Detailed information NTG0805203

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PVE35_RS22575
• Genome accession: NZ_CP120359
• Coordinates: 4924991..4926136 (-)
• Length: 381 a.a.
• NCBI ID: WP_277373044.1
• Uniprot ID: -
• Organism: Pseudomonas sp. AA-38
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4919991..4931136

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PVE35_RS22550	ntrC	4920025..4921458 (+)	1434	WP_268179310.1	nitrogen regulation protein NR(I)	-
PVE35_RS22555	-	4921509..4922159 (-)	651	WP_268179311.1	DUF2076 domain-containing protein	-
PVE35_RS22560	-	4922268..4922666 (-)	399	WP_268179312.1	TM2 domain-containing protein	-
PVE35_RS22565	-	4922814..4924025 (+)	1212	WP_268179313.1	putative DNA modification/repair radical SAM protein	-
PVE35_RS22570	-	4924025..4924849 (+)	825	WP_277373043.1	TIGR03915 family putative DNA repair protein	-
PVE35_RS22575	pilU	4924991..4926136 (-)	1146	WP_277373044.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PVE35_RS22580	pilT	4926263..4927297 (-)	1035	WP_277373045.1	type IV pilus twitching motility protein PilT	Machinery gene
PVE35_RS22585	-	4927363..4928055 (+)	693	WP_277373046.1	YggS family pyridoxal phosphate-dependent enzyme	-
PVE35_RS22590	proC	4928073..4928888 (+)	816	WP_277373047.1	pyrroline-5-carboxylate reductase	-
PVE35_RS22595	-	4928944..4929537 (+)	594	WP_277373048.1	YggT family protein	-
PVE35_RS22600	-	4929540..4929830 (+)	291	WP_277373049.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42624.93 Da
• Isoelectric Point: 6.7257

>NTDB_id=805203 PVE35_RS22575 WP_277373044.1 4924991..4926136(-) (pilU) [Pseudomonas sp. AA-38]
MEFEKLLRLMVEKGGSDLFITTGVPPSMKVNGKIMPVTKNPMTPEQARETVLGVMNDAQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDELKLPEILKKLSLTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTDSFEVALKNTLRQAPDVIMIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEIHELKSLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLSLEISEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=805203 PVE35_RS22575 WP_277373044.1 4924991..4926136(-) (pilU) [Pseudomonas sp. AA-38]
ATGGAATTCGAGAAGCTGCTGCGTCTGATGGTGGAAAAGGGTGGTTCGGATCTGTTCATCACCACCGGTGTGCCGCCTTC
GATGAAGGTCAATGGCAAGATCATGCCCGTGACCAAGAACCCCATGACCCCGGAGCAGGCTCGCGAAACCGTGCTGGGGG
TGATGAACGATGCCCAGCGCCGCGATTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCGCGCGGCATCGGCCGC
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAAACCAACATTCCCACCCTCGA
TGAGCTGAAGCTGCCGGAAATCCTCAAGAAACTGTCGCTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CGGGTAAATCCACCTCGCTGGCGGCGATGATCGGCTACCGTAACCGCAACAGCAGTGGCCACATCATTTCCATCGAGGAT
CCGATCGAGTACATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGTGAGGTGGGCATCGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCATGATCGGCGAGGTGCGTACCCGCGAAACCATGGATCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGCCGGATCGACACAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGTCGCGCGGTGATCGAGGTGCTGATCAACACGCCGCTGGCCGCCGACCTGA
TTCGCAAGGGCGAGATTCACGAACTCAAGAGCCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAGGACGCGCTGCTTTATGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGATGGCGACCACCTGACGAGCATGGCGCAGGGCCTGTCGCTGGAGATCAGCGAGG
AAGACCCAGGGCGTCGTTTTCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	89.239	100	0.892
pilU	Acinetobacter baylyi ADP1	63.585	93.701	0.596
pilU	Vibrio cholerae strain A1552	58	91.864	0.533
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.964	88.189	0.37