Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P3S51_RS03105 Genome accession   NZ_CP121303
Coordinates   650157..651194 (-) Length   345 a.a.
NCBI ID   WP_154772396.1    Uniprot ID   -
Organism   Acinetobacter sp. ANC 7201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645157..656194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3S51_RS03075 (P3S51_03075) - 645929..646384 (+) 456 WP_154772390.1 bacteriohemerythrin -
  P3S51_RS03080 (P3S51_03080) - 646540..647622 (+) 1083 WP_248103105.1 hypothetical protein -
  P3S51_RS03085 (P3S51_03085) - 647622..647948 (+) 327 WP_154772392.1 RnfH family protein -
  P3S51_RS03090 (P3S51_03090) - 647990..648388 (-) 399 WP_248103108.1 outer membrane protein assembly factor BamE -
  P3S51_RS03095 (P3S51_03095) fur 648500..648937 (+) 438 WP_154772394.1 ferric iron uptake transcriptional regulator -
  P3S51_RS03100 (P3S51_03100) pilU 649002..650123 (-) 1122 WP_154772395.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P3S51_RS03105 (P3S51_03105) pilT 650157..651194 (-) 1038 WP_154772396.1 type IV pilus twitching motility protein PilT Machinery gene
  P3S51_RS03110 (P3S51_03110) - 651318..652004 (+) 687 WP_154772397.1 YggS family pyridoxal phosphate-dependent enzyme -
  P3S51_RS03115 (P3S51_03115) - 652115..652537 (+) 423 WP_311918273.1 hypothetical protein -
  P3S51_RS03120 (P3S51_03120) - 652569..656165 (-) 3597 WP_311918274.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38364.01 Da        Isoelectric Point: 6.6655

>NTDB_id=811742 P3S51_RS03105 WP_154772396.1 650157..651194(-) (pilT) [Acinetobacter sp. ANC 7201]
MDITELLAFSAKNNASDLHLSAGMPPMIRVDGEVRKINLPALEHKEVHKLVYDIMNDKQRRDYEEKLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMTQIFKDICEYPRGIVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVNKGVISAQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=811742 P3S51_RS03105 WP_154772396.1 650157..651194(-) (pilT) [Acinetobacter sp. ANC 7201]
ATGGACATTACTGAACTACTCGCATTTTCTGCAAAAAATAATGCATCAGATTTACATCTATCTGCGGGTATGCCACCAAT
GATTCGTGTGGATGGTGAAGTACGTAAAATTAATTTACCAGCCTTAGAACACAAAGAAGTTCATAAACTTGTTTATGACA
TTATGAACGATAAACAACGTCGCGATTATGAAGAAAAATTAGAAACTGACTTTTCATTTGAAGTTCCTGGCGTAGCTCGT
TTCCGTGTGAACGCATTCAACCAAAACCGTGGTGCAGGTGCTGTATTCCGTACAATTCCATCAAAAGTATTAACGATGGA
AGATTTAGGCATGACTCAAATCTTTAAAGATATTTGTGAATATCCACGTGGGATTGTTCTGGTTACAGGGCCAACTGGTT
CGGGTAAATCAACAACACTTGCAGCAATGATTGATTACATTAACGACAACCGTTATGACCATATTTTAACTGTCGAAGAC
CCAATCGAGTTTGTGCACCAATCTAAAAAATGCTTGGTGAACCAACGTGAAGTACACCGTGACACACATGGCTTTAATGA
AGCACTTCGTTCAGCGCTGCGTGAAGATCCAGATATTATCTTAGTTGGTGAGATGCGTGACCTTGAAACGATTCGCTTAG
CGCTAACTGCTGCGGAAACAGGTCACTTAGTATTTGGTACGCTACATACCACATCTGCGGCTAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGTGCCATGTTGTCAGAATCATTACAAGCCGTTATTTCTCAAAC
ACTCTTAAAGAAAAATGGTGGCGGTCGTGTTGCTGCACATGAAATTATGATTGGTATTCCTGCTATTCGTAACTTAATTC
GTGAAAACAAAGTAGCGCAAATGTATTCTGCAATTCAAACAGGTGCAAACTACGGCATGACTACGCTTGACCAAAGCTTA
AAAACTTTAGTCAACAAAGGTGTAATTAGCGCACAAACTGCACGTACTGCAGCAAAACAGCCTGAAACATTCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

94.783

100

0.948
  pilT Acinetobacter baumannii strain A118

94.783

100

0.948
  pilT Acinetobacter nosocomialis M2

94.783

100

0.948
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

76.163

99.71

0.759
  pilT Legionella pneumophila strain ERS1305867

76.163

99.71

0.759
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.238

97.391

0.684
  pilT Vibrio cholerae strain A1552

70.238

97.391

0.684
  pilT Neisseria meningitidis 8013

67.536

100

0.675
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

39.067

99.42

0.388