Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P6910_RS02275 Genome accession   NZ_CP120717
Coordinates   574095..575129 (+) Length   344 a.a.
NCBI ID   WP_317144671.1    Uniprot ID   -
Organism   Endozoicomonas sp. 8E     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569095..580129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6910_RS02255 (P6910_02250) - 569635..571575 (+) 1941 WP_317144667.1 hypothetical protein -
  P6910_RS02260 (P6910_02255) - 571738..572289 (-) 552 WP_317144668.1 YggT family protein -
  P6910_RS02265 (P6910_02260) proC 572459..573283 (-) 825 WP_317144669.1 pyrroline-5-carboxylate reductase -
  P6910_RS02270 (P6910_02265) - 573273..573989 (-) 717 WP_317144670.1 YggS family pyridoxal phosphate-dependent enzyme -
  P6910_RS02275 (P6910_02270) pilT 574095..575129 (+) 1035 WP_317144671.1 type IV pilus twitching motility protein PilT Machinery gene
  P6910_RS02280 (P6910_02275) pilU 575132..576259 (+) 1128 WP_317144672.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P6910_RS02285 (P6910_02280) - 576290..577150 (-) 861 WP_317144673.1 hypothetical protein -
  P6910_RS02290 (P6910_02285) ruvX 577128..577568 (-) 441 WP_317144674.1 Holliday junction resolvase RuvX -
  P6910_RS02295 (P6910_02290) - 577612..578232 (-) 621 WP_317144675.1 YqgE/AlgH family protein -
  P6910_RS02300 (P6910_02295) - 578253..579119 (-) 867 WP_317144676.1 energy transducer TonB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38056.73 Da        Isoelectric Point: 6.7808

>NTDB_id=808233 P6910_RS02275 WP_317144671.1 574095..575129(+) (pilT) [Endozoicomonas sp. 8E]
MDITELLAFSHKQGASDLHISAGLPPMIRIDGDIRRINLPPMDHKQVHSLIYDVMNDKQRKDYEEKLETDFSFEVPGVAR
FRVNAFNQSRGAGAVFRTIPSKVLSMEDLGMGQVFKDIALKPRGLVLVTGPTGSGKSTSLAAMMDYINDNKYEHILTIED
PIEFVHASKKCLVNQREVHRDTHSFSDALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGAEKAMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMIGTPAIRNLIREDKIAQMYSSIQTGGALGMQTLDQCL
HELVRKGLIAKDVAQEKARDPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=808233 P6910_RS02275 WP_317144671.1 574095..575129(+) (pilT) [Endozoicomonas sp. 8E]
ATGGACATAACCGAACTGCTGGCCTTCAGTCACAAGCAAGGCGCTTCGGATCTTCACATCTCAGCCGGCCTTCCGCCCAT
GATTCGTATAGACGGTGATATTCGCCGGATTAACCTGCCTCCCATGGATCACAAGCAAGTTCACAGCCTGATCTATGACG
TCATGAACGACAAGCAGCGAAAGGACTACGAAGAGAAGCTGGAAACCGACTTTTCGTTTGAGGTCCCCGGTGTCGCCCGC
TTTCGTGTCAACGCTTTTAACCAGAGCCGCGGAGCCGGTGCTGTATTTCGTACTATCCCCAGTAAAGTGCTTTCGATGGA
AGACCTGGGCATGGGCCAGGTGTTCAAGGATATTGCCCTGAAGCCCCGGGGTCTGGTGCTGGTGACGGGTCCTACAGGTT
CCGGAAAGAGTACCAGTCTGGCGGCCATGATGGATTACATCAATGACAACAAGTATGAGCATATACTAACCATCGAAGAC
CCCATTGAATTTGTTCACGCCTCGAAAAAATGTCTGGTAAACCAGCGGGAAGTTCACAGGGATACCCACAGTTTCAGCGA
CGCACTGCGCTCGGCACTTCGTGAAGACCCCGATATTATCCTGGTGGGTGAGATGCGCGATCTTGAAACCATTCGTCTTG
CGCTGACGGCTGCAGAAACCGGTCACCTGGTTTTCGGGACTCTGCATACCACCTCCGCAGCCAAGACGATAGACCGTGTG
GTGGACGTATTCCCGGGAGCTGAAAAAGCTATGGTGCGCTCCATGCTTTCAGAATCCCTGCAAGCAGTTATCTCCCAGAC
CCTGCTCAAGAAAAACGGCGGCGGACGTGTGGCAGCCCACGAGATTATGATCGGTACTCCTGCTATTCGTAACCTGATCC
GCGAAGACAAGATTGCCCAGATGTACTCTTCCATCCAGACCGGTGGTGCCCTGGGTATGCAGACACTTGACCAGTGTCTC
CACGAGCTGGTGAGAAAAGGCTTGATTGCCAAAGATGTGGCTCAGGAAAAAGCCCGGGACCCGGAAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.919

100

0.869
  pilT Pseudomonas aeruginosa PAK

86.047

100

0.86
  pilT Acinetobacter nosocomialis M2

81.395

100

0.814
  pilT Acinetobacter baumannii D1279779

81.395

100

0.814
  pilT Acinetobacter baumannii strain A118

81.395

100

0.814
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

75.872

100

0.759
  pilT Legionella pneumophila strain ERS1305867

75.872

100

0.759
  pilT Neisseria meningitidis 8013

68.406

100

0.686
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.384

0.416
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

41.64

92.151

0.384