Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWR52_RS04135 Genome accession   NZ_CP119446
Coordinates   845404..846384 (+) Length   326 a.a.
NCBI ID   WP_001055629.1    Uniprot ID   A0A398TTF2
Organism   Shigella boydii strain CIP 56-18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 840404..851384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWR52_RS04105 (PWR52_04110) - 840786..841772 (-) 987 WP_078167021.1 TRAP transporter substrate-binding protein -
  PWR52_RS04110 (PWR52_04115) hemW 842081..843217 (-) 1137 WP_078167022.1 radical SAM family heme chaperone HemW -
  PWR52_RS04115 (PWR52_04120) - 843210..843803 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  PWR52_RS04120 (PWR52_04125) yggU 843811..844101 (-) 291 WP_078167023.1 DUF167 family protein YggU -
  PWR52_RS04125 (PWR52_04130) yggT 844098..844664 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  PWR52_RS04130 (PWR52_04135) yggS 844682..845386 (-) 705 WP_078167024.1 pyridoxal phosphate homeostasis protein -
  PWR52_RS04135 (PWR52_04140) pilT 845404..846384 (+) 981 WP_001055629.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWR52_RS04140 (PWR52_04145) ruvX 846560..846976 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  PWR52_RS04145 (PWR52_04150) - 846976..847539 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  PWR52_RS04150 (PWR52_04155) gshB 847648..848598 (-) 951 WP_000593264.1 glutathione synthase -
  PWR52_RS04155 (PWR52_04160) rsmE 848611..849342 (-) 732 WP_078167025.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWR52_RS04160 (PWR52_04165) endA 849422..850129 (-) 708 WP_000286500.1 deoxyribonuclease I -
  PWR52_RS04165 (PWR52_04170) - 850224..850721 (-) 498 WP_078167026.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35884.09 Da        Isoelectric Point: 5.9959

>NTDB_id=799370 PWR52_RS04135 WP_001055629.1 845404..846384(+) (pilT) [Shigella boydii strain CIP 56-18]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVGKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=799370 PWR52_RS04135 WP_001055629.1 845404..846384(+) (pilT) [Shigella boydii strain CIP 56-18]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGGTAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A398TTF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.152

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362