Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P6988_RS04870 Genome accession   NZ_CP120719
Coordinates   1008091..1009197 (+) Length   368 a.a.
NCBI ID   WP_277684946.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain Rb102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1003091..1014197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6988_RS04835 (P6988_04835) - 1003297..1003899 (-) 603 WP_006958269.1 XTP/dITP diphosphatase -
  P6988_RS04840 (P6988_04840) - 1003939..1004370 (-) 432 WP_019277255.1 DUF4426 domain-containing protein -
  P6988_RS04845 (P6988_04845) yggU 1004569..1004859 (-) 291 WP_277684702.1 DUF167 family protein YggU -
  P6988_RS04850 (P6988_04850) - 1004859..1005416 (-) 558 WP_006958264.1 YggT family protein -
  P6988_RS04855 (P6988_04855) proC 1005455..1006273 (-) 819 WP_277684703.1 pyrroline-5-carboxylate reductase -
  P6988_RS04860 (P6988_04860) - 1006301..1007017 (-) 717 WP_172858254.1 YggS family pyridoxal phosphate-dependent enzyme -
  P6988_RS04865 (P6988_04865) pilT 1007042..1008079 (+) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  P6988_RS04870 (P6988_04870) pilU 1008091..1009197 (+) 1107 WP_277684946.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P6988_RS04875 (P6988_04875) ruvX 1009307..1009729 (-) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  P6988_RS04880 (P6988_04880) - 1009747..1010310 (-) 564 WP_019277262.1 YqgE/AlgH family protein -
  P6988_RS04885 (P6988_04885) gshB 1010367..1011317 (-) 951 WP_019277263.1 glutathione synthase -
  P6988_RS04890 (P6988_04890) rsmE 1011331..1012062 (-) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P6988_RS04895 (P6988_04895) - 1012171..1012875 (-) 705 WP_065741130.1 endonuclease -
  P6988_RS04900 (P6988_04900) - 1012955..1013452 (-) 498 WP_021458543.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41219.86 Da        Isoelectric Point: 5.3077

>NTDB_id=808301 P6988_RS04870 WP_277684946.1 1008091..1009197(+) (pilU) [Vibrio coralliilyticus strain Rb102]
MDINPYLQGMLAEKASDLYITVGAPILYRVDGELKPHGESLSEAEVLTLLHSTMDEERQNDYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSQEIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=808301 P6988_RS04870 WP_277684946.1 1008091..1009197(+) (pilU) [Vibrio coralliilyticus strain Rb102]
ATGGATATCAATCCTTATCTGCAAGGCATGCTGGCTGAGAAAGCGTCAGACTTATACATCACTGTTGGTGCGCCGATACT
TTATCGTGTTGATGGTGAGTTGAAGCCGCATGGAGAGAGCTTATCGGAAGCCGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAATGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAGTGCGGGATTTTGGTCGTTTTCGT
GTTTCTGCTTTCTATCAGCGAGAATTACCCGGTGCGGTGATTCGTCGAATTGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCTGAAACTCTGAAAGATCTTTCCATCGCCAAGCGCGGCTTAGTATTAGTAGTGGGCGCCACAGGTTCCGGTA
AATCCACAACAATGGCAGCGATGACAGGCTATCGAAATACTGAGCGCAGCGGCCATATTCTGACAGTTGAAGACCCGATT
GAGTTTGTCCACGAGCATCAGAAGTGCATCGTCACGCAACGTGAGGTTGGCTTAGACACAGAAAGTTATGAAGTCGCGCT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATCTTGATTGGTGAGATCCGCTCGCGAGAGACCATGGAATACGCGATGA
CATTCGCCGAAACGGGGCATCTGTGTATGGCGACGTTGCATGCTAATAATGCAAACCAAGCTTTGGAAAGGATTCTGCAC
CTGGTACCGAAAGAGCAAAAAGAGCAGTTCTTGTTTGATCTCTCAATGAACCTCAAAGGTGTTATCGGTCAGCAGTTGAT
TCGAGATAAAAACGGCCAAGGTCGTCATGGCGTTTTTGAAATTCTACTTAACAGTCCACGAGTATCGGATCTCATTCGAC
GCGGTGATCTGCATGAACTCAAATCAACCATGGCGAAATCTCAGGAAATTGGTATGCAGACGTTTGATCAGGCGCTTTAC
CAACTGGTGGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCAGATTCAGCCAATGATCTGCGTATCATGCT
TAAAACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

52.991

95.38

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

92.12

0.408
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.231

88.315

0.364
  pilT Vibrio cholerae strain A1552

41.231

88.315

0.364