Detailed information NTG0810211

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P8S54_RS08615
• Genome accession: NZ_CP121119
• Coordinates: 1889359..1890522 (-)
• Length: 387 a.a.
• NCBI ID: WP_281441393.1
• Uniprot ID: A0AAX3S335
• Organism: Halomonas sp. M1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1884359..1895522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8S54_RS08605 (P8S55_08575)	tyrS	1886877..1888076 (-)	1200	WP_281441391.1	tyrosine--tRNA ligase	-
P8S54_RS08610 (P8S55_08580)	-	1888195..1889334 (+)	1140	WP_281441392.1	anhydro-N-acetylmuramic acid kinase	-
P8S54_RS08615 (P8S55_08585)	pilU	1889359..1890522 (-)	1164	WP_281441393.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P8S54_RS08620 (P8S55_08590)	pilT	1890526..1891569 (-)	1044	WP_281441394.1	type IV pilus twitching motility protein PilT	Machinery gene
P8S54_RS08625 (P8S55_08595)	-	1891604..1892299 (+)	696	WP_281441395.1	YggS family pyridoxal phosphate-dependent enzyme	-
P8S54_RS08630 (P8S55_08600)	proC	1892387..1893211 (+)	825	WP_281441396.1	pyrroline-5-carboxylate reductase	-
P8S54_RS08635 (P8S55_08605)	-	1893241..1893834 (+)	594	WP_009101452.1	YggT family protein	-
P8S54_RS08640 (P8S55_08610)	-	1893901..1895109 (+)	1209	WP_281441397.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42248.33 Da
• Isoelectric Point: 6.7413

>NTDB_id=810211 P8S54_RS08615 WP_281441393.1 1889359..1890522(-) (pilU) [Halomonas sp. M1]
MSEPKSGITASQWLHQLLAIMVEQQASDLLISVGAPASLKTPQGLVPLGRQPLSSSQVRSLVVNAIPEMQRERFALEHEA
NFALSLEQKGRFRVSAFQQRNEPAMVVRRIAQEIPTLSALGVPSQLAELANAKRGLVLVVGGTGTGKSTTLAAMIQERNV
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQIRMDLSLNLRAVVAQQLLPTQQGGRCAAIEIMLSSPRVVDLIRKGRIDELKSAMAASR
DAGMQTFDQALYALYQAEQISQQVALAHADSANDLRLMIKHGDAAASGTPLAAQISPHLALRDGDDY

Nucleotide

• Length: 1164 bp

>NTDB_id=810211 P8S54_RS08615 WP_281441393.1 1889359..1890522(-) (pilU) [Halomonas sp. M1]
ATGAGCGAGCCGAAAAGCGGTATAACGGCCTCCCAGTGGTTGCATCAGTTGCTGGCTATTATGGTTGAGCAGCAGGCATC
AGACCTGCTGATTTCGGTCGGCGCGCCGGCAAGCTTAAAAACGCCTCAGGGGTTAGTGCCTCTGGGGCGGCAGCCGCTTT
CCTCAAGCCAAGTGCGCAGCCTGGTGGTCAATGCGATACCCGAAATGCAGCGTGAGCGGTTTGCGTTAGAGCACGAGGCG
AATTTCGCCCTAAGCCTTGAGCAAAAAGGCCGCTTTCGGGTCAGCGCTTTTCAGCAGCGTAATGAGCCTGCCATGGTGGT
GCGCCGCATTGCTCAAGAAATTCCCACACTCTCGGCACTGGGTGTGCCCAGTCAGCTCGCTGAACTCGCTAACGCCAAGC
GCGGGCTGGTGCTGGTGGTGGGAGGAACGGGCACGGGTAAATCGACCACGCTAGCAGCCATGATTCAAGAGCGTAACGTC
TCGGTGGGTGGGCATATCATCACCATCGAAGACCCTATTGAGTATTTGCATCCGCACCAGCGCGGTATCGTCAATCAGCG
CGAGGTGGGGGTAGATACAGAGTCGTTTGAAGTGGCGCTCAAGAATACGCTTCGCCAGGCGCCCGACGTGATTCTGATTG
GTGAGGTGCGCACCCGGGAAACGATGGAGCACGCGCTGACCTTTGCTGAAACAGGGCATCTTTGCCTTGCCACGCTACAC
GCTAACAATGCTAATCAAGCGTTGGAGCGCATTCTCCACTTCTTTCCTCACGAAAGGCATGAGCAAATACGGATGGACTT
ATCGCTCAACCTTCGTGCGGTGGTGGCGCAGCAGTTGTTGCCTACTCAGCAGGGCGGTCGCTGTGCGGCCATTGAGATTA
TGCTTTCGTCACCGCGCGTGGTGGATCTGATACGCAAAGGCCGCATCGACGAGTTGAAGTCGGCCATGGCAGCGTCCCGA
GACGCAGGCATGCAAACCTTCGACCAAGCGCTCTACGCGCTCTATCAAGCGGAGCAAATCAGCCAGCAGGTAGCGTTGGC
CCATGCCGACTCGGCAAACGATTTACGACTGATGATCAAGCATGGCGACGCGGCTGCCAGCGGCACTCCGTTGGCAGCGC
AGATATCTCCCCATTTGGCGCTGCGTGATGGGGATGATTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (22-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.626	96.641	0.576
pilU	Vibrio cholerae strain A1552	58.217	92.765	0.54
pilU	Acinetobacter baylyi ADP1	55.65	91.473	0.509
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.779	95.866	0.401
pilT	Legionella pneumophila strain Lp02	43.03	85.271	0.367
pilT	Legionella pneumophila strain ERS1305867	43.03	85.271	0.367