Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   P0M04_RS03945 Genome accession   NZ_CP119520
Coordinates   886344..887387 (+) Length   347 a.a.
NCBI ID   WP_259448658.1    Uniprot ID   -
Organism   Telluria mixta strain ACM 1762     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 881344..892387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M04_RS03925 (P0M04_03935) - 883161..884162 (+) 1002 WP_259448662.1 family 43 glycosylhydrolase -
  P0M04_RS03930 (P0M04_03940) - 884249..884743 (-) 495 WP_259448661.1 hypothetical protein -
  P0M04_RS03935 (P0M04_03945) proC 884753..885571 (-) 819 WP_259448660.1 pyrroline-5-carboxylate reductase -
  P0M04_RS03940 (P0M04_03950) - 885579..886280 (-) 702 WP_259448659.1 YggS family pyridoxal phosphate-dependent enzyme -
  P0M04_RS03945 (P0M04_03955) pilT 886344..887387 (+) 1044 WP_259448658.1 type IV pilus twitching motility protein PilT Machinery gene
  P0M04_RS03950 (P0M04_03960) pilU 887397..888533 (+) 1137 WP_259448657.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P0M04_RS03955 (P0M04_03965) - 888782..891655 (+) 2874 WP_259448656.1 TonB-dependent receptor -
  P0M04_RS03960 (P0M04_03970) - 891766..892197 (-) 432 WP_259448655.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38033.70 Da        Isoelectric Point: 6.7267

>NTDB_id=799415 P0M04_RS03945 WP_259448658.1 886344..887387(+) (pilT) [Telluria mixta strain ACM 1762]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKQYEEMLECDFSFAIPGLAR
FRVNAYNQERGASAVMRTIPSKILSLEELNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLVRRNVISSAAARSAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=799415 P0M04_RS03945 WP_259448658.1 886344..887387(+) (pilT) [Telluria mixta strain ACM 1762]
ATGGACATCTCGGAACTCCTGGCGTTCTCCGTTAAGAACAAAGCCTCGGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTACGCCGCATCAACCTGCCGCCGCTGGAACACAAGGACGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGCAGTACGAAGAGATGCTGGAGTGCGACTTCTCGTTCGCGATCCCCGGCCTCGCACGC
TTCCGCGTCAACGCGTACAACCAGGAACGCGGCGCGTCCGCCGTGATGCGGACGATTCCGTCGAAGATCCTGTCGCTGGA
AGAACTGAACGCGCCGAAGATCTTCGGCGACCTCGCGCTTAAGCCGCGCGGCCTCGTGCTCGTGACGGGTCCGACGGGCT
CCGGCAAGTCGACGACCCTGGCGGCGATGGTCAACCACCTGAACGAGAACGAGTACGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGCTGTCGTTCAACAA
CGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGACGCGATCCTCGTCGGCGAGCTGCGCGACCTGGAGACGATCCGCCTCG
CGCTGTCCGCCGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCCGCGAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGAGGAAAAAGAGATGGTGCGCGCGATGCTGTCGGAATCGCTGCAGGCCGTGATCTCGCAAAC
GCTGCTGAAAACGAAGGACGGCTCCGGCCGCGTGGCCGCGCACGAGATCATGATCGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAAGCGAAGATCGCGCAGATGTATTCCGCGATCCAGACCGGCTCCAACGTCGGCATGCAGACCCTGGACCAGAAC
CTGACGGACCTCGTGCGCCGCAACGTGATCTCGTCCGCGGCCGCACGCTCGGCCGCCAAGATCCCCGAGAACTTTCCGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.926

97.695

0.732
  pilT Legionella pneumophila strain ERS1305867

74.926

97.695

0.732
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.118

0.666
  pilT Vibrio cholerae strain A1552

68.546

97.118

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Acinetobacter baylyi ADP1

42.197

99.712

0.421
  pilU Vibrio cholerae strain A1552

41.298

97.695

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401