Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P3T65_RS07955 Genome accession   NZ_CP120376
Coordinates   1711907..1712941 (+) Length   344 a.a.
NCBI ID   WP_024766758.1    Uniprot ID   -
Organism   Pseudomonas nitroreducens strain L4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1706907..1717941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T65_RS07930 (P3T65_07930) - 1707262..1709229 (-) 1968 WP_277369188.1 dynamin-like GTPase family protein -
  P3T65_RS07935 (P3T65_07935) - 1709348..1709638 (-) 291 WP_024766762.1 DUF167 domain-containing protein -
  P3T65_RS07940 (P3T65_07940) - 1709689..1710282 (-) 594 WP_024766761.1 YggT family protein -
  P3T65_RS07945 (P3T65_07945) proC 1710296..1711126 (-) 831 WP_024766760.1 pyrroline-5-carboxylate reductase -
  P3T65_RS07950 (P3T65_07950) - 1711144..1711833 (-) 690 WP_037013506.1 YggS family pyridoxal phosphate-dependent enzyme -
  P3T65_RS07955 (P3T65_07955) pilT 1711907..1712941 (+) 1035 WP_024766758.1 type IV pilus twitching motility protein PilT Machinery gene
  P3T65_RS07960 (P3T65_07960) pilU 1713046..1714191 (+) 1146 WP_170858008.1 type IV pilus ATPase PilU Machinery gene
  P3T65_RS07965 (P3T65_07965) - 1714266..1714670 (+) 405 WP_024766756.1 NINE protein -
  P3T65_RS07970 (P3T65_07970) - 1714895..1716268 (+) 1374 WP_277369189.1 cystathionine beta-synthase -
  P3T65_RS07975 (P3T65_07975) - 1716265..1717434 (+) 1170 WP_277369190.1 trans-sulfuration enzyme family protein -
  P3T65_RS07980 (P3T65_07980) - 1717524..1717895 (-) 372 WP_277369191.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37900.73 Da        Isoelectric Point: 6.7751

>NTDB_id=805260 P3T65_RS07955 WP_024766758.1 1711907..1712941(+) (pilT) [Pseudomonas nitroreducens strain L4]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFKRVSDVPRGLVLVTGPTGSGKSTSLAAMLDYLNNTKYSHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KGLVAKGLVTRDSAKEKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=805260 P3T65_RS07955 WP_024766758.1 1711907..1712941(+) (pilT) [Pseudomonas nitroreducens strain L4]
ATGGATATTACCGAGCTGCTCGCCTTCAGCGCCAAGCAGGGCGCTTCGGACCTGCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTGCGCCGCATCAACCTGCCGCCGCTCGAGCACAAGCAGGTTCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGTAAGGATTACGAAGAGTTCCTGGAAACCGACTTTTCCTTCGAAGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTACTCACGATGGA
GGACCTGGGAATGGGAGAAGTGTTCAAACGTGTTTCAGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCCACCTCGCTGGCGGCGATGCTCGACTACCTGAACAACACCAAGTACAGCCATATTCTCACTGTCGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCTGGGCTTCAACGA
GGCACTGCGCTCGGCCCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTCTTCGGCACCCTGCACACTACCTCCGCGGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCTGCCGAGGAGAAGTCCATGGTCCGCTCCATGCTCTCCGAGTCCCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGATCGGTGGCGGCCGTGTCGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GTGAGGACAAGGTTGCGCAGATGTACTCCGCCATCCAGACCGGCGGCGCGCTCGGCATGCAGACCCTGGACATGTGCCTG
AAAGGCCTGGTCGCCAAGGGCCTAGTCACCCGCGATTCGGCGAAAGAAAAAGCCAAGATCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

95.349

100

0.953
  pilT Pseudomonas stutzeri DSM 10701

93.605

100

0.936
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

76.331

98.256

0.75
  pilT Legionella pneumophila strain ERS1305867

76.331

98.256

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392