Detailed information NTG0812309

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Thiofri_RS23105
• Genome accession: NZ_CP121471
• Coordinates: 5065404..5066675 (-)
• Length: 423 a.a.
• NCBI ID: WP_009148882.1
• Uniprot ID: H8YZV2
• Organism: Thiorhodovibrio frisius strain 970
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5060404..5071675

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Thiofri_RS23085 (Thiofri_04739)	hisG	5060853..5061536 (+)	684	WP_051023855.1	ATP phosphoribosyltransferase	-
Thiofri_RS23090 (Thiofri_04740)	hisD	5061691..5062995 (+)	1305	WP_009148875.1	histidinol dehydrogenase	-
Thiofri_RS23095 (Thiofri_04741)	hisC	5063086..5064189 (+)	1104	WP_009148877.1	histidinol-phosphate transaminase	-
Thiofri_RS23100 (Thiofri_04742)	pilU	5064245..5065300 (-)	1056	WP_009148880.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Thiofri_RS23105 (Thiofri_04743)	pilU	5065404..5066675 (-)	1272	WP_009148882.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Thiofri_RS23110 (Thiofri_04744)	-	5066809..5066988 (+)	180	WP_040855827.1	DsrE family protein	-
Thiofri_RS23115 (Thiofri_04745)	-	5067048..5068490 (-)	1443	WP_009148883.1	GTPase/DUF3482 domain-containing protein	-
Thiofri_RS23120 (Thiofri_04746)	-	5068490..5070649 (-)	2160	WP_009148886.1	DUF2868 domain-containing protein	-

Sequence

Protein

• Length: 423 a.a.
• Molecular weight: 46728.46 Da
• Isoelectric Point: 4.7366

>NTDB_id=812309 Thiofri_RS23105 WP_009148882.1 5065404..5066675(-) (pilU) [Thiorhodovibrio frisius strain 970]
MDRQQAAKFMLDCLRMMMARKGSDLFVSADFPPACKIDGKLTPLSEKPLSAEQTGVLVRSIMNDYQLKEYDANKECNFAI
SPPGIGRFRVNAFVQQGRSGVVLRTIPTEIPEIDALGLPPVLKDLVLTKRGMILLVGGTGSGKSTSLAAMLGYRNEKTHG
HIITIEDPVEFVHQHRNCLITQREVGVDTDSWDNALVNTLRQAPNVILIGEIRQRETMEHAINFSETGHLCLSTLHANNA
NQALDRIINLFPEERRSQLLMDLSLNLKAVISQRLLPRIGGGRVAAIEVMINSPLIQDLIFKGKVHEIKAIMKRSRELGM
QTFDMALFDLYEAGLITLEDGLRNADSMNELRLKIKLESADATEGDAFGDTDGLRLVGEDEEDLPPGELGNKPAADDASE
SAFDLDSFDLAEASLDLESEFKQ

Nucleotide

• Length: 1272 bp

>NTDB_id=812309 Thiofri_RS23105 WP_009148882.1 5065404..5066675(-) (pilU) [Thiorhodovibrio frisius strain 970]
ATGGATCGTCAGCAAGCCGCCAAGTTCATGCTCGACTGTCTGCGCATGATGATGGCCCGCAAGGGCTCCGACCTCTTCGT
CAGCGCTGACTTTCCGCCCGCCTGCAAAATCGATGGCAAACTCACGCCGCTGAGTGAAAAACCCCTGAGCGCTGAGCAGA
CTGGCGTGTTGGTGCGTTCCATCATGAACGACTACCAGCTTAAGGAATACGACGCCAACAAGGAATGCAACTTCGCCATC
AGCCCGCCTGGCATCGGGCGTTTCCGCGTCAATGCTTTCGTGCAACAGGGGCGCTCCGGCGTTGTGCTGCGCACTATCCC
GACCGAAATTCCCGAGATCGACGCACTTGGCCTGCCACCGGTGCTCAAGGATCTGGTGCTCACCAAGCGCGGCATGATTC
TGCTGGTAGGCGGCACCGGCTCGGGCAAATCCACCAGCCTGGCCGCGATGCTCGGCTACCGCAACGAGAAGACCCATGGC
CATATCATCACCATCGAGGACCCGGTGGAGTTCGTGCATCAGCACCGAAACTGCCTGATCACCCAGCGTGAGGTGGGCGT
GGATACCGACTCCTGGGACAACGCTCTGGTTAATACTCTGCGTCAGGCGCCCAATGTGATTCTGATCGGCGAGATTCGCC
AGCGCGAGACCATGGAGCATGCAATCAACTTCTCCGAGACCGGCCATTTGTGCCTGTCTACACTGCATGCCAACAACGCC
AACCAGGCACTGGACCGCATCATCAATTTGTTTCCTGAGGAACGCCGCTCACAGTTGCTGATGGATCTGTCGCTCAATCT
AAAAGCCGTCATTTCCCAGCGTCTGCTGCCGCGCATTGGCGGAGGAAGGGTGGCGGCGATTGAGGTCATGATCAACAGCC
CGCTGATTCAGGATCTGATCTTCAAAGGCAAGGTGCATGAAATCAAAGCCATCATGAAGCGCTCGCGCGAGCTTGGCATG
CAGACCTTCGACATGGCCTTGTTTGATCTCTACGAGGCCGGACTGATCACGCTCGAAGACGGTCTGCGCAATGCTGATTC
CATGAACGAGCTGCGCCTGAAAATCAAGCTTGAAAGTGCCGATGCCACCGAGGGCGATGCCTTTGGCGACACTGATGGTC
TCAGGCTGGTCGGCGAGGACGAGGAAGACCTGCCCCCCGGCGAACTGGGCAATAAACCGGCGGCGGACGATGCATCTGAG
AGCGCGTTCGACCTTGACAGCTTCGACCTGGCCGAGGCCTCGCTCGATTTGGAATCGGAGTTCAAGCAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (121-276)

3D structure

• AlphaFold DB: H8YZV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.401	82.033	0.52
pilU	Acinetobacter baylyi ADP1	56.034	82.27	0.461
pilU	Vibrio cholerae strain A1552	53.179	81.797	0.435