Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RDV50_RS05625 Genome accession   NZ_CP133462
Coordinates   1166261..1167307 (-) Length   348 a.a.
NCBI ID   WP_003682881.1    Uniprot ID   -
Organism   Neisseria subflava strain LMG 5313     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1161261..1172307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV50_RS05610 (RDV50_05610) - 1161300..1162478 (+) 1179 WP_004519232.1 phosphoglycerate kinase -
  RDV50_RS05615 (RDV50_05615) yccS 1162644..1164779 (-) 2136 WP_137041356.1 YccS family putative transporter -
  RDV50_RS05620 (RDV50_05620) pilU 1164945..1166174 (-) 1230 WP_003682885.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RDV50_RS05625 (RDV50_05625) pilT 1166261..1167307 (-) 1047 WP_003682881.1 type IV pilus twitching motility protein PilT Machinery gene
  RDV50_RS05630 (RDV50_05630) - 1167425..1168120 (+) 696 WP_137041355.1 YggS family pyridoxal phosphate-dependent enzyme -
  RDV50_RS05635 (RDV50_05635) proC 1168197..1168994 (+) 798 WP_137041354.1 pyrroline-5-carboxylate reductase -
  RDV50_RS05640 (RDV50_05640) - 1168997..1169554 (+) 558 WP_137041353.1 YggT family protein -
  RDV50_RS05645 (RDV50_05645) dksA 1169788..1170204 (+) 417 WP_070631586.1 RNA polymerase-binding protein DksA -
  RDV50_RS10565 - 1170440..1170841 (-) 402 Protein_1104 transposase -
  RDV50_RS10570 - 1170947..1171552 (-) 606 Protein_1105 transposase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38407.01 Da        Isoelectric Point: 7.0897

>NTDB_id=799306 RDV50_RS05625 WP_003682881.1 1166261..1167307(-) (pilT) [Neisseria subflava strain LMG 5313]
MQITDLLAFGVKNKASDLHLSSGISPMIRVHGDIRRINLPEMSSEEVGTMITSVMNDHQRKLYQQDFEVDFSFELPNVAR
FRVNAFMTERGPAAVFRTIPGTVLTLEELRAPRIFQKIAENPRGLVLVTGPTGSGKSTTLAAMVNYINETQPAHILTIED
PIEFVHQSKKALINQRELHQHTNSFTNALRSALREDPDVILIGEMRDPETISLALTAAETGHLVFGTLHTTGAAKTIDRI
VDVFPAGEKEIVRSMLSESLRAIISQTLLKTRDGNGRVAAHEILISTPAVRNLIRENKIAQINAALQTGQAHGMQTLDQA
LQTLVRQGTISPELARSKAQNIENLSIF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=799306 RDV50_RS05625 WP_003682881.1 1166261..1167307(-) (pilT) [Neisseria subflava strain LMG 5313]
ATGCAGATTACCGACTTACTCGCGTTCGGCGTGAAAAACAAAGCCTCCGACCTCCATTTGAGTTCGGGTATTTCCCCTAT
GATACGCGTACACGGCGACATCCGCCGCATCAACCTGCCCGAGATGAGCTCGGAAGAAGTCGGTACCATGATTACCTCGG
TCATGAACGACCACCAGCGCAAGCTCTATCAACAGGATTTTGAAGTCGATTTCTCATTTGAGCTGCCTAATGTTGCCCGT
TTCCGTGTCAACGCCTTTATGACCGAACGCGGCCCGGCCGCCGTATTCCGTACCATTCCGGGCACCGTCCTGACGCTGGA
AGAATTGCGTGCGCCGCGCATTTTCCAAAAAATTGCCGAAAATCCGCGCGGGTTGGTCTTGGTAACCGGCCCGACCGGTT
CGGGTAAATCCACCACGCTAGCGGCGATGGTCAACTACATCAACGAAACCCAACCGGCCCACATTCTGACCATCGAAGAC
CCGATTGAGTTCGTCCACCAAAGCAAAAAGGCGCTCATCAACCAGCGCGAGCTGCATCAGCACACCAACAGCTTTACCAA
CGCCCTGCGTTCCGCCCTGCGCGAAGACCCAGACGTGATTTTGATCGGTGAGATGCGCGACCCTGAAACCATCTCCCTCG
CCCTGACCGCCGCCGAAACCGGTCACTTGGTTTTCGGCACGCTGCACACGACCGGCGCCGCCAAAACCATCGACCGTATT
GTCGACGTATTCCCTGCAGGCGAAAAAGAAATCGTGCGCTCCATGTTGTCCGAATCCTTGCGCGCCATCATTTCCCAAAC
CCTGCTGAAAACCCGCGACGGCAACGGCCGTGTCGCCGCACACGAGATCCTGATTTCTACACCGGCCGTGCGCAACCTCA
TCCGCGAGAACAAAATCGCCCAAATCAACGCCGCCCTCCAAACCGGTCAGGCCCACGGTATGCAAACCCTCGATCAAGCC
CTGCAAACCCTTGTCCGCCAAGGCACCATCAGCCCCGAGCTGGCACGCAGCAAGGCGCAAAACATCGAAAACCTCTCTAT
TTTCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

86.167

99.713

0.859
  pilT Neisseria gonorrhoeae MS11

86.167

99.713

0.859
  pilT Acinetobacter nosocomialis M2

68.116

99.138

0.675
  pilT Acinetobacter baumannii D1279779

68.116

99.138

0.675
  pilT Acinetobacter baumannii strain A118

68.116

99.138

0.675
  pilT Pseudomonas stutzeri DSM 10701

67.826

99.138

0.672
  pilT Pseudomonas aeruginosa PAK

67.536

99.138

0.67
  pilT Acinetobacter baylyi ADP1

67.246

99.138

0.667
  pilT Legionella pneumophila strain Lp02

65.103

97.989

0.638
  pilT Legionella pneumophila strain ERS1305867

65.103

97.989

0.638
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.706

97.701

0.632
  pilT Vibrio cholerae strain A1552

64.706

97.701

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.662

95.115

0.491
  pilU Pseudomonas stutzeri DSM 10701

41.092

100

0.411
  pilU Vibrio cholerae strain A1552

39.655

100

0.397
  pilU Acinetobacter baylyi ADP1

39.08

100

0.391