Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Thiowin_RS14880 Genome accession   NZ_CP121472
Coordinates   3303229..3304284 (+) Length   351 a.a.
NCBI ID   WP_328983784.1    Uniprot ID   -
Organism   Thiorhodovibrio winogradskyi strain DSM 6702     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3298229..3309284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiowin_RS14855 (Thiowin_03031) - 3298702..3299022 (-) 321 WP_328983779.1 tyrosine-type recombinase/integrase -
  Thiowin_RS25355 - 3299175..3299435 (+) 261 WP_408034221.1 GNAT family N-acetyltransferase -
  Thiowin_RS14865 (Thiowin_03032) - 3299524..3300195 (+) 672 WP_328983781.1 hypothetical protein -
  Thiowin_RS14870 (Thiowin_03033) - 3300309..3301709 (+) 1401 WP_328983782.1 serine/threonine-protein kinase -
  Thiowin_RS14875 (Thiowin_03034) pilU 3301910..3303181 (+) 1272 WP_328983783.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiowin_RS14880 (Thiowin_03035) pilU 3303229..3304284 (+) 1056 WP_328983784.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiowin_RS14885 (Thiowin_03036) - 3304402..3307848 (+) 3447 WP_328983785.1 ATP-binding protein -
  Thiowin_RS14890 (Thiowin_03037) - 3307956..3308384 (+) 429 WP_328983786.1 hypothetical protein -
  Thiowin_RS14895 (Thiowin_03038) - 3308595..3309158 (+) 564 WP_328983787.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39277.38 Da        Isoelectric Point: 6.6395

>NTDB_id=812327 Thiowin_RS14880 WP_328983784.1 3303229..3304284(+) (pilU) [Thiorhodovibrio winogradskyi strain DSM 6702]
MDITPYLKLMVQKRASDLFFTAGTQVKIKIDGVIQSIGDTIFDNTMCTSAIHSVLTREQIEFFETHREIDFGIGLTGQGR
FRINAFHQRGTAAMVIRYLSSKIPSIEDLGLPDILKRLIMHRRGIILMVGATGSGKSTTLAAMIDHRNNSKPGHILTIED
PIEYLHSHKQSIVNQRELGLDTLDYATALKSALREAPDVIQIGEIRTRETMEAAIELSGTGHLAISTLHANNAYQTMERI
INMFPMEMHKTLFMDLSLNLRAIISQRLVRTLEGKRVAAIEILMNTPHISDLIREGRVDEMRQAMAESKEEGMITFDNAL
LKLYRDGLISLEECLGNADSAANLEARINFG

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=812327 Thiowin_RS14880 WP_328983784.1 3303229..3304284(+) (pilU) [Thiorhodovibrio winogradskyi strain DSM 6702]
ATGGATATCACCCCCTACCTGAAACTCATGGTGCAAAAACGGGCCTCCGACCTGTTTTTCACTGCTGGCACGCAAGTCAA
GATCAAGATTGACGGCGTGATCCAATCGATCGGAGACACTATATTCGATAATACAATGTGCACCAGCGCTATTCATTCGG
TTTTGACCAGGGAGCAAATTGAATTTTTTGAAACCCATCGGGAAATCGACTTCGGCATCGGCCTGACCGGGCAAGGGCGT
TTTCGCATCAACGCTTTTCATCAGCGCGGCACCGCAGCCATGGTGATCCGCTACCTAAGCAGTAAAATTCCAAGTATTGA
GGATCTTGGCCTGCCGGACATTCTCAAGCGTTTAATCATGCACAGACGCGGCATCATTCTGATGGTGGGTGCCACAGGCT
CTGGCAAATCAACCACCCTGGCCGCCATGATCGACCACCGCAACAACTCCAAGCCTGGTCATATTCTAACGATTGAAGAT
CCGATCGAGTATCTGCATTCGCACAAGCAATCCATTGTCAATCAACGCGAACTCGGTCTGGACACCCTAGACTATGCCAC
CGCTCTGAAAAGCGCGCTGCGTGAGGCGCCTGATGTGATTCAAATCGGCGAAATCCGCACCCGCGAGACCATGGAGGCCG
CCATTGAACTGTCCGGCACCGGCCACCTGGCCATTTCCACCCTGCACGCTAATAATGCCTATCAAACCATGGAGCGCATC
ATCAATATGTTCCCCATGGAGATGCACAAAACGCTCTTTATGGATTTGTCCTTGAACCTGCGCGCTATTATTTCTCAACG
CCTGGTGCGCACGTTAGAGGGAAAGCGGGTCGCGGCTATCGAGATTCTGATGAATACTCCGCACATCAGCGACCTGATCC
GCGAAGGACGGGTGGATGAAATGCGCCAGGCCATGGCGGAGAGCAAAGAAGAAGGCATGATCACCTTCGACAATGCTTTG
CTCAAGCTTTACCGTGACGGCCTCATTTCATTGGAAGAGTGCCTTGGCAATGCCGACTCGGCGGCCAACCTGGAGGCGCG
GATCAACTTCGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

51.412

100

0.519
  pilU Pseudomonas stutzeri DSM 10701

50.284

100

0.504
  pilU Vibrio cholerae strain A1552

46.957

98.291

0.462
  pilT Pseudomonas aeruginosa PAK

42.442

98.006

0.416
  pilT Pseudomonas stutzeri DSM 10701

41.86

98.006

0.41
  pilT Acinetobacter baylyi ADP1

41.57

98.006

0.407
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

96.581

0.407
  pilT Legionella pneumophila strain ERS1305867

42.727

94.017

0.402
  pilT Legionella pneumophila strain Lp02

42.727

94.017

0.402
  pilT Neisseria gonorrhoeae MS11

40.462

98.575

0.399
  pilT Neisseria meningitidis 8013

41.617

95.157

0.396
  pilT Acinetobacter baumannii strain A118

42.121

94.017

0.396
  pilT Acinetobacter baumannii D1279779

42.121

94.017

0.396
  pilT Acinetobacter nosocomialis M2

42.121

94.017

0.396
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

94.872

0.382
  pilT Vibrio cholerae strain A1552

40.24

94.872

0.382