Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Thiowin_RS14875 Genome accession   NZ_CP121472
Coordinates   3301910..3303181 (+) Length   423 a.a.
NCBI ID   WP_328983783.1    Uniprot ID   -
Organism   Thiorhodovibrio winogradskyi strain DSM 6702     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3275121..3301675 3301910..3303181 flank 235


Gene organization within MGE regions


Location: 3275121..3303181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiowin_RS14775 (Thiowin_03015) - 3275984..3278953 (+) 2970 WP_328983764.1 DEAD/DEAH box helicase -
  Thiowin_RS14780 (Thiowin_03016) - 3279020..3279277 (+) 258 WP_408034086.1 DUF433 domain-containing protein -
  Thiowin_RS14785 (Thiowin_03017) - 3279280..3279609 (+) 330 WP_328983765.1 DUF5615 family PIN-like protein -
  Thiowin_RS14790 (Thiowin_03018) - 3279648..3284777 (+) 5130 WP_328983766.1 Eco57I restriction-modification methylase domain-containing protein -
  Thiowin_RS14795 (Thiowin_03019) - 3284840..3285256 (+) 417 WP_328983767.1 hypothetical protein -
  Thiowin_RS14800 (Thiowin_03020) - 3285246..3285497 (+) 252 WP_328983768.1 hypothetical protein -
  Thiowin_RS14805 (Thiowin_03021) - 3285599..3285898 (+) 300 WP_328983769.1 BrnA antitoxin family protein -
  Thiowin_RS14810 (Thiowin_03022) - 3285959..3286252 (+) 294 WP_328983770.1 nucleotidyltransferase family protein -
  Thiowin_RS14815 (Thiowin_03023) - 3286242..3286595 (+) 354 WP_328983771.1 DUF86 domain-containing protein -
  Thiowin_RS14820 (Thiowin_03024) - 3286812..3293423 (+) 6612 WP_328983772.1 DEAD/DEAH box helicase -
  Thiowin_RS14825 (Thiowin_03025) - 3293416..3295497 (+) 2082 WP_328983773.1 UvrD-helicase domain-containing protein -
  Thiowin_RS14830 (Thiowin_03026) - 3295650..3295841 (+) 192 WP_328983774.1 hypothetical protein -
  Thiowin_RS14835 (Thiowin_03027) - 3295902..3296480 (-) 579 WP_328983775.1 lytic transglycosylase domain-containing protein -
  Thiowin_RS14840 (Thiowin_03028) - 3296785..3297501 (-) 717 WP_328983776.1 helix-turn-helix domain-containing protein -
  Thiowin_RS14845 (Thiowin_03029) - 3297498..3297950 (-) 453 WP_328983777.1 helix-turn-helix transcriptional regulator -
  Thiowin_RS14850 (Thiowin_03030) - 3298025..3298603 (+) 579 WP_328983778.1 hypothetical protein -
  Thiowin_RS14855 (Thiowin_03031) - 3298702..3299022 (-) 321 WP_328983779.1 tyrosine-type recombinase/integrase -
  Thiowin_RS25355 - 3299175..3299435 (+) 261 WP_408034221.1 GNAT family N-acetyltransferase -
  Thiowin_RS14865 (Thiowin_03032) - 3299524..3300195 (+) 672 WP_328983781.1 hypothetical protein -
  Thiowin_RS14870 (Thiowin_03033) - 3300309..3301709 (+) 1401 WP_328983782.1 serine/threonine-protein kinase -
  Thiowin_RS14875 (Thiowin_03034) pilU 3301910..3303181 (+) 1272 WP_328983783.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 46838.53 Da        Isoelectric Point: 4.8460

