Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P5S46_RS17885 Genome accession   NZ_CP120942
Coordinates   3860823..3861857 (+) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas caviae strain AC1520     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3836666..3897659 3860823..3861857 within 0


Gene organization within MGE regions


Location: 3836666..3897659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5S46_RS17750 (P5S46_17750) artM 3837751..3838413 (-) 663 WP_010673075.1 arginine ABC transporter permease ArtM -
  P5S46_RS17755 (P5S46_17755) artQ 3838416..3839066 (-) 651 WP_042014905.1 arginine ABC transporter permease ArtQ -
  P5S46_RS17760 (P5S46_17760) - 3839070..3839807 (-) 738 WP_010673073.1 arginine ABC transporter substrate-binding protein -
  P5S46_RS17765 (P5S46_17765) - 3839832..3840572 (-) 741 WP_041214472.1 ATP-binding cassette domain-containing protein -
  P5S46_RS17770 (P5S46_17770) argR 3840773..3841243 (+) 471 WP_005308670.1 transcriptional regulator ArgR -
  P5S46_RS17775 (P5S46_17775) - 3841323..3842228 (-) 906 WP_042864452.1 Dyp-type peroxidase -
  P5S46_RS17780 (P5S46_17780) - 3842318..3843352 (-) 1035 WP_277856308.1 ABC transporter ATP-binding protein -
  P5S46_RS17785 (P5S46_17785) - 3843393..3845018 (-) 1626 WP_277856309.1 iron ABC transporter permease -
  P5S46_RS17790 (P5S46_17790) - 3845058..3846065 (-) 1008 WP_039039971.1 Fe(3+) ABC transporter substrate-binding protein -
  P5S46_RS17795 (P5S46_17795) glnK 3846294..3846632 (-) 339 WP_005332325.1 P-II family nitrogen regulator -
  P5S46_RS17800 (P5S46_17800) arfB 3846834..3847250 (+) 417 WP_010673067.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  P5S46_RS17805 (P5S46_17805) - 3847277..3847771 (+) 495 WP_277856310.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  P5S46_RS17810 (P5S46_17810) - 3848373..3849569 (-) 1197 WP_041214468.1 NupC/NupG family nucleoside CNT transporter -
  P5S46_RS17815 (P5S46_17815) - 3849753..3850688 (-) 936 WP_010673064.1 LysR family transcriptional regulator -
  P5S46_RS17820 (P5S46_17820) - 3850771..3851307 (-) 537 WP_202044589.1 glutathione peroxidase -
  P5S46_RS17825 (P5S46_17825) - 3851367..3851930 (-) 564 WP_042014912.1 NnrU family protein -
  P5S46_RS17830 (P5S46_17830) - 3852193..3853326 (-) 1134 WP_277856311.1 glycerate kinase -
  P5S46_RS17835 (P5S46_17835) - 3853336..3854598 (-) 1263 WP_236276030.1 SLC13 family permease -
  P5S46_RS17840 (P5S46_17840) - 3854719..3855834 (-) 1116 WP_277856312.1 sugar diacid recognition domain-containing protein -
  P5S46_RS17845 (P5S46_17845) - 3856041..3856415 (+) 375 WP_039039964.1 YacL family protein -
  P5S46_RS17850 (P5S46_17850) - 3856465..3856755 (+) 291 WP_039039963.1 hypothetical protein -
  P5S46_RS17855 (P5S46_17855) - 3856917..3857747 (+) 831 WP_041214463.1 CPBP family intramembrane glutamic endopeptidase -
  P5S46_RS17860 (P5S46_17860) - 3857823..3858242 (-) 420 WP_041210940.1 DUF4426 domain-containing protein -
  P5S46_RS17865 (P5S46_17865) yggU 3858261..3858560 (-) 300 WP_277856313.1 DUF167 family protein YggU -
  P5S46_RS17870 (P5S46_17870) - 3858560..3859111 (-) 552 WP_010673053.1 YggT family protein -
  P5S46_RS17875 (P5S46_17875) proC 3859135..3859959 (-) 825 WP_010673052.1 pyrroline-5-carboxylate reductase -
  P5S46_RS17880 (P5S46_17880) - 3860082..3860783 (-) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  P5S46_RS17885 (P5S46_17885) pilT 3860823..3861857 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  P5S46_RS17890 (P5S46_17890) pilU 3861881..3862990 (+) 1110 WP_010673049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P5S46_RS17895 (P5S46_17895) yaaA 3863023..3863796 (+) 774 WP_103857984.1 peroxide stress protein YaaA -
  P5S46_RS17900 (P5S46_17900) srmB 3863984..3865204 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  P5S46_RS17905 (P5S46_17905) - 3865332..3866042 (+) 711 WP_183149273.1 methyltransferase -
