Detailed information NTG0808234

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P6910_RS02280
• Genome accession: NZ_CP120717
• Coordinates: 575132..576259 (+)
• Length: 375 a.a.
• NCBI ID: WP_317144672.1
• Uniprot ID: A0AA97IJ02
• Organism: Endozoicomonas sp. 8E
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 570132..581259

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P6910_RS02260 (P6910_02255)	-	571738..572289 (-)	552	WP_317144668.1	YggT family protein	-
P6910_RS02265 (P6910_02260)	proC	572459..573283 (-)	825	WP_317144669.1	pyrroline-5-carboxylate reductase	-
P6910_RS02270 (P6910_02265)	-	573273..573989 (-)	717	WP_317144670.1	YggS family pyridoxal phosphate-dependent enzyme	-
P6910_RS02275 (P6910_02270)	pilT	574095..575129 (+)	1035	WP_317144671.1	type IV pilus twitching motility protein PilT	Machinery gene
P6910_RS02280 (P6910_02275)	pilU	575132..576259 (+)	1128	WP_317144672.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P6910_RS02285 (P6910_02280)	-	576290..577150 (-)	861	WP_317144673.1	hypothetical protein	-
P6910_RS02290 (P6910_02285)	ruvX	577128..577568 (-)	441	WP_317144674.1	Holliday junction resolvase RuvX	-
P6910_RS02295 (P6910_02290)	-	577612..578232 (-)	621	WP_317144675.1	YqgE/AlgH family protein	-
P6910_RS02300 (P6910_02295)	-	578253..579119 (-)	867	WP_317144676.1	energy transducer TonB	-
P6910_RS02305 (P6910_02300)	-	579370..580731 (+)	1362	WP_317144677.1	AAA family ATPase	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 42241.65 Da
• Isoelectric Point: 7.0770

>NTDB_id=808234 P6910_RS02280 WP_317144672.1 575132..576259(+) (pilU) [Endozoicomonas sp. 8E]
MDINRLLKVMAEKKASDLFITAGFPPSFKLNGRIVPVTREPLTPAMTRDMVYGVMDEDQKAEFNREHECNFAISASGIGR
FRVSAFYQRNLAGMVLRRIETEIPTLDQLKLPKVIKELSMSRRGLVLFVGATGVGKSTSLAAMLGHRNEHACGHIISIED
PIEFIHQHRKSIVTQREVGIDTESYAVALKNTLRQAPDVIMIGEIRTRETMEYALAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQIWMDLSLNLKGLVAQQLVRTRDGKDRRAAVEVLLNTPLIQDTILHGNVHELKTIMKKSHELGMQTFDQA
LFELYNNGEITYEEALSHADSPNDLRLMIKLGPKTDGEHLTKTADKLSLQHEEIY

Nucleotide

• Length: 1128 bp

>NTDB_id=808234 P6910_RS02280 WP_317144672.1 575132..576259(+) (pilU) [Endozoicomonas sp. 8E]
ATGGATATCAATCGTTTACTAAAAGTGATGGCCGAAAAGAAGGCCTCTGACCTCTTTATCACGGCTGGCTTTCCACCCAG
TTTTAAACTCAATGGCAGGATTGTGCCTGTCACCAGGGAGCCTCTGACACCTGCCATGACCCGGGACATGGTCTACGGTG
TGATGGATGAAGATCAAAAAGCCGAATTTAATCGCGAGCATGAATGCAACTTTGCAATATCAGCCTCTGGAATAGGCCGC
TTTCGTGTCAGTGCTTTCTACCAGCGAAATTTGGCTGGCATGGTATTGAGAAGGATTGAGACCGAGATCCCGACGCTGGA
TCAGCTGAAGTTGCCGAAGGTGATTAAAGAGCTTTCCATGAGCAGAAGAGGTCTTGTGCTTTTTGTTGGGGCCACCGGTG
TTGGTAAATCGACTTCCCTTGCGGCGATGCTCGGGCATCGGAATGAACATGCCTGCGGGCACATCATCAGTATTGAAGAC
CCGATTGAATTTATCCATCAGCATCGCAAATCCATTGTCACCCAGCGTGAAGTAGGCATTGATACAGAGTCCTATGCCGT
GGCTCTGAAAAACACCCTGCGACAGGCACCGGATGTCATCATGATTGGTGAAATCAGAACCCGTGAAACAATGGAATATG
CCCTGGCATTTGCGGAGACAGGGCATCTCTGTCTGGCGACACTGCACGCAAATAATGCTAACCAGGCACTGGAAAGGATT
ATCCATTTCTTCCCGGCGGATCGTCATGGTCAGATATGGATGGACTTATCCCTGAACCTGAAAGGCCTGGTTGCCCAGCA
GCTGGTGAGAACCCGGGATGGCAAAGATCGCCGGGCAGCCGTTGAGGTTCTTCTCAATACACCCTTGATTCAGGATACTA
TCCTTCACGGTAATGTTCATGAACTCAAGACCATTATGAAGAAGTCTCATGAGCTGGGTATGCAAACCTTTGATCAGGCG
TTGTTTGAGCTTTATAACAATGGAGAAATCACTTACGAAGAAGCTCTGTCGCATGCGGATTCACCTAACGATCTGCGTCT
CATGATCAAGCTGGGGCCAAAGACTGATGGGGAGCATCTGACCAAAACGGCAGACAAGCTCAGTTTACAGCATGAGGAGA
TCTACTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	72.973	98.667	0.72
pilU	Acinetobacter baylyi ADP1	63.585	95.2	0.605
pilU	Vibrio cholerae strain A1552	58.286	93.333	0.544
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.548	97.333	0.395