Detailed information NTG0080850

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: TSUB_RS13580
• Genome accession: NZ_AP023054
• Coordinates: 3049791..3050909 (-)
• Length: 372 a.a.
• NCBI ID: WP_087021971.1
• Uniprot ID: -
• Organism: Thaumasiovibrio subtropicus strain C4V358
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3044791..3055909

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TSUB_RS13565	-	3045354..3047393 (+)	2040	WP_087021963.1	EAL domain-containing protein	-
TSUB_RS13570	ubiU	3047858..3048853 (+)	996	WP_087021965.1	ubiquinone anaerobic biosynthesis protein UbiU	-
TSUB_RS13575	-	3048872..3049735 (+)	864	WP_087021968.1	U32 family peptidase	-
TSUB_RS13580	pilU	3049791..3050909 (-)	1119	WP_087021971.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
TSUB_RS13585	pilT	3050922..3051968 (-)	1047	WP_087021974.1	type IV pilus twitching motility protein PilT	Machinery gene
TSUB_RS13590	-	3051993..3052703 (+)	711	WP_087021977.1	YggS family pyridoxal phosphate-dependent enzyme	-
TSUB_RS13595	proC	3052753..3053571 (+)	819	WP_087021980.1	pyrroline-5-carboxylate reductase	-
TSUB_RS13600	-	3053600..3054157 (+)	558	WP_087021983.1	YggT family protein	-
TSUB_RS13605	yggU	3054154..3054447 (+)	294	WP_087021986.1	DUF167 family protein YggU	-
TSUB_RS13610	-	3054626..3055060 (+)	435	WP_087021989.1	DUF4426 domain-containing protein	-
TSUB_RS13615	-	3055161..3055751 (+)	591	WP_087021992.1	XTP/dITP diphosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 42073.24 Da
• Isoelectric Point: 6.0572

>NTDB_id=80850 TSUB_RS13580 WP_087021971.1 3049791..3050909(-) (pilU) [Thaumasiovibrio subtropicus strain C4V358]
MQINQVLNEMVSVKASDLYITVGAPALLKVNGRLNPYGDELSQAAVNIMLNSVMDDDKRQEFFDSREANFAIVRDCGRFR
VSAFWQRDMPGMVIRRIETHIPQFQELSLPREMEHMVMSKRGLILVVGATGSGKSTSMAAMTGHRNRNASGHILTIEDPI
EFVHDHDNCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMMFAETGHLCMATLHANNANQALERILH
LVPKERREQFLFDLSLNLKCVLAQQLVPDINGKGRHGAFELLINTPRVSDLIRRAELHELKEVMRRGKEQGMRTFDQALY
QLVTDSKISELEAMHHADSENELRLMIKLGSSDDKPLDGSGSSLKDVKIEMD

Nucleotide

• Length: 1119 bp

>NTDB_id=80850 TSUB_RS13580 WP_087021971.1 3049791..3050909(-) (pilU) [Thaumasiovibrio subtropicus strain C4V358]
ATGCAGATTAATCAGGTTTTGAACGAAATGGTCAGTGTAAAGGCATCCGACCTTTACATTACTGTGGGGGCACCTGCGTT
GCTCAAAGTGAACGGTCGACTCAACCCTTATGGCGATGAGCTGTCTCAAGCGGCGGTCAACATCATGCTGAATAGCGTGA
TGGATGATGATAAGCGACAAGAGTTTTTTGATAGTCGTGAGGCGAACTTTGCGATCGTGCGCGATTGTGGACGTTTTCGT
GTTAGCGCGTTTTGGCAGCGTGATATGCCGGGGATGGTCATTCGTCGTATCGAAACTCATATTCCACAGTTCCAAGAACT
TTCGTTACCGCGAGAGATGGAACATATGGTGATGTCGAAACGTGGTCTGATTCTGGTTGTTGGGGCGACAGGCTCGGGTA
AGTCGACCTCGATGGCGGCGATGACAGGGCATCGAAACCGCAATGCCAGTGGTCACATTTTGACAATCGAAGACCCGATC
GAATTCGTACATGACCATGACAACTGTATTGTCACGCAGCGAGAGGTAGGACTCGATACGGAGAGTTATGAAGTCGCGCT
GAAGAACTCTTTACGTCAAGCGCCAGACATGATTCTGATCGGTGAGATCCGTACCCGAGAGACGATGGAATACGCGATGA
TGTTCGCTGAAACGGGCCATTTGTGTATGGCGACGTTGCACGCCAACAACGCTAACCAAGCCTTAGAGCGTATTTTGCAT
CTAGTGCCTAAAGAACGTCGTGAGCAGTTCTTGTTTGATCTCTCTTTAAATCTTAAATGTGTATTGGCGCAGCAGTTGGT
GCCTGATATTAATGGTAAAGGGCGTCATGGGGCGTTTGAGCTGCTGATCAATACCCCGCGGGTTTCTGATTTGATTCGCC
GCGCTGAGTTGCATGAACTAAAAGAAGTGATGCGACGGGGTAAAGAGCAAGGGATGCGAACCTTCGACCAAGCGCTCTAT
CAGCTGGTGACAGACAGTAAGATCTCGGAACTGGAAGCGATGCACCATGCGGATTCTGAAAATGAATTGCGCTTGATGAT
CAAGCTCGGCAGCAGTGACGACAAGCCACTAGATGGCAGTGGTAGCAGTCTTAAAGATGTGAAAATCGAGATGGATTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (44-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	70.968	100	0.71
pilU	Pseudomonas stutzeri DSM 10701	58.708	95.699	0.562
pilU	Acinetobacter baylyi ADP1	56.284	98.387	0.554
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.975	87.097	0.366