Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Thiosp_RS21400 Genome accession   NZ_CP121473
Coordinates   4696072..4697127 (+) Length   351 a.a.
NCBI ID   WP_201062985.1    Uniprot ID   -
Organism   Thiorhodovibrio litoralis strain 06511     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4691072..4702127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiosp_RS21385 (Thiosp_04333) - 4692376..4693821 (+) 1446 WP_201062988.1 GTPase/DUF3482 domain-containing protein -
  Thiosp_RS21390 (Thiosp_04334) - 4693914..4694486 (-) 573 WP_207187954.1 DsrE family protein -
  Thiosp_RS21395 (Thiosp_04335) pilU 4694741..4696015 (+) 1275 WP_201062986.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiosp_RS21400 (Thiosp_04336) pilU 4696072..4697127 (+) 1056 WP_201062985.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiosp_RS21405 (Thiosp_04337) hisC 4697236..4698375 (-) 1140 WP_201062984.1 histidinol-phosphate transaminase -
  Thiosp_RS21410 (Thiosp_04338) hisD 4698405..4699709 (-) 1305 WP_201062983.1 histidinol dehydrogenase -
  Thiosp_RS21415 (Thiosp_04339) hisG 4699730..4700365 (-) 636 WP_201062982.1 ATP phosphoribosyltransferase -
  Thiosp_RS21420 (Thiosp_04340) murA 4700449..4701732 (-) 1284 WP_201062981.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39274.43 Da        Isoelectric Point: 6.6940

>NTDB_id=812350 Thiosp_RS21400 WP_201062985.1 4696072..4697127(+) (pilU) [Thiorhodovibrio litoralis strain 06511]
MDITPYLKLMVQKHASDLFFTAGTQVKIKIDGVIQSIGDTVFDTNMCAAAIHSILTREQIEFFETHREIDFGIGLTGQGR
FRVNAFHQRGTPAMVIRYLTSKIPTIEELGLPEILKRLILHRRGIILMVGATGSGKSTTLAAMIDHRNISRPGHILTIED
PIEYLHGHKKSIVNQRELGIDTMDYATALKSALREAPDVILIGEIRTRETMEAAIELSGTGHLAISTLHANNAYQTMERI
INMFPKEMHRTLFMDLSLNLRAIISQRLVRTVEGKRVAAIEILMNTPHISDLIREGRVDEIRQAMAESKEEGMTTFDDAL
LKLYRDGLISLEECLANSDSAANLEARINFG

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=812350 Thiosp_RS21400 WP_201062985.1 4696072..4697127(+) (pilU) [Thiorhodovibrio litoralis strain 06511]
ATGGACATCACGCCCTACCTGAAACTGATGGTGCAAAAACACGCATCGGATCTCTTCTTTACCGCCGGGACTCAGGTCAA
GATCAAGATCGACGGGGTGATCCAGTCGATTGGCGACACCGTCTTCGACACCAACATGTGCGCCGCGGCCATCCATTCCA
TCCTGACAAGAGAGCAGATTGAGTTTTTTGAGACCCACCGCGAGATCGACTTCGGTATCGGGCTGACCGGACAGGGGCGT
TTTCGCGTCAATGCCTTCCACCAGCGCGGCACGCCGGCGATGGTCATCCGCTACCTGACCAGCAAGATCCCAACCATTGA
GGAGCTTGGGCTGCCGGAGATTCTCAAACGCCTGATCCTGCATCGGCGCGGCATCATCCTGATGGTCGGCGCGACCGGCT
CCGGCAAGTCGACCACCCTGGCCGCCATGATCGACCATCGCAACATATCGCGCCCCGGTCACATCCTGACCATCGAGGAC
CCGATCGAATATTTGCACGGGCACAAGAAATCCATCGTCAACCAGCGCGAGCTGGGCATCGACACCATGGATTACGCAAC
CGCGCTGAAAAGCGCGCTGCGCGAAGCCCCTGACGTGATCCTGATCGGCGAGATCCGCACTCGCGAAACCATGGAGGCCG
CCATCGAGTTGTCCGGCACCGGCCACCTGGCAATCTCCACGCTGCACGCGAACAACGCCTACCAGACGATGGAGCGCATC
ATCAACATGTTCCCCAAGGAGATGCATCGGACACTGTTCATGGACCTGTCGCTGAACCTGCGCGCCATCATCTCCCAGCG
CCTGGTGCGCACCGTGGAGGGCAAACGGGTGGCTGCGATCGAAATCCTGATGAACACACCGCACATCAGCGACCTGATCC
GCGAAGGACGCGTGGACGAGATTCGCCAGGCCATGGCCGAGAGCAAGGAAGAAGGCATGACCACCTTCGACGATGCCCTG
CTCAAGCTCTACCGCGACGGGCTCATCTCGCTGGAAGAATGCCTGGCCAATTCCGACTCGGCCGCGAATCTGGAGGCACG
GATCAACTTTGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

52.26

100

0.527
  pilU Pseudomonas stutzeri DSM 10701

50.284

100

0.504
  pilU Vibrio cholerae strain A1552

48.696

98.291

0.479
  pilT Acinetobacter baylyi ADP1

43.44

97.721

0.424
  pilT Pseudomonas aeruginosa PAK

44.012

95.157

0.419
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

95.726

0.413
  pilT Pseudomonas stutzeri DSM 10701

42.151

98.006

0.413
  pilT Neisseria meningitidis 8013

42.56

95.726

0.407
  pilT Neisseria gonorrhoeae MS11

42.308

96.296

0.407
  pilT Acinetobacter baumannii strain A118

43.333

94.017

0.407
  pilT Acinetobacter nosocomialis M2

43.333

94.017

0.407
  pilT Acinetobacter baumannii D1279779

43.333

94.017

0.407
  pilT Vibrio cholerae strain A1552

42.262

95.726

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.262

95.726

0.405
  pilT Legionella pneumophila strain ERS1305867

42.727

94.017

0.402
  pilT Legionella pneumophila strain Lp02

42.727

94.017

0.402