>NTDB_id=812326 Thiowin_RS14875 WP_328983783.1 3301910..3303181(+) (pilU) [Thiorhodovibrio winogradskyi strain DSM 6702]
MDRQQAAKFMLDCLRMMMARKGSDLFVSADFPPACKIDGKLTPLSEKPLSAEQTGVLVRSIMNDYQLKEYDANKECNFAI
SPAGIGRFRVNAFVQQGRAGVVLRTIPTEIPEIDDLGLPPVLKDLVLTKRGMILVVGGTGSGKSTSLAAMIGYRNEKTHG
HIITIEDPVEFVHRHRNCLITQREVGVDTDSWDNALVNTLRQAPNVILIGEIRQRETMEHAINFSETGHLCLSTLHANNA
NQALDRIINMFPEERRAQLLMDLSLNLKAVISQRLLPRIGGGRVAAIEIMINSPLIQDLIFKGKVHEIKSVMKRSREQGM
QTFDMALFDLYEAGLITLEDGLRNADSTNELRLKIKLESADATEGDAFGDTQGFALVGEDEEDLPPEQLGKKPSGEDASE
TAFDLDSFDLAEASLDLESDFKR

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=812326 Thiowin_RS14875 WP_328983783.1 3301910..3303181(+) (pilU) [Thiorhodovibrio winogradskyi strain DSM 6702]
ATGGATCGCCAGCAAGCTGCCAAGTTCATGCTCGACTGCCTACGCATGATGATGGCCCGCAAGGGCTCCGACCTCTTTGT
GAGCGCCGATTTCCCCCCTGCCTGCAAGATTGACGGCAAACTCACGCCGCTGAGCGAAAAACCACTGAGCGCGGAACAAA
CCGGCGTGCTGGTACGTTCCATCATGAACGATTACCAGCTCAAGGAATATGACGCCAACAAGGAATGCAACTTCGCCATC
AGCCCTGCCGGCATCGGGCGCTTTCGCGTCAACGCCTTTGTCCAGCAGGGACGTGCCGGCGTGGTCTTGCGCACCATCCC
AACGGAAATACCTGAGATCGACGACCTCGGACTGCCGCCGGTGCTCAAAGACCTGGTGCTGACCAAACGCGGCATGATTT
TGGTCGTCGGCGGCACCGGATCGGGTAAATCCACCAGCTTGGCCGCCATGATCGGCTACCGCAACGAGAAAACCCACGGC
CATATCATCACCATTGAGGATCCGGTGGAATTTGTGCATCGCCACCGCAACTGCCTGATCACTCAGCGTGAGGTGGGGGT
GGATACCGACTCCTGGGACAATGCCTTGGTCAACACCCTACGTCAGGCGCCCAATGTGATTCTGATCGGCGAGATACGTC
AACGCGAGACCATGGAGCATGCCATCAATTTCTCCGAGACCGGCCACCTGTGCCTGTCCACCTTGCATGCAAACAACGCC
AACCAGGCGCTGGACCGCATCATCAACATGTTCCCGGAAGAACGCCGAGCGCAGTTGCTGATGGATCTGTCGCTTAATCT
CAAGGCGGTGATCTCCCAGCGTCTACTGCCGCGCATCGGCGGTGGGCGGGTCGCCGCGATTGAGATCATGATCAACAGCC
CGCTGATTCAGGATCTGATCTTCAAAGGCAAGGTGCATGAGATCAAATCCGTCATGAAGCGCTCGCGCGAGCAGGGCATG
CAGACCTTCGACATGGCGTTGTTCGATCTCTATGAGGCAGGACTCATCACGCTCGAGGACGGCCTGCGCAATGCCGACTC
AACCAACGAACTGCGTTTGAAAATCAAGCTTGAAAGCGCCGACGCGACCGAAGGCGATGCCTTTGGCGACACCCAGGGGT
TCGCCCTGGTGGGCGAGGACGAAGAAGACCTTCCGCCGGAGCAGCTTGGCAAAAAGCCCTCTGGAGAGGATGCCTCGGAA
ACAGCCTTTGACCTCGACAGTTTTGATCTTGCCGAAGCCTCGCTGGACTTGGAATCAGACTTTAAACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.916

87.707

0.534
  pilU Acinetobacter baylyi ADP1

56.034

82.27

0.461
  pilU Vibrio cholerae strain A1552

52.601

81.797

0.43