Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CupriaWKF_RS13880 Genome accession   NZ_CP119572
Coordinates   2992893..2993936 (-) Length   347 a.a.
NCBI ID   WP_276098425.1    Uniprot ID   -
Organism   Cupriavidus sp. WKF15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2987893..2998936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CupriaWKF_RS13850 (CupriaWKF_13850) - 2988876..2989262 (+) 387 WP_276098420.1 CidA/LrgA family protein -
  CupriaWKF_RS13855 (CupriaWKF_13855) - 2989265..2989990 (+) 726 WP_211945709.1 LrgB family protein -
  CupriaWKF_RS13860 (CupriaWKF_13860) - 2990076..2990447 (+) 372 WP_276098421.1 VOC family protein -
  CupriaWKF_RS13865 (CupriaWKF_13865) - 2990460..2991149 (+) 690 WP_276098422.1 YafY family protein -
  CupriaWKF_RS13870 (CupriaWKF_13870) - 2991153..2991647 (-) 495 WP_276098423.1 glutathione peroxidase -
  CupriaWKF_RS13875 (CupriaWKF_13875) pilU 2991692..2992831 (-) 1140 WP_276098424.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CupriaWKF_RS13880 (CupriaWKF_13880) pilT 2992893..2993936 (-) 1044 WP_276098425.1 type IV pilus twitching motility protein PilT Machinery gene
  CupriaWKF_RS13885 (CupriaWKF_13885) - 2994012..2994701 (+) 690 WP_276098426.1 YggS family pyridoxal phosphate-dependent enzyme -
  CupriaWKF_RS13890 (CupriaWKF_13890) proC 2994748..2995584 (+) 837 WP_276098427.1 pyrroline-5-carboxylate reductase -
  CupriaWKF_RS13895 (CupriaWKF_13895) ubiA 2995676..2996533 (-) 858 WP_276098428.1 4-hydroxybenzoate octaprenyltransferase -
  CupriaWKF_RS13900 (CupriaWKF_13900) - 2996686..2997171 (-) 486 WP_276098429.1 Dps family protein -
  CupriaWKF_RS13905 (CupriaWKF_13905) - 2997417..2998367 (-) 951 WP_276098430.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38448.24 Da        Isoelectric Point: 7.0152

>NTDB_id=799702 CupriaWKF_RS13880 WP_276098425.1 2992893..2993936(-) (pilT) [Cupriavidus sp. WKF15]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAHMAHKDVHAMVYDIMSDSQRKSYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSMISYSDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=799702 CupriaWKF_RS13880 WP_276098425.1 2992893..2993936(-) (pilT) [Cupriavidus sp. WKF15]
ATGGACATCGCGCAGCTATTGGCTTTCGCAGTCAAGAACAAGGCGTCCGATCTACACCTCTCGGCGGACATGCCGCCGAT
GGTGCGTATTCACGGCGACATGCGGCGCATCAACGTCGCCCACATGGCGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACTCCCAGCGCAAGAGCTACGAAGAGCGGCTGGAAATCGATTTCTCGTTTGAAATCGCCGGCCTGTCGCGT
TTCCGGGTCAACGCCTACAACACGCAGCGCGGCGCGGCCGCGGTATTCCGTACGATTCCCTCCAAGGTTCTCACGCTCGA
AGAACTGCGCGCGCCGGCGGTATTCGCCGACCTGTGCATGAAGCCACGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCAATGGTGGACCACCGCAACGAAAACGACATGGGTCACATCCTTACGGTCGAGGAC
CCGATCGAATTCGTCCACAACTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGTCCGCACACGCACTCGTTCGCCAA
TGCGTTGCGCTCCGCGCTGCGTGAAGACCCGGACGTGATCCTGGTGGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCGGAAACCGGCCACCTGGTTTTCGGCACGCTGCACACCAGCTCCGCGGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCGCCCGAAGAGAAGGACATGGTGCGCACCATGCTGTCCGAATCGCTGGAGGCCGTGATCTCGCAGAC
GCTGCTGAAGACGCGTGACGGCAACGGCCGCACCGCCGCGCACGAGATCATGATCGCCACGCCCGCGATTCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACCCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCATGATCAGCTACAGCGACGCACGGGCCATTGCCAAGAACCCCGACGCCTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.676

99.712

0.715
  pilT Acinetobacter baumannii D1279779

71.676

99.712

0.715
  pilT Acinetobacter baumannii strain A118

71.676

99.712

0.715
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Pseudomonas aeruginosa PAK

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

67.442

99.135

0.669
  pilT Neisseria gonorrhoeae MS11

67.442

99.135

0.669
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.118

0.634
  pilT Vibrio cholerae strain A1552

65.282

97.118

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392