Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P8S54_RS08620 Genome accession   NZ_CP121119
Coordinates   1890526..1891569 (-) Length   347 a.a.
NCBI ID   WP_281441394.1    Uniprot ID   A0AAX3S1J8
Organism   Halomonas sp. M1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1885526..1896569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8S54_RS08605 (P8S55_08575) tyrS 1886877..1888076 (-) 1200 WP_281441391.1 tyrosine--tRNA ligase -
  P8S54_RS08610 (P8S55_08580) - 1888195..1889334 (+) 1140 WP_281441392.1 anhydro-N-acetylmuramic acid kinase -
  P8S54_RS08615 (P8S55_08585) pilU 1889359..1890522 (-) 1164 WP_281441393.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P8S54_RS08620 (P8S55_08590) pilT 1890526..1891569 (-) 1044 WP_281441394.1 type IV pilus twitching motility protein PilT Machinery gene
  P8S54_RS08625 (P8S55_08595) - 1891604..1892299 (+) 696 WP_281441395.1 YggS family pyridoxal phosphate-dependent enzyme -
  P8S54_RS08630 (P8S55_08600) proC 1892387..1893211 (+) 825 WP_281441396.1 pyrroline-5-carboxylate reductase -
  P8S54_RS08635 (P8S55_08605) - 1893241..1893834 (+) 594 WP_009101452.1 YggT family protein -
  P8S54_RS08640 (P8S55_08610) - 1893901..1895109 (+) 1209 WP_281441397.1 homoserine O-acetyltransferase -
  P8S54_RS08645 (P8S55_08615) metW 1895109..1895702 (+) 594 WP_281441398.1 methionine biosynthesis protein MetW -
  P8S54_RS08650 (P8S55_08620) - 1895738..1896187 (+) 450 WP_009101446.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38231.66 Da        Isoelectric Point: 6.0557

>NTDB_id=810212 P8S54_RS08620 WP_281441394.1 1890526..1891569(-) (pilT) [Halomonas sp. M1]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDNSEVRRLIYDIMNDQQRRDYEEHLETDFSFDVPGVAR
FRVNAFNQARGAGAVFRTIPNEVLSMQALGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHASKRCLINQREVHRDTRSFNSALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVVSQTLLKRQGGGRVAAHEILIATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLNASL
ARLVKEGMVSMEDAQTRAKGTLSLGDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=810212 P8S54_RS08620 WP_281441394.1 1890526..1891569(-) (pilT) [Halomonas sp. M1]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCGGACCTGCACCTTTCTGCGGGTTTGCCGCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTACCGGCGATGGATAACAGCGAAGTCCGGCGGTTGATCTACGACA
TCATGAACGATCAGCAGCGCCGCGACTACGAAGAACACTTGGAAACCGACTTCTCTTTTGACGTGCCAGGAGTGGCGCGT
TTTCGGGTCAATGCGTTTAACCAGGCGCGAGGTGCTGGGGCGGTTTTTCGTACTATCCCTAATGAAGTGCTTTCGATGCA
GGCCCTGGGCCTAGGCGAGGTGTTCGAGCGCTTAGCCATGCTGCCCCGTGGCTTGGTACTGGTTACCGGGCCAACAGGGT
CGGGTAAAAGCACCACGCTTGCCGCGATGATTGACTACATCAACGACCACCGCTTTGAGCATATTTTAACGATTGAAGAC
CCGGTGGAGTTTGTTCATGCCAGTAAGCGTTGCCTGATCAATCAGCGCGAAGTGCACCGGGACACGCGTAGTTTCAACAG
CGCATTACGCAGCGCCCTGCGTGAAGACCCGGACGTTATTTTAGTAGGTGAGCTGCGCGACCTAGAAACAATTCGCTTAG
CGCTAACCGCTGCTGAAACAGGCCATCTAGTGTTTGGAACCCTGCATACTACTTCTGCAGCAAAGACCATCGACCGAATT
ATTGATGTCTTCCCAGGGGAAGAGAAATCCATGGTGCGCTCAATGCTGTCGGAGTCGCTACAGGCCGTTGTGTCGCAAAC
GTTGCTAAAACGCCAAGGCGGCGGGCGCGTAGCGGCTCACGAAATATTGATTGCCACCGCCGCTGTTCGCAATTTAATCC
GTGAAGATAAAGTGGCACAGATTTATTCGGCCATTCAAACCGGCGGAAACTTGGGCATGCAAACCTTAAACGCGTCGCTT
GCAAGGCTAGTAAAAGAGGGCATGGTGAGTATGGAAGACGCTCAGACGCGGGCAAAAGGCACGCTTTCACTTGGGGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.171

97.695

0.764
  pilT Pseudomonas aeruginosa PAK

77.876

97.695

0.761
  pilT Acinetobacter nosocomialis M2

76.991

97.695

0.752
  pilT Acinetobacter baumannii D1279779

76.991

97.695

0.752
  pilT Acinetobacter baumannii strain A118

76.991

97.695

0.752
  pilT Acinetobacter baylyi ADP1

75.291

99.135

0.746
  pilT Legionella pneumophila strain Lp02

71.598

97.406

0.697
  pilT Legionella pneumophila strain ERS1305867

71.598

97.406

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.118

0.674
  pilT Vibrio cholerae strain A1552

69.436

97.118

0.674
  pilT Neisseria meningitidis 8013

66.377

99.424

0.66
  pilT Neisseria gonorrhoeae MS11

66.087

99.424

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.687

97.695

0.524
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

40.882

97.983

0.401
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392