Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P6988_RS04865 Genome accession   NZ_CP120719
Coordinates   1007042..1008079 (+) Length   345 a.a.
NCBI ID   WP_006958261.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain Rb102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1002042..1013079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6988_RS04830 (P6988_04830) hemW 1002119..1003297 (-) 1179 WP_277684701.1 radical SAM family heme chaperone HemW -
  P6988_RS04835 (P6988_04835) - 1003297..1003899 (-) 603 WP_006958269.1 XTP/dITP diphosphatase -
  P6988_RS04840 (P6988_04840) - 1003939..1004370 (-) 432 WP_019277255.1 DUF4426 domain-containing protein -
  P6988_RS04845 (P6988_04845) yggU 1004569..1004859 (-) 291 WP_277684702.1 DUF167 family protein YggU -
  P6988_RS04850 (P6988_04850) - 1004859..1005416 (-) 558 WP_006958264.1 YggT family protein -
  P6988_RS04855 (P6988_04855) proC 1005455..1006273 (-) 819 WP_277684703.1 pyrroline-5-carboxylate reductase -
  P6988_RS04860 (P6988_04860) - 1006301..1007017 (-) 717 WP_172858254.1 YggS family pyridoxal phosphate-dependent enzyme -
  P6988_RS04865 (P6988_04865) pilT 1007042..1008079 (+) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  P6988_RS04870 (P6988_04870) pilU 1008091..1009197 (+) 1107 WP_277684946.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P6988_RS04875 (P6988_04875) ruvX 1009307..1009729 (-) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  P6988_RS04880 (P6988_04880) - 1009747..1010310 (-) 564 WP_019277262.1 YqgE/AlgH family protein -
  P6988_RS04885 (P6988_04885) gshB 1010367..1011317 (-) 951 WP_019277263.1 glutathione synthase -
  P6988_RS04890 (P6988_04890) rsmE 1011331..1012062 (-) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P6988_RS04895 (P6988_04895) - 1012171..1012875 (-) 705 WP_065741130.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38121.49 Da        Isoelectric Point: 6.1894

>NTDB_id=808300 P6988_RS04865 WP_006958261.1 1007042..1008079(+) (pilT) [Vibrio coralliilyticus strain Rb102]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFEIMNDAQRSEFEERLEVDFSFALPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLEAPSIFENIANMEKGLILVTGPTGSGKSTTLAAMVDHINRHHNKHILTIED
PIEFVHTNNKCLINQREVHNDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=808300 P6988_RS04865 WP_006958261.1 1007042..1008079(+) (pilT) [Vibrio coralliilyticus strain Rb102]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCGTTCAACCATTCCGAAGTGCATCGTTTGGTGTTTGAAA
TTATGAACGATGCTCAGCGTAGCGAATTTGAAGAACGTCTTGAAGTCGACTTTTCTTTTGCACTTCCGGACGTTGGCCGT
TTTAGGGTCAACGCTTTTAACCAATCTCGTGGTTGTGCGGCCGTATTTCGTACCATTCCAACCGATATCCCGACTTTAGA
GCAGCTAGAAGCGCCGTCGATCTTTGAAAATATCGCCAATATGGAGAAAGGATTAATTTTGGTGACAGGCCCGACGGGCT
CAGGTAAATCGACCACTCTGGCGGCAATGGTGGATCATATTAATCGTCACCACAATAAGCATATCCTGACTATTGAAGAT
CCGATTGAGTTTGTGCACACCAATAACAAATGTCTGATCAACCAGCGTGAAGTTCACAATGATACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCATTGCGTGAAGATCCCGATGTCATTTTAGTTGGTGAGCTACGCGATCAGGAAACGATCAGTCTAG
CGCTGACCGCTGCTGAAACCGGCCATTTGGTGTTTGGTACTTTGCACACCAGTTCGGCGGCAAAAACTATCGACCGTATT
ATTGATGTTTTCCCAGGTAGCGACAAAGGTATGGTACGTTCCATGCTCTCTGAATCTCTGCGTGCGGTGATTGCGCAGAA
ACTACTCAAACGAATTGGCGGCGGGAGAACTGCTTGTCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAAATGCAGTCTGTGATTCAGACTGGTGCGGCGCACGGTATGCAAACCATGGAGCAGCACTCA
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAAAAAATCGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

100

0.872
  pilT Vibrio cholerae strain A1552

87.246

100

0.872
  pilT Acinetobacter baylyi ADP1

68.713

99.13

0.681
  pilT Acinetobacter baumannii strain A118

69.277

96.232

0.667
  pilT Acinetobacter baumannii D1279779

69.277

96.232

0.667
  pilT Acinetobacter nosocomialis M2

68.976

96.232

0.664
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.681

0.652
  pilT Pseudomonas aeruginosa PAK

65.497

99.13

0.649
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.462

94.203

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

41.25

92.754

0.383