Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PTW35_RS02325 Genome accession   NZ_CP119423
Coordinates   456148..457254 (+) Length   368 a.a.
NCBI ID   WP_281026385.1    Uniprot ID   -
Organism   Photobacterium sp. DA100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451148..462254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTW35_RS02290 (PTW35_02290) - 451274..451867 (-) 594 WP_281026378.1 XTP/dITP diphosphatase -
  PTW35_RS02295 (PTW35_02295) - 451980..452417 (-) 438 WP_281026379.1 DUF4426 domain-containing protein -
  PTW35_RS02300 (PTW35_02300) yggU 452575..452868 (-) 294 WP_281026380.1 DUF167 family protein YggU -
  PTW35_RS02305 (PTW35_02305) - 452865..453419 (-) 555 WP_281026381.1 YggT family protein -
  PTW35_RS02310 (PTW35_02310) proC 453429..454247 (-) 819 WP_281026382.1 pyrroline-5-carboxylate reductase -
  PTW35_RS02315 (PTW35_02315) - 454361..455071 (-) 711 WP_281026383.1 YggS family pyridoxal phosphate-dependent enzyme -
  PTW35_RS02320 (PTW35_02320) pilT 455095..456132 (+) 1038 WP_281026384.1 type IV pilus twitching motility protein PilT Machinery gene
  PTW35_RS02325 (PTW35_02325) pilU 456148..457254 (+) 1107 WP_281026385.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PTW35_RS02330 (PTW35_02330) ruvX 457594..458022 (-) 429 WP_281026386.1 Holliday junction resolvase RuvX -
  PTW35_RS02335 (PTW35_02335) - 458019..458582 (-) 564 WP_281026387.1 YqgE/AlgH family protein -
  PTW35_RS02340 (PTW35_02340) gshB 458729..459676 (-) 948 WP_039459699.1 glutathione synthase -
  PTW35_RS02345 (PTW35_02345) rsmE 459710..460441 (-) 732 WP_281026388.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PTW35_RS02350 (PTW35_02350) - 460562..461068 (-) 507 WP_281026389.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41087.19 Da        Isoelectric Point: 6.2712

>NTDB_id=799184 PTW35_RS02325 WP_281026385.1 456148..457254(+) (pilU) [Photobacterium sp. DA100]
MTLQHALNEMVSRKASDLYITVDSPCLLRIDGHLHPLGETLDLQAVERMVAEAMDTGLEAEYRASKEANFALVRGEYRFR
ISAFWQRELPGMVIRRIETEIPDIFSLNLPMDMQKMALAKRGLVLVVGATGSGKSTSMAAMTGYRNANRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTPETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERREQFLFDLSLNLKCILAQQLIPDAHGVGRHGAFELLINTPRVADLIRRGELHEIKEAMAKSNESGMMTFDQSLY
ELYSAGKITEQDALHHADSANDLRLMIKVGSKSQLSNNSLSGVTVDMG

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=799184 PTW35_RS02325 WP_281026385.1 456148..457254(+) (pilU) [Photobacterium sp. DA100]
ATGACTTTGCAACACGCTCTCAACGAAATGGTCAGCCGCAAGGCGTCGGATCTCTACATTACAGTCGATTCCCCTTGCTT
GCTGCGCATCGATGGCCACCTCCATCCGCTCGGCGAGACGCTTGATCTGCAGGCGGTCGAGCGGATGGTTGCCGAGGCGA
TGGACACCGGGCTGGAAGCGGAGTACCGCGCCAGCAAGGAAGCCAATTTTGCTCTGGTAAGGGGGGAATATCGTTTCCGG
ATCAGTGCGTTCTGGCAGCGTGAACTGCCGGGGATGGTGATCCGCCGCATCGAAACCGAGATCCCGGATATCTTCTCGCT
CAATCTGCCGATGGACATGCAGAAGATGGCGCTGGCCAAACGCGGGCTGGTGCTGGTGGTGGGGGCGACCGGTTCGGGTA
AGTCGACCTCAATGGCGGCGATGACCGGATACCGCAACGCCAACCGTAGCGGCCATATCCTGACTGTCGAAGACCCGATT
GAGTTTGTCCACAAGCACAACAAGTGTATCGTCACCCAGCGCGAAGTAGGGCTGGATACCGAAAGCTACGAAGTTGCGCT
GAAGAACTCCCTGCGCCAGGCGCCGGATATGATCCTGATCGGCGAGATCCGCACGCCCGAGACCATGGAGTATGCGATGA
ACTTCGCCGAGACCGGCCACCTGTGTATGGCGACCCTTCATGCCAACAATGCCAACCAGGCGCTGGAGCGGATCCTGCAT
CTGGTCCCGAAAGAGCGGCGCGAGCAGTTCCTGTTCGATCTGTCGCTGAACCTCAAGTGCATCCTGGCCCAGCAGTTGAT
CCCCGATGCCCACGGTGTCGGTAGGCATGGGGCCTTCGAGCTGTTGATCAACACCCCGCGGGTGGCCGATCTGATCCGCC
GTGGTGAGCTGCATGAAATCAAAGAGGCAATGGCCAAGTCGAACGAGTCGGGCATGATGACCTTCGACCAGTCGCTCTAT
GAGCTCTACAGCGCCGGCAAGATCACCGAGCAAGATGCCTTGCACCACGCCGACTCGGCCAATGATCTGCGCCTGATGAT
CAAAGTCGGCAGCAAGAGCCAGCTTAGCAACAATTCGCTGTCTGGAGTGACGGTCGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

69.21

99.728

0.69
  pilU Pseudomonas stutzeri DSM 10701

58.592

96.467

0.565
  pilU Acinetobacter baylyi ADP1

53.09

96.739

0.514