Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVE91_RS04305 Genome accession   NZ_CP120400
Coordinates   923161..924198 (+) Length   345 a.a.
NCBI ID   WP_053519259.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain LH-B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 918161..929198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE91_RS04280 soxR 919441..919875 (+) 435 WP_053519250.1 redox-sensitive transcriptional activator SoxR -
  PVE91_RS04285 - 919917..920519 (-) 603 WP_074900439.1 short chain dehydrogenase -
  PVE91_RS04290 - 920620..921495 (+) 876 WP_111187135.1 LysR family transcriptional regulator -
  PVE91_RS04295 proC 921556..922377 (-) 822 WP_277381461.1 pyrroline-5-carboxylate reductase -
  PVE91_RS04300 - 922396..923073 (-) 678 WP_128989763.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVE91_RS04305 pilT 923161..924198 (+) 1038 WP_053519259.1 type IV pilus twitching motility protein PilT Machinery gene
  PVE91_RS04310 pilU 924346..925440 (+) 1095 WP_053519270.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVE91_RS04315 - 925598..926581 (+) 984 WP_065175975.1 aldo/keto reductase -
  PVE91_RS04320 - 926612..927514 (-) 903 WP_053519263.1 LysR family transcriptional regulator -
  PVE91_RS04325 - 927673..928380 (+) 708 WP_053519265.1 YitT family protein -
  PVE91_RS04330 - 928630..929175 (-) 546 WP_053519397.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38610.37 Da        Isoelectric Point: 6.6654

>NTDB_id=805496 PVE91_RS04305 WP_053519259.1 923161..924198(+) (pilT) [Stenotrophomonas maltophilia strain LH-B2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPIFRELIQQPQGLILVTGPTGSGKSTTLAAMIDYINKSEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQQGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=805496 PVE91_RS04305 WP_053519259.1 923161..924198(+) (pilT) [Stenotrophomonas maltophilia strain LH-B2]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGCATCAACATCCCGGCACTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAAGTGGACTTCTCGTTCGAGATCCCGTCGCTGGCACGT
TTCCGCGTGAATGCGTTCAACCAGAACCGGGGTGCGGGCGCGGTGTTCCGTACCATTCCGTCGGAGGTTCTGACGCTGGA
GGATCTGGCCTGCCCGCCGATCTTCCGCGAACTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTACATCAACAAGAGCGAATACGGCCACATCCTCACTGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTACACCGCGATACGCATGGCTTCAACGA
GGCACTGCGCTCGGCACTGCGAGAAGACCCGGACATCATCCTGGTCGGCGAACTGCGCGACCTGGAAACCATCCGTCTGG
CACTGACCGCCGCGGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCTCGCAGGC
ACTGTTGAAGAAGGTGGGCGGCGGCCGTACCGCAGCGTGGGAGATCATGGTGGGCACGCCGGCCATCCGCAATCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGTCAGCAGCAGGGCATGATGACGCTCGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGACTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397