Detailed information NTG0805261

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P3T65_RS07960
• Genome accession: NZ_CP120376
• Coordinates: 1713046..1714191 (+)
• Length: 381 a.a.
• NCBI ID: WP_170858008.1
• Uniprot ID: -
• Organism: Pseudomonas nitroreducens strain L4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1708046..1719191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3T65_RS07935 (P3T65_07935)	-	1709348..1709638 (-)	291	WP_024766762.1	DUF167 domain-containing protein	-
P3T65_RS07940 (P3T65_07940)	-	1709689..1710282 (-)	594	WP_024766761.1	YggT family protein	-
P3T65_RS07945 (P3T65_07945)	proC	1710296..1711126 (-)	831	WP_024766760.1	pyrroline-5-carboxylate reductase	-
P3T65_RS07950 (P3T65_07950)	-	1711144..1711833 (-)	690	WP_037013506.1	YggS family pyridoxal phosphate-dependent enzyme	-
P3T65_RS07955 (P3T65_07955)	pilT	1711907..1712941 (+)	1035	WP_024766758.1	type IV pilus twitching motility protein PilT	Machinery gene
P3T65_RS07960 (P3T65_07960)	pilU	1713046..1714191 (+)	1146	WP_170858008.1	type IV pilus ATPase PilU	Machinery gene
P3T65_RS07965 (P3T65_07965)	-	1714266..1714670 (+)	405	WP_024766756.1	NINE protein	-
P3T65_RS07970 (P3T65_07970)	-	1714895..1716268 (+)	1374	WP_277369189.1	cystathionine beta-synthase	-
P3T65_RS07975 (P3T65_07975)	-	1716265..1717434 (+)	1170	WP_277369190.1	trans-sulfuration enzyme family protein	-
P3T65_RS07980 (P3T65_07980)	-	1717524..1717895 (-)	372	WP_277369191.1	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42596.96 Da
• Isoelectric Point: 6.7255

>NTDB_id=805261 P3T65_RS07960 WP_170858008.1 1713046..1714191(+) (pilU) [Pseudomonas nitroreducens strain L4]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYIQGEITYEDALAYADSANDLRLMIKLGSETDADHLTTMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=805261 P3T65_RS07960 WP_170858008.1 1713046..1714191(+) (pilU) [Pseudomonas nitroreducens strain L4]
ATGGAATTCGAGAAGCTGTTGCGCCTGATGGTGGAAAAGGGAGCCTCCGACCTGTTCATCACAGCGGGCGTACCACCGTC
GATGAAGGTCAACGGCAAGGTGTTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAAACCGTGCTGTCGG
TGATGAACGAGCAGCAGCGTCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCGTCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
TGACCTGAAACTGCCGGAAATCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACCTCGCTGGCGGCCATGATCGGCTACCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAT
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAAGTGGGCCTGGATACCGATACCTTCGAAGT
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCATTTCTTCCCCGCAGACCGTCACGGCCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTACCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAAGTCCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACATCCAGGGCGAGATCACCTACGAAGATGCGCTGGCCTATGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGTTCGGAAACCGACGCCGACCACTTGACCACCATGACCCAGGGCCTGACCCTGGAGATGAGCGACG
ACGATCCCAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.451	100	0.885
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	60.286	91.864	0.554
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383