Detailed information NTG0810891

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P8934_RS00950
• Genome accession: NZ_CP121193
• Coordinates: 194637..195779 (+)
• Length: 380 a.a.
• NCBI ID: WP_278032500.1
• Uniprot ID: -
• Organism: Halomonas sp. CKK8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 189637..200779

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8934_RS00925 (P8934_00925)	-	190063..191220 (-)	1158	WP_278032497.1	homoserine O-acetyltransferase	-
P8934_RS00930 (P8934_00930)	-	191258..191851 (-)	594	WP_133637676.1	YggT family protein	-
P8934_RS00935 (P8934_00935)	proC	191913..192737 (-)	825	WP_278032498.1	pyrroline-5-carboxylate reductase	-
P8934_RS00940 (P8934_00940)	-	192800..193507 (-)	708	WP_278032499.1	YggS family pyridoxal phosphate-dependent enzyme	-
P8934_RS00945 (P8934_00945)	pilT	193571..194605 (+)	1035	WP_106232253.1	type IV pilus twitching motility protein PilT	Machinery gene
P8934_RS00950 (P8934_00950)	pilU	194637..195779 (+)	1143	WP_278032500.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P8934_RS00955 (P8934_00955)	-	195793..196923 (-)	1131	WP_278032501.1	anhydro-N-acetylmuramic acid kinase	-
P8934_RS00960 (P8934_00960)	tyrS	197037..198236 (+)	1200	WP_278032502.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 41920.08 Da
• Isoelectric Point: 5.6138

>NTDB_id=810891 P8934_RS00950 WP_278032500.1 194637..195779(+) (pilU) [Halomonas sp. CKK8]
MTAKEWLYQLLDIMVQKEASDLLISVNAPPTLKMAGQLTPLGDQGLSVAQVTELVGASIPDAVLERFRVEHEANFALSLK
DKGRFRVSAFQQRNQMAMVIRRIGFEIPHLEELSLPSTLGELANNKRGLVFVVGGTGTGKSTTLASMIQQRNETLGGHII
SVEDPIEYVHPHRKAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLHANNANQA
LDRIINFFPIERHSQVWLDLSLNLRAIVAQQLLPTVDGGRCPAIEIMLKSPLIGDLVRKGEVSEIKNVMARSRDLGMQTF
DQALFDLFKAGKITEEVALVHADSANDLRMMIKYGDESSEGVAGAREAATHLSLRGEDDF

Nucleotide

• Length: 1143 bp

>NTDB_id=810891 P8934_RS00950 WP_278032500.1 194637..195779(+) (pilU) [Halomonas sp. CKK8]
ATGACGGCAAAAGAATGGCTCTACCAGCTGCTGGACATCATGGTGCAGAAGGAGGCGTCGGATCTGCTGATCTCGGTGAA
CGCGCCGCCGACCCTGAAGATGGCCGGCCAGCTCACCCCCCTGGGGGACCAGGGGCTCAGCGTGGCGCAGGTCACCGAAC
TGGTCGGTGCCTCGATCCCCGATGCGGTGCTGGAACGCTTCCGTGTCGAGCACGAGGCCAACTTCGCACTGAGCCTCAAG
GACAAGGGGCGCTTCCGCGTCAGTGCCTTCCAGCAGCGCAACCAGATGGCCATGGTGATCCGGCGCATCGGCTTCGAGAT
TCCCCACCTGGAGGAGCTGTCGCTGCCTTCCACGCTCGGCGAGCTGGCCAACAACAAGCGTGGCCTGGTGTTCGTGGTCG
GGGGGACCGGGACCGGCAAGTCGACCACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACCCTCGGCGGCCACATCATC
AGCGTCGAGGATCCCATCGAGTACGTGCACCCGCACCGCAAGGCGATCGTCAACCAGCGCGAGGTCGGTATCGACACCGA
ATCCTTCGAGGTGGCGCTGAAGAATACCCTGCGCCAGGCACCGGACGTGATCCTGATCGGCGAGGTGCGCACCCGGGAGA
CCATGGAACACGCCCTGACCTTCGCCGAGACCGGCCACCTCTGCCTGGCGACCTTGCACGCCAACAACGCCAACCAGGCG
CTGGACCGGATCATCAACTTCTTTCCTATCGAGCGACACTCCCAGGTGTGGCTCGACCTCTCGCTCAACCTGCGGGCCAT
CGTCGCCCAGCAATTGCTGCCCACGGTGGATGGCGGGCGCTGCCCGGCCATCGAGATCATGCTCAAGTCGCCGCTGATCG
GCGACCTGGTGCGCAAGGGGGAGGTCTCCGAGATCAAGAACGTCATGGCCCGCTCGCGGGATCTCGGCATGCAGACCTTC
GACCAGGCGCTCTTCGACCTCTTCAAGGCCGGCAAGATCACCGAGGAGGTGGCGCTGGTGCACGCCGACTCGGCCAATGA
CCTGCGCATGATGATCAAGTACGGCGACGAGAGCAGCGAGGGCGTGGCCGGGGCCAGGGAGGCGGCCACCCACCTGTCGC
TGCGCGGCGAGGACGACTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (49-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.678	92.368	0.579
pilU	Vibrio cholerae strain A1552	58.38	94.211	0.55
pilU	Acinetobacter baylyi ADP1	54.958	92.895	0.511
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.077	96.316	0.405
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.896	86.053	0.361
pilT	Vibrio cholerae strain A1552	41.896	86.053	0.361
pilT	Legionella pneumophila strain Lp02	41.515	86.842	0.361
pilT	Legionella pneumophila strain ERS1305867	41.515	86.842	0.361