Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVE35_RS22580 Genome accession   NZ_CP120359
Coordinates   4926263..4927297 (-) Length   344 a.a.
NCBI ID   WP_277373045.1    Uniprot ID   -
Organism   Pseudomonas sp. AA-38     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4921263..4932297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE35_RS22555 - 4921509..4922159 (-) 651 WP_268179311.1 DUF2076 domain-containing protein -
  PVE35_RS22560 - 4922268..4922666 (-) 399 WP_268179312.1 TM2 domain-containing protein -
  PVE35_RS22565 - 4922814..4924025 (+) 1212 WP_268179313.1 putative DNA modification/repair radical SAM protein -
  PVE35_RS22570 - 4924025..4924849 (+) 825 WP_277373043.1 TIGR03915 family putative DNA repair protein -
  PVE35_RS22575 pilU 4924991..4926136 (-) 1146 WP_277373044.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVE35_RS22580 pilT 4926263..4927297 (-) 1035 WP_277373045.1 type IV pilus twitching motility protein PilT Machinery gene
  PVE35_RS22585 - 4927363..4928055 (+) 693 WP_277373046.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVE35_RS22590 proC 4928073..4928888 (+) 816 WP_277373047.1 pyrroline-5-carboxylate reductase -
  PVE35_RS22595 - 4928944..4929537 (+) 594 WP_277373048.1 YggT family protein -
  PVE35_RS22600 - 4929540..4929830 (+) 291 WP_277373049.1 DUF167 domain-containing protein -
  PVE35_RS22605 - 4930041..4932008 (+) 1968 WP_277373050.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37919.60 Da        Isoelectric Point: 6.8887

>NTDB_id=805204 PVE35_RS22580 WP_277373045.1 4926263..4927297(-) (pilT) [Pseudomonas sp. AA-38]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVAKGLVSRDSAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=805204 PVE35_RS22580 WP_277373045.1 4926263..4927297(-) (pilT) [Pseudomonas sp. AA-38]
ATGGATATTACCGAGCTGCTTGCCTTCAGCGCCAAACAAGGCGCGTCGGATTTGCACCTCTCGGCCGGTTTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGCCGCATCAACCTGCCACCGCTGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGTGTCGCGCGC
TTCCGGGTCAACGCCTTCAATCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCAAGGTGCTGACCATGGA
CGACCTGGGCATGGGTGAGGTGTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCAACGGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTCAACAGCAACAAGTACCACCATATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGATACCCATGGCTTCTCCGA
AGCGCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCCGCCGAGGAGAAATCCATGGTGCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGTGGTGGTCGCGTGGCGGCCCACGAAATCATGATCGGTACGCCGGCCATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGCGCGTTGGGTATGCAGACCCTCGATGCCTGCCTC
AAGACCCTGGTGGCCAAGGGCCTGGTCAGCCGCGACAGCGCGCGGGAAAAGGCCAAGAGCCCGGAAAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398