  P5S46_RS17910 (P5S46_17910) brnQ 3866233..3867540 (+) 1308 WP_041211055.1 branched-chain amino acid transport system II carrier protein -
  P5S46_RS17915 (P5S46_17915) - 3867630..3867857 (-) 228 WP_039039956.1 hypothetical protein -
  P5S46_RS17920 (P5S46_17920) - 3867863..3868324 (-) 462 WP_010673043.1 chemotaxis protein CheX -
  P5S46_RS17925 (P5S46_17925) zur 3868314..3868772 (-) 459 WP_010673042.1 zinc uptake transcriptional repressor Zur -
  P5S46_RS17930 (P5S46_17930) - 3869353..3869616 (-) 264 WP_277856314.1 pyocin activator PrtN family protein -
  P5S46_RS17935 (P5S46_17935) - 3869667..3871349 (-) 1683 WP_277856315.1 DUF927 domain-containing protein -
  P5S46_RS17940 (P5S46_17940) - 3871928..3872275 (-) 348 WP_277856316.1 hypothetical protein -
  P5S46_RS17945 (P5S46_17945) - 3872365..3873515 (+) 1151 WP_111736006.1 IS3 family transposase -
  P5S46_RS17950 (P5S46_17950) - 3874667..3875776 (-) 1110 WP_045523182.1 caspase family protein -
  P5S46_RS17955 (P5S46_17955) - 3875792..3878275 (-) 2484 WP_277856317.1 effector-associated domain EAD1-containing protein -
  P5S46_RS17960 (P5S46_17960) - 3878272..3879132 (-) 861 WP_045523178.1 hypothetical protein -
  P5S46_RS17965 (P5S46_17965) - 3879129..3880172 (-) 1044 WP_338405877.1 hypothetical protein -
  P5S46_RS17970 (P5S46_17970) - 3880091..3881410 (-) 1320 WP_277856318.1 GTPase-associated system all-helical protein GASH -
  P5S46_RS17975 (P5S46_17975) - 3881666..3882766 (-) 1101 WP_045523172.1 site-specific integrase -
  P5S46_RS17980 (P5S46_17980) dusA 3882855..3883907 (+) 1053 WP_277856319.1 tRNA dihydrouridine(20/20a) synthase DusA -
  P5S46_RS17985 (P5S46_17985) - 3883910..3884059 (+) 150 WP_010673040.1 hypothetical protein -
  P5S46_RS17990 (P5S46_17990) - 3884142..3884567 (-) 426 WP_277856320.1 hypothetical protein -
  P5S46_RS17995 (P5S46_17995) hupA 3884681..3884953 (-) 273 WP_005305063.1 nucleoid-associated protein HU-alpha -
  P5S46_RS18000 (P5S46_18000) - 3885292..3885774 (+) 483 WP_029313757.1 Rsd/AlgQ family anti-sigma factor -
  P5S46_RS18005 (P5S46_18005) - 3885877..3886263 (+) 387 WP_029313755.1 PilZ domain-containing protein -
  P5S46_RS18010 (P5S46_18010) radA 3886317..3887681 (-) 1365 WP_277856321.1 DNA repair protein RadA -
  P5S46_RS18015 (P5S46_18015) - 3887782..3890157 (+) 2376 WP_277856322.1 PilZ domain-containing protein -
  P5S46_RS18020 (P5S46_18020) - 3890215..3891120 (-) 906 WP_042864464.1 alpha/beta fold hydrolase -
  P5S46_RS18025 (P5S46_18025) serB 3891149..3892153 (-) 1005 WP_277856323.1 phosphoserine phosphatase SerB -
  P5S46_RS18030 (P5S46_18030) - 3892347..3892955 (+) 609 WP_182936302.1 AhpA/YtjB family protein -
  P5S46_RS18035 (P5S46_18035) - 3893021..3895276 (+) 2256 WP_277856324.1 PAS domain S-box protein -
  P5S46_RS18040 (P5S46_18040) - 3895394..3896179 (-) 786 WP_233466745.1 RT0821/Lpp0805 family surface protein -
  P5S46_RS18045 (P5S46_18045) deoD 3896312..3897025 (-) 714 WP_039039949.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=809478 P5S46_RS17885 WP_010673050.1 3860823..3861857(+) (pilT) [Aeromonas caviae strain AC1520]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=809478 P5S46_RS17885 WP_010673050.1 3860823..3861857(+) (pilT) [Aeromonas caviae strain AC1520]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCATCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCCCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGACCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGCCTCG
CCATGACCGCCGCCGAAACCGGTCACCTGGTGTTTGGCACCCTGCACACCTCCTCGGCTGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAACTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGAGGGGTCGTCGCCTCCCTCGATGCCAAAGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407