Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TSUB_RS13585 Genome accession   NZ_AP023054
Coordinates   3050922..3051968 (-) Length   348 a.a.
NCBI ID   WP_087021974.1    Uniprot ID   -
Organism   Thaumasiovibrio subtropicus strain C4V358     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3045922..3056968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSUB_RS13570 ubiU 3047858..3048853 (+) 996 WP_087021965.1 ubiquinone anaerobic biosynthesis protein UbiU -
  TSUB_RS13575 - 3048872..3049735 (+) 864 WP_087021968.1 U32 family peptidase -
  TSUB_RS13580 pilU 3049791..3050909 (-) 1119 WP_087021971.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TSUB_RS13585 pilT 3050922..3051968 (-) 1047 WP_087021974.1 type IV pilus twitching motility protein PilT Machinery gene
  TSUB_RS13590 - 3051993..3052703 (+) 711 WP_087021977.1 YggS family pyridoxal phosphate-dependent enzyme -
  TSUB_RS13595 proC 3052753..3053571 (+) 819 WP_087021980.1 pyrroline-5-carboxylate reductase -
  TSUB_RS13600 - 3053600..3054157 (+) 558 WP_087021983.1 YggT family protein -
  TSUB_RS13605 yggU 3054154..3054447 (+) 294 WP_087021986.1 DUF167 family protein YggU -
  TSUB_RS13610 - 3054626..3055060 (+) 435 WP_087021989.1 DUF4426 domain-containing protein -
  TSUB_RS13615 - 3055161..3055751 (+) 591 WP_087021992.1 XTP/dITP diphosphatase -
  TSUB_RS13620 hemW 3055796..3056947 (+) 1152 WP_087021995.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38585.08 Da        Isoelectric Point: 6.5050

>NTDB_id=80851 TSUB_RS13585 WP_087021974.1 3050922..3051968(-) (pilT) [Thaumasiovibrio subtropicus strain C4V358]
MDITELLDFSVKHDASDLHLSAGVPPMIRVDGDVRKLSLPELTHAEVHKLIFDIMNDAQRREYEEKLECDFSFAVPNVGR
FRVNTFHQARGSAAVFRTIPEKIPTLDDIKAPEIFKNISELQKGLVLVTGPTGSGKSTTLAAMVNYINERYNRHILTIED
PIEFVHTSQKSLVNQREVHRDTHSFNAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGNDKAMVRSMLSESLRAVISQTLMKCTTGGRVAGHEIMLATPAIRNLIREDKVAQMYSMIQTGASQGMQTMEQAS
RMLVAQGLVEAEEARRKTAGPNSPQQQM

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=80851 TSUB_RS13585 WP_087021974.1 3050922..3051968(-) (pilT) [Thaumasiovibrio subtropicus strain C4V358]
ATGGATATCACCGAATTATTGGACTTTAGTGTAAAGCACGACGCCTCTGACTTGCATCTCTCTGCCGGGGTCCCCCCGAT
GATCCGTGTGGATGGCGATGTGCGAAAGCTCAGTCTACCAGAATTAACCCATGCTGAAGTGCACAAATTGATCTTTGATA
TCATGAATGATGCTCAGCGTCGGGAGTATGAAGAGAAACTTGAATGTGACTTCTCTTTTGCCGTACCTAATGTGGGTCGA
TTCCGTGTGAACACTTTCCACCAAGCACGCGGTAGCGCGGCGGTGTTCCGTACGATCCCTGAGAAGATTCCAACCTTGGA
TGACATCAAAGCACCAGAGATATTTAAAAATATCAGTGAGCTGCAAAAAGGTTTGGTGTTAGTGACCGGGCCAACGGGCT
CTGGTAAATCGACAACCTTGGCGGCGATGGTGAATTACATCAATGAACGTTATAACCGTCATATCCTCACCATTGAAGAC
CCGATTGAATTCGTCCATACCAGTCAGAAGAGCTTGGTTAACCAGCGTGAAGTACACCGTGATACGCACAGCTTTAACGC
GGCGCTACGTTCTGCGCTTCGTGAAGACCCGGATGTCATTCTGGTGGGTGAGCTTCGTGACCAAGAGACGATTAGCTTGG
CGTTGACTGCGGCGGAAACCGGTCACCTCGTTTTTGGTACTTTGCACACCAGTAGTGCAGCCAAAACGGTTGACCGTATC
ATTGATGTCTTCCCGGGTAACGATAAAGCCATGGTGCGCTCGATGCTATCGGAGTCATTACGCGCGGTGATTTCGCAAAC
CTTGATGAAGTGCACTACGGGCGGGCGTGTCGCGGGTCATGAGATTATGTTGGCAACACCGGCGATCCGTAACTTGATCC
GTGAGGACAAAGTCGCTCAGATGTACTCCATGATTCAAACCGGTGCGAGCCAAGGGATGCAGACGATGGAGCAAGCGTCG
CGGATGTTGGTTGCGCAAGGGCTGGTGGAAGCGGAAGAGGCGCGACGTAAGACGGCGGGGCCAAATAGTCCACAGCAACA
GATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.228

96.839

0.767
  pilT Vibrio cholerae strain A1552

79.228

96.839

0.767
  pilT Acinetobacter baumannii D1279779

70.845

98.563

0.698
  pilT Acinetobacter baumannii strain A118

70.845

98.563

0.698
  pilT Acinetobacter nosocomialis M2

70.554

98.563

0.695
  pilT Pseudomonas aeruginosa PAK

70.554

98.563

0.695
  pilT Acinetobacter baylyi ADP1

69.388

98.563

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.096

98.563

0.681
  pilT Legionella pneumophila strain Lp02

67.964

95.977

0.652
  pilT Legionella pneumophila strain ERS1305867

67.964

95.977

0.652
  pilT Neisseria meningitidis 8013

66.272

97.126

0.644
  pilT Neisseria gonorrhoeae MS11

65.976

97.126

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.258

91.379

0.468
  pilU Pseudomonas stutzeri DSM 10701

42.183

97.414

0.411
  pilU Vibrio cholerae strain A1552

40.896

96.264

0.394
  pilU Acinetobacter baylyi ADP1

38.571

100

0.388

Multiple sequence alignment