Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Thiofri_RS23100 Genome accession   NZ_CP121471
Coordinates   5064245..5065300 (-) Length   351 a.a.
NCBI ID   WP_009148880.1    Uniprot ID   H8YZV1
Organism   Thiorhodovibrio frisius strain 970     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5059245..5070300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiofri_RS23080 (Thiofri_04738) murA 5059565..5060848 (+) 1284 WP_009148871.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  Thiofri_RS23085 (Thiofri_04739) hisG 5060853..5061536 (+) 684 WP_051023855.1 ATP phosphoribosyltransferase -
  Thiofri_RS23090 (Thiofri_04740) hisD 5061691..5062995 (+) 1305 WP_009148875.1 histidinol dehydrogenase -
  Thiofri_RS23095 (Thiofri_04741) hisC 5063086..5064189 (+) 1104 WP_009148877.1 histidinol-phosphate transaminase -
  Thiofri_RS23100 (Thiofri_04742) pilU 5064245..5065300 (-) 1056 WP_009148880.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiofri_RS23105 (Thiofri_04743) pilU 5065404..5066675 (-) 1272 WP_009148882.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiofri_RS23110 (Thiofri_04744) - 5066809..5066988 (+) 180 WP_040855827.1 DsrE family protein -
  Thiofri_RS23115 (Thiofri_04745) - 5067048..5068490 (-) 1443 WP_009148883.1 GTPase/DUF3482 domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39221.41 Da        Isoelectric Point: 6.8287

>NTDB_id=812308 Thiofri_RS23100 WP_009148880.1 5064245..5065300(-) (pilU) [Thiorhodovibrio frisius strain 970]
MDITPYLKLMVQKRASDLFFTAGTQVKIKIDGVIQSIGDTVFEPGMCTAAIHSVLTKEQIDFFENHREIDFGIGLSGHGR
FRINAFHQRGTPAMVIRFLTSKIPTIDELGLPEILKRLVMHRRGIILMVGATGSGKSTTLAAMIDHRNSTKPGHILTIED
PIEYIHAHKQSIINQRELGIDTKDYATALKSSLREAPDVILIGEIRTRETMEAAIELSGTGHLAISTLHANNAYQTMERI
INMFPMEMHKTLFMDLSLNLRAIISQRLVRTVEGKRVAAIEILMNTPHISDLIREGRVDEMRQAMAESKEEGMVTFDEAL
LKLYRDGRISLDECLGNADSAANLEARINFG

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=812308 Thiofri_RS23100 WP_009148880.1 5064245..5065300(-) (pilU) [Thiorhodovibrio frisius strain 970]
ATGGATATCACCCCTTACCTGAAATTGATGGTGCAAAAGCGCGCATCGGATTTGTTCTTTACTGCCGGCACCCAAGTCAA
GATCAAAATCGACGGCGTCATCCAGTCAATCGGCGATACCGTTTTTGAGCCTGGAATGTGCACTGCGGCCATTCACTCAG
TGCTGACAAAAGAGCAGATTGATTTCTTCGAGAATCACCGTGAAATCGATTTTGGCATTGGCTTGTCTGGGCATGGGCGC
TTCCGCATTAACGCCTTTCACCAGCGGGGCACCCCCGCCATGGTTATTCGCTTCCTGACCAGCAAGATACCCACCATTGA
TGAGCTTGGACTACCGGAGATTCTCAAGCGACTAGTGATGCATAGACGCGGCATCATCCTGATGGTGGGCGCTACCGGAT
CAGGTAAATCGACCACCCTTGCGGCCATGATTGATCACAGGAATAGCACGAAACCCGGGCATATTCTGACCATTGAGGAT
CCCATTGAGTATATTCACGCGCACAAGCAATCCATCATTAACCAGCGCGAATTGGGTATCGACACCAAGGACTATGCCAC
TGCGCTAAAAAGCTCCTTGCGCGAAGCACCGGATGTGATTCTGATCGGCGAGATCCGCACCCGCGAGACCATGGAAGCGG
CCATTGAACTCTCAGGTACTGGCCATCTGGCCATCTCTACCCTGCATGCGAACAATGCCTATCAGACCATGGAACGCATC
ATTAATATGTTCCCGATGGAAATGCACAAAACGCTGTTCATGGATTTGTCTCTAAACTTGCGTGCCATCATCTCCCAGCG
CCTGGTGCGCACTGTGGAGGGCAAGCGGGTCGCGGCCATCGAGATTTTGATGAATACCCCGCACATCAGCGATTTGATCC
GCGAGGGGCGCGTGGATGAAATGCGTCAGGCGATGGCAGAAAGCAAAGAAGAAGGCATGGTGACCTTCGACGAGGCGTTG
CTCAAGCTCTACCGCGATGGACGTATTTCGCTGGATGAATGCCTCGGCAATGCCGATTCGGCGGCGAATCTGGAGGCGCG
GATTAACTTTGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H8YZV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

51.695

100

0.521
  pilU Pseudomonas stutzeri DSM 10701

50.708

100

0.51
  pilU Vibrio cholerae strain A1552

48.116

98.291

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.86

97.436

0.427
  pilT Pseudomonas aeruginosa PAK

42.151

98.006

0.413
  pilT Pseudomonas stutzeri DSM 10701

41.279

98.006

0.405
  pilT Acinetobacter baylyi ADP1

41.471

96.866

0.402
  pilT Acinetobacter baumannii D1279779

42.121

94.017

0.396
  pilT Acinetobacter baumannii strain A118

42.121

94.017

0.396
  pilT Acinetobacter nosocomialis M2

42.121

94.017

0.396
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

94.872

0.387
  pilT Vibrio cholerae strain A1552

40.841

94.872

0.387
  pilT Legionella pneumophila strain Lp02

41.212

94.017

0.387
  pilT Legionella pneumophila strain ERS1305867

41.212

94.017

0.387
  pilT Neisseria gonorrhoeae MS11

40.785

94.302

0.385
  pilT Neisseria meningitidis 8013

40.785

94.302

0.385