGCG
TTATACGATTTATATGACGCCGGTGAAATCACCTACGAGGATGCATTGGCGCACGCTGACTCTGCCAACGATTTACGCCT
GCTTATTAAATTGGGCTCTGAATCGGATGCGGAGTACTTGTCGAATGCCGCCGATGAGTTGTCTATTCAGGAAGATGATA
CCAATCGGATGCGTAGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.579

100

0.766
  pilU Acinetobacter baylyi ADP1

66.667

93.947

0.626
  pilU Vibrio cholerae strain A1552

56.286

92.105

0.518
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

89.211

0.395
  pilT Legionella pneumophila strain Lp02

41.194

88.158

0.363
  pilT Legionella pneumophila strain ERS1305867

41.194

88.158

0.363
  pilT Acinetobacter baumannii strain A118

40.896

88.158

0.361
  pilT Pseudomonas aeruginosa PAK

40.896

88.158

0.361
  pilT Acinetobacter nosocomialis M2

40.896

88.158

0.361
  pilT Acinetobacter baumannii D1279779

40.896

88.158

0.361
  pilT Vibrio cholerae strain A1552

41.018

87.895

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

87.895

0.361

Multiple sequence alignment