Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P0M04_RS03950 Genome accession   NZ_CP119520
Coordinates   887397..888533 (+) Length   378 a.a.
NCBI ID   WP_259448657.1    Uniprot ID   -
Organism   Telluria mixta strain ACM 1762     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 882397..893533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M04_RS03925 (P0M04_03935) - 883161..884162 (+) 1002 WP_259448662.1 family 43 glycosylhydrolase -
  P0M04_RS03930 (P0M04_03940) - 884249..884743 (-) 495 WP_259448661.1 hypothetical protein -
  P0M04_RS03935 (P0M04_03945) proC 884753..885571 (-) 819 WP_259448660.1 pyrroline-5-carboxylate reductase -
  P0M04_RS03940 (P0M04_03950) - 885579..886280 (-) 702 WP_259448659.1 YggS family pyridoxal phosphate-dependent enzyme -
  P0M04_RS03945 (P0M04_03955) pilT 886344..887387 (+) 1044 WP_259448658.1 type IV pilus twitching motility protein PilT Machinery gene
  P0M04_RS03950 (P0M04_03960) pilU 887397..888533 (+) 1137 WP_259448657.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P0M04_RS03955 (P0M04_03965) - 888782..891655 (+) 2874 WP_259448656.1 TonB-dependent receptor -
  P0M04_RS03960 (P0M04_03970) - 891766..892197 (-) 432 WP_259448655.1 hypothetical protein -
  P0M04_RS03965 (P0M04_03975) - 892259..892741 (+) 483 WP_259448654.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41985.43 Da        Isoelectric Point: 6.3175

>NTDB_id=799416 P0M04_RS03950 WP_259448657.1 887397..888533(+) (pilU) [Telluria mixta strain ACM 1762]
MERDQASKFMFDLLRLMVSKGGSDLFITSGFPPAMKIDGRMTPVSSQALTASHTADLARAIMNDKQAAGFELSKEANFAI
SPGDLGRFRVSAFMQMGAVGMVLRVITTTIPKFEDLDLPEILKDVIMTKRGLVIMVGATGSGKSTTLATMVGYRNENSYG
HIITIEDPVEFVHPHKNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNMKGLISQRLIPKKEAKGRVVAMEIMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEADKITYEDALRNADSVNDLRLNIKLNSKNAKNRDFSAGTETLGLI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=799416 P0M04_RS03950 WP_259448657.1 887397..888533(+) (pilU) [Telluria mixta strain ACM 1762]
ATGGAACGCGACCAGGCCTCCAAATTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAGGGCGGTTCCGACCTGTTCAT
CACGTCCGGCTTCCCGCCCGCGATGAAGATCGATGGCCGCATGACGCCCGTGTCCAGCCAGGCCCTCACGGCCTCGCACA
CGGCCGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGGCTTCGAGCTGTCGAAGGAAGCCAACTTCGCCATC
AGCCCGGGCGACCTGGGACGCTTCCGCGTCTCCGCCTTCATGCAGATGGGCGCCGTGGGCATGGTGCTGCGCGTGATCAC
GACGACGATCCCGAAGTTCGAGGACCTCGACCTGCCGGAGATCCTGAAGGACGTGATCATGACCAAGCGCGGTCTCGTGA
TCATGGTCGGCGCCACCGGCTCCGGTAAATCGACGACGCTTGCGACGATGGTCGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACGATCGAGGACCCCGTCGAATTCGTCCACCCGCACAAGAACTGCGTGATCACGCAGCGCGAGGTCGGCGT
CGACACGGACAGCTTCGAGGCCGCGCTGAAGAACTCCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGAGAAATCCGCG
ACCGCGAGACGATGGAACACGCGATCGCGTTCGCCGAGACGGGCCACCTCTGCCTGGCGACCCTGCACGCGAACAGCTCG
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGCCAGCAGCTCCTGATGGACCTGTCGCTGAACAT
GAAGGGCCTGATCTCGCAGCGCCTGATTCCGAAGAAGGAAGCGAAGGGACGCGTCGTCGCGATGGAGATCATGCTGAATT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTCCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTGTACGAGGCCGACAAGATCACGTACGAGGATGCGCTGCGCAATGCGGA
CTCCGTGAACGACCTGCGCCTGAACATCAAGCTCAACAGCAAAAACGCCAAGAACCGCGACTTCTCCGCGGGCACCGAGA
CGCTCGGTCTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

55.899

94.18

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Acinetobacter nosocomialis M2

42.598

87.566

0.373
  pilT Pseudomonas aeruginosa PAK

41.791

88.624

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.81

83.333

0.365
  pilT Vibrio cholerae strain A1552

43.81

83.333

0.365
  pilT Acinetobacter baylyi ADP1

43.125

84.656

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.624

0.365
  pilT Acinetobacter baumannii strain A118

43.533

83.862

0.365
  pilT Acinetobacter baumannii D1279779

43.533

83.862

0.365