Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PTW35_RS02320 Genome accession   NZ_CP119423
Coordinates   455095..456132 (+) Length   345 a.a.
NCBI ID   WP_281026384.1    Uniprot ID   -
Organism   Photobacterium sp. DA100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450095..461132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTW35_RS02285 (PTW35_02285) hemW 450107..451270 (-) 1164 WP_281026377.1 radical SAM family heme chaperone HemW -
  PTW35_RS02290 (PTW35_02290) - 451274..451867 (-) 594 WP_281026378.1 XTP/dITP diphosphatase -
  PTW35_RS02295 (PTW35_02295) - 451980..452417 (-) 438 WP_281026379.1 DUF4426 domain-containing protein -
  PTW35_RS02300 (PTW35_02300) yggU 452575..452868 (-) 294 WP_281026380.1 DUF167 family protein YggU -
  PTW35_RS02305 (PTW35_02305) - 452865..453419 (-) 555 WP_281026381.1 YggT family protein -
  PTW35_RS02310 (PTW35_02310) proC 453429..454247 (-) 819 WP_281026382.1 pyrroline-5-carboxylate reductase -
  PTW35_RS02315 (PTW35_02315) - 454361..455071 (-) 711 WP_281026383.1 YggS family pyridoxal phosphate-dependent enzyme -
  PTW35_RS02320 (PTW35_02320) pilT 455095..456132 (+) 1038 WP_281026384.1 type IV pilus twitching motility protein PilT Machinery gene
  PTW35_RS02325 (PTW35_02325) pilU 456148..457254 (+) 1107 WP_281026385.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PTW35_RS02330 (PTW35_02330) ruvX 457594..458022 (-) 429 WP_281026386.1 Holliday junction resolvase RuvX -
  PTW35_RS02335 (PTW35_02335) - 458019..458582 (-) 564 WP_281026387.1 YqgE/AlgH family protein -
  PTW35_RS02340 (PTW35_02340) gshB 458729..459676 (-) 948 WP_039459699.1 glutathione synthase -
  PTW35_RS02345 (PTW35_02345) rsmE 459710..460441 (-) 732 WP_281026388.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PTW35_RS02350 (PTW35_02350) - 460562..461068 (-) 507 WP_281026389.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38073.55 Da        Isoelectric Point: 6.2319

>NTDB_id=799183 PTW35_RS02320 WP_281026384.1 455095..456132(+) (pilT) [Photobacterium sp. DA100]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHGEVHRLVFDIMNDAQRREFEERLEVDFSFELPDVGR
FRVNAFKQARGSAAVFRTIPVSIPTLESLGVPDVFYQIAQCQRGLVLVTGATGSGKSTTIAALVDYINENFNRHILTIED
PVEFVHTGKRCLINQREVHRDTHSFHNALRSSLREDPDVIVVGELRDRETISLALTAAETGHLVLGTLHTSSAAKTVDRV
IDVFPGNDKAMVRSMLSESLRAVVSQNLLKCTSGGRIAAHEVMMATPAIRNLIREDKVAQMFSMIQTGSAMGMQTMEQSV
KMLVAQGLVDAEEGRRVVDSGLSQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=799183 PTW35_RS02320 WP_281026384.1 455095..456132(+) (pilT) [Photobacterium sp. DA100]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAACGCCTCTGATCTGCACCTTTCTGCGGGGGTTCCGCCAAT
GATCCGTGTTGATGGTGATGTGCGCAAGCTGAGCCTGCCGGCCCTCGAGCACGGTGAAGTACACCGTCTGGTATTCGATA
TAATGAATGACGCCCAGCGGCGGGAGTTCGAAGAGCGGCTGGAAGTCGACTTTTCGTTCGAGTTGCCCGATGTCGGCCGC
TTCCGTGTTAATGCCTTCAAGCAGGCACGCGGCAGCGCGGCGGTATTCAGAACCATCCCGGTTAGCATTCCAACCCTGGA
GTCGCTGGGAGTGCCTGATGTGTTCTATCAGATCGCCCAGTGCCAACGGGGCCTGGTGCTGGTGACAGGGGCAACGGGAT
CGGGTAAGTCCACCACCATCGCCGCGCTGGTGGATTACATCAACGAGAATTTCAACCGCCATATCCTGACCATTGAGGAT
CCGGTGGAGTTTGTCCATACCGGCAAGCGCTGCCTGATCAACCAGCGGGAGGTCCACCGCGATACCCACAGTTTCCATAA
TGCCCTGCGCTCTTCGCTACGTGAAGATCCGGATGTGATTGTGGTCGGTGAGCTGCGCGATCGTGAAACGATCAGCTTGG
CACTGACGGCAGCAGAAACCGGGCATTTGGTGCTGGGTACCTTGCATACCAGCTCGGCGGCTAAGACCGTTGATAGGGTT
ATCGATGTGTTCCCGGGCAACGACAAGGCGATGGTGCGCTCGATGTTGTCGGAGTCGCTCCGCGCCGTGGTATCGCAGAA
CCTGCTCAAGTGCACCAGTGGCGGCCGTATTGCCGCCCACGAGGTGATGATGGCAACCCCGGCGATCCGCAACCTGATCC
GCGAAGATAAGGTGGCCCAGATGTTCTCGATGATCCAGACCGGCTCGGCGATGGGGATGCAGACTATGGAGCAGAGCGTC
AAGATGTTGGTAGCCCAAGGGCTGGTTGATGCCGAGGAAGGCCGCCGGGTAGTTGATTCTGGCTTGTCGCAATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.783

100

0.748
  pilT Vibrio cholerae strain A1552

74.783

100

0.748
  pilT Acinetobacter baumannii D1279779

70.448

97.101

0.684
  pilT Acinetobacter baumannii strain A118

70.448

97.101

0.684
  pilT Acinetobacter nosocomialis M2

70.149

97.101

0.681
  pilT Pseudomonas aeruginosa PAK

69.254

97.101

0.672
  pilT Acinetobacter baylyi ADP1

67.353

98.551

0.664
  pilT Pseudomonas stutzeri DSM 10701

68.358

97.101

0.664
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.392

97.681

0.629
  pilT Neisseria gonorrhoeae MS11

64.095

97.681

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.387

98.841

0.478
  pilU Pseudomonas stutzeri DSM 10701

41.935

98.841

0.414
  pilU Vibrio cholerae strain A1552

41.52

99.13

0.412
  pilU Acinetobacter baylyi ADP1

39.13

93.333

0.365