Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P8934_RS00945 Genome accession   NZ_CP121193
Coordinates   193571..194605 (+) Length   344 a.a.
NCBI ID   WP_106232253.1    Uniprot ID   -
Organism   Halomonas sp. CKK8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 188571..199605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8934_RS00915 (P8934_00915) - 188966..189418 (-) 453 WP_278032496.1 DUF4426 domain-containing protein -
  P8934_RS00920 (P8934_00920) metW 189464..190063 (-) 600 WP_278033979.1 methionine biosynthesis protein MetW -
  P8934_RS00925 (P8934_00925) - 190063..191220 (-) 1158 WP_278032497.1 homoserine O-acetyltransferase -
  P8934_RS00930 (P8934_00930) - 191258..191851 (-) 594 WP_133637676.1 YggT family protein -
  P8934_RS00935 (P8934_00935) proC 191913..192737 (-) 825 WP_278032498.1 pyrroline-5-carboxylate reductase -
  P8934_RS00940 (P8934_00940) - 192800..193507 (-) 708 WP_278032499.1 YggS family pyridoxal phosphate-dependent enzyme -
  P8934_RS00945 (P8934_00945) pilT 193571..194605 (+) 1035 WP_106232253.1 type IV pilus twitching motility protein PilT Machinery gene
  P8934_RS00950 (P8934_00950) pilU 194637..195779 (+) 1143 WP_278032500.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P8934_RS00955 (P8934_00955) - 195793..196923 (-) 1131 WP_278032501.1 anhydro-N-acetylmuramic acid kinase -
  P8934_RS00960 (P8934_00960) tyrS 197037..198236 (+) 1200 WP_278032502.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37960.54 Da        Isoelectric Point: 6.3154

>NTDB_id=810890 P8934_RS00945 WP_106232253.1 193571..194605(+) (pilT) [Halomonas sp. CKK8]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDIMADRQRRDYEEFFETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPTEVLTLEDLGMGPVFRQLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDNRFEHILTIED
PVEFVHRSKRCLVNQREVHRDTHGFAPALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVISQALLKRQGGGRVAAHEILVATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLDASL
ARLVADNMVAREEAQAKAKGVLAS

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=810890 P8934_RS00945 WP_106232253.1 193571..194605(+) (pilT) [Halomonas sp. CKK8]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAACGCCTCGGACCTCCATCTCTCGGCGGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACATCCGCCGGCTCAACGTGCCGGCCATGGAGGACCGCGAGGTCCGCAAGCTGATCTACGACA
TCATGGCGGACCGCCAGCGCCGCGACTACGAGGAGTTCTTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTCCGTGTCAACGTCTTCAACCAGGCCCGCGGAGCCGGGGCGGTGTTCCGGACCATCCCCACCGAGGTGCTGACCCTGGA
GGACCTGGGCATGGGGCCGGTCTTCCGCCAGCTCTCCATGCTGCCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACCACCCTGGCGGCGATGATCGACTACATCAACGACAACCGCTTCGAGCACATCCTCACCATCGAGGAC
CCAGTAGAGTTCGTGCACCGCAGCAAGCGCTGCCTGGTCAACCAGCGTGAGGTGCACCGCGATACCCATGGCTTCGCCCC
GGCGCTGCGCAGCGCCCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGCTGCGCGACCTGGAGACGATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCATACCACTTCCGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTTCCCGGCGAGGAGAAGTCCATGGTGCGCTCGATGCTCTCCGAGTCACTGCAGGCGGTGATCTCCCAGGC
CCTGCTCAAGCGCCAGGGCGGCGGCCGGGTGGCGGCCCACGAGATCCTGGTGGCCACCGCGGCGGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGATCCAGACCGGCGGCAACCTGGGCATGCAGACCCTGGATGCCTCGCTG
GCGCGCCTGGTGGCGGACAACATGGTGGCGCGCGAGGAGGCCCAGGCGAAGGCCAAGGGCGTGCTGGCGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.351

98.547

0.782
  pilT Pseudomonas aeruginosa PAK

79.056

98.547

0.779
  pilT Acinetobacter baumannii D1279779

76.401

98.547

0.753
  pilT Acinetobacter baumannii strain A118

76.401

98.547

0.753
  pilT Acinetobacter nosocomialis M2

76.106

98.547

0.75
  pilT Acinetobacter baylyi ADP1

76.106

98.547

0.75
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

65.396

99.128

0.648
  pilT Neisseria gonorrhoeae MS11

65.103

99.128

0.645
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.212

98.547

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

38.824

98.837

0.384