Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVE91_RS04310 Genome accession   NZ_CP120400
Coordinates   924346..925440 (+) Length   364 a.a.
NCBI ID   WP_053519270.1    Uniprot ID   A0AAP7L0F7
Organism   Stenotrophomonas maltophilia strain LH-B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 919346..930440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE91_RS04280 soxR 919441..919875 (+) 435 WP_053519250.1 redox-sensitive transcriptional activator SoxR -
  PVE91_RS04285 - 919917..920519 (-) 603 WP_074900439.1 short chain dehydrogenase -
  PVE91_RS04290 - 920620..921495 (+) 876 WP_111187135.1 LysR family transcriptional regulator -
  PVE91_RS04295 proC 921556..922377 (-) 822 WP_277381461.1 pyrroline-5-carboxylate reductase -
  PVE91_RS04300 - 922396..923073 (-) 678 WP_128989763.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVE91_RS04305 pilT 923161..924198 (+) 1038 WP_053519259.1 type IV pilus twitching motility protein PilT Machinery gene
  PVE91_RS04310 pilU 924346..925440 (+) 1095 WP_053519270.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVE91_RS04315 - 925598..926581 (+) 984 WP_065175975.1 aldo/keto reductase -
  PVE91_RS04320 - 926612..927514 (-) 903 WP_053519263.1 LysR family transcriptional regulator -
  PVE91_RS04325 - 927673..928380 (+) 708 WP_053519265.1 YitT family protein -
  PVE91_RS04330 - 928630..929175 (-) 546 WP_053519397.1 DNA-3-methyladenine glycosylase I -
  PVE91_RS04335 - 929265..929831 (+) 567 WP_065182436.1 YqgE/AlgH family protein -
  PVE91_RS04340 ruvX 929824..930291 (+) 468 WP_053519401.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40549.61 Da        Isoelectric Point: 6.7716

>NTDB_id=805497 PVE91_RS04310 WP_053519270.1 924346..925440(+) (pilU) [Stenotrophomonas maltophilia strain LH-B2]
MAHQRASDLFITAGMPPAMKVNGKIAPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLATLHANNANQAMDRIINFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=805497 PVE91_RS04310 WP_053519270.1 924346..925440(+) (pilU) [Stenotrophomonas maltophilia strain LH-B2]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACCGCGGGCATGCCGCCGGCGATGAAGGTCAATGGCAAGATCGCGCC
GATCACGCAGACACCCCTGACGCCGCAGCAGAGCCGCGACCTGGTGCTGAACGTGATGACGCCGGCACAGCGCGAAGAGT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTGAGCTGCTTCTATCAGCGC
AACCAGGTGGGCATGGTGCTGCGTCGCATCGAGACGCGCATCCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCGACCGGTACCGGCAAGTCCACGTCGCTGGCAGCGA
TGATCGGTTATCGCAACCAGAATTCCACCGGCCACATCATCACCATCGAGGATCCGATCGAGTTCGTGCACAAGCACGAG
GGCTGTATCATCACCCAGCGCGAAGTGGGCATCGATACCGACAGCTGGGAGGCCGCGCTGAAGAACACGCTGCGCCAGGC
GCCGGACGTGATCATGATTGGTGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACC
TGGTGCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGGTGCCGTCCCCGGATGGCCGCTC
GCGCAAGGTTGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGAATCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAG
ATCAGCTACGAGGATGCGCTGCGGTATGCCGATTCGCAGAATGAAGTGCGGCTGCGCATCAAGCTGAGCCAGGGCGGTGA
CGCGCGCACCCTGTCGCAGGGGCTGGACGGCGTTGAAATCTCCGAGATCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.274

98.352

0.681
  pilU Acinetobacter baylyi ADP1

64.655

95.604

0.618
  pilU Vibrio cholerae strain A1552

54.755

95.33

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.609

94.78

0.404
  pilT Acinetobacter baumannii strain A118

40.606

90.659

0.368
  pilT Acinetobacter nosocomialis M2

40.606

90.659

0.368
  pilT Acinetobacter baumannii D1279779

40.606

90.659

0.368
  pilT Legionella pneumophila strain ERS1305867

40.867

88.736

0.363
  pilT Legionella pneumophila strain Lp02

40.867

88.736

0.363