Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CupriaWKF_RS13875 Genome accession   NZ_CP119572
Coordinates   2991692..2992831 (-) Length   379 a.a.
NCBI ID   WP_276098424.1    Uniprot ID   -
Organism   Cupriavidus sp. WKF15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2986692..2997831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CupriaWKF_RS13845 (CupriaWKF_13845) glcF 2987544..2988797 (+) 1254 WP_276098419.1 glycolate oxidase subunit GlcF -
  CupriaWKF_RS13850 (CupriaWKF_13850) - 2988876..2989262 (+) 387 WP_276098420.1 CidA/LrgA family protein -
  CupriaWKF_RS13855 (CupriaWKF_13855) - 2989265..2989990 (+) 726 WP_211945709.1 LrgB family protein -
  CupriaWKF_RS13860 (CupriaWKF_13860) - 2990076..2990447 (+) 372 WP_276098421.1 VOC family protein -
  CupriaWKF_RS13865 (CupriaWKF_13865) - 2990460..2991149 (+) 690 WP_276098422.1 YafY family protein -
  CupriaWKF_RS13870 (CupriaWKF_13870) - 2991153..2991647 (-) 495 WP_276098423.1 glutathione peroxidase -
  CupriaWKF_RS13875 (CupriaWKF_13875) pilU 2991692..2992831 (-) 1140 WP_276098424.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CupriaWKF_RS13880 (CupriaWKF_13880) pilT 2992893..2993936 (-) 1044 WP_276098425.1 type IV pilus twitching motility protein PilT Machinery gene
  CupriaWKF_RS13885 (CupriaWKF_13885) - 2994012..2994701 (+) 690 WP_276098426.1 YggS family pyridoxal phosphate-dependent enzyme -
  CupriaWKF_RS13890 (CupriaWKF_13890) proC 2994748..2995584 (+) 837 WP_276098427.1 pyrroline-5-carboxylate reductase -
  CupriaWKF_RS13895 (CupriaWKF_13895) ubiA 2995676..2996533 (-) 858 WP_276098428.1 4-hydroxybenzoate octaprenyltransferase -
  CupriaWKF_RS13900 (CupriaWKF_13900) - 2996686..2997171 (-) 486 WP_276098429.1 Dps family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42091.34 Da        Isoelectric Point: 6.6385

>NTDB_id=799701 CupriaWKF_RS13875 WP_276098424.1 2991692..2992831(-) (pilU) [Cupriavidus sp. WKF15]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKVTPVSQQPLNPTQALGLVRSIMNERQVKEFDDSRECNFA
ISVPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPPTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNANSY
GHIITIEDPIEYVHAHQNCVVTQREVGIDTESWHIALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLHSAASKDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=799701 CupriaWKF_RS13875 WP_276098424.1 2991692..2992831(-) (pilU) [Cupriavidus sp. WKF15]
ATGCTCGACCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTCGAGCTCATGGTGAGCAACCGCGGGTCGGACCTGTT
CATCACCTCGGACTTCCCGCCCGCCATCAAGGTCGACGGCAAGGTCACGCCGGTCTCGCAGCAGCCGCTCAATCCGACGC
AGGCGCTGGGCCTGGTGCGCTCGATCATGAACGAGCGCCAGGTCAAGGAGTTCGACGACAGCCGCGAGTGCAACTTCGCC
ATCTCGGTGCCCAAGGCCGGGCGCTTTCGCGTCTCGGCATTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGGACCAT
CAACACGCGCATCCCGTCGGTGGCGGACCTGGACCTGCCGCCGACGCTGCATGAGATTGTCATGTCCAAGCGCGGCCTGG
TGATCGTGACCGGCGCCACGGGCTCGGGCAAGTCGACCACGCTCGCGGCCATGCTGGACCACCGCAACGCGAATTCCTAC
GGCCACATCATCACCATCGAGGACCCGATCGAATACGTGCATGCGCACCAGAACTGCGTGGTCACGCAGCGCGAGGTCGG
CATCGACACCGAGTCCTGGCATATCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACCATGGAATACGCGATGCAGTACGCGGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCCATCGACCGCATCGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCCATGGTCTCCCAGCGCCTGCTGCCGCGCGCGGGCCGCAAGGGGCGCGTGCCGGCCGTGGAAATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAACTCAAGGAAGTGATCAAGAAATCGCGCGAGCAG
GGCATGATTTCCTTCGACCAGGCATTGTTCGACCTGCACGAAAGCGGCAAGATCACGTATGAGGACGCGCTGCGCAATGC
CGATTCGCTCAATGACCTGCGGCTGATGATCAAGCTGCACAGCGCCGCCAGCAAGGATACCGACCTCGGTGCCGGTACCG
AGCACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.644

91.821

0.575
  pilU Acinetobacter baylyi ADP1

57.382

94.723

0.544
  pilU Vibrio cholerae strain A1552

52.893

95.778

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

88.918

0.398
  pilT Pseudomonas stutzeri DSM 10701

43.917

88.918

0.391
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter nosocomialis M2

43.544

87.863

0.383
  pilT Acinetobacter baumannii D1279779

43.544

87.863

0.383
  pilT Acinetobacter baumannii strain A118

43.544

87.863

0.383
  pilT Legionella pneumophila strain ERS1305867

45.031

84.96

0.383
  pilT Legionella pneumophila strain Lp02

45.031

84.96

0.383
  pilT Acinetobacter baylyi ADP1

43.243

87.863

0.38
  pilT Neisseria meningitidis 8013

40.882

89.71

0.367