Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PY254_RS06305 Genome accession   NZ_CP119392
Coordinates   1482439..1483476 (+) Length   345 a.a.
NCBI ID   WP_281014625.1    Uniprot ID   -
Organism   Rhodanobacter sp. AS-Z3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1477439..1488476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY254_RS06285 (PY254_06285) - 1477665..1478468 (+) 804 WP_281014621.1 cobalamin-binding protein -
  PY254_RS06290 (PY254_06290) - 1478508..1480604 (-) 2097 WP_281014622.1 M13 family metallopeptidase -
  PY254_RS06295 (PY254_06295) - 1480681..1481256 (-) 576 WP_281014623.1 YggT family protein -
  PY254_RS06300 (PY254_06300) proC 1481253..1482077 (-) 825 WP_281014624.1 pyrroline-5-carboxylate reductase -
  PY254_RS06305 (PY254_06305) pilT 1482439..1483476 (+) 1038 WP_281014625.1 type IV pilus twitching motility protein PilT Machinery gene
  PY254_RS06310 (PY254_06310) pilU 1483645..1484814 (+) 1170 WP_281014626.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PY254_RS06315 (PY254_06315) - 1484898..1485350 (-) 453 WP_345781829.1 BON domain-containing protein -
  PY254_RS06320 (PY254_06320) - 1485521..1487302 (-) 1782 WP_281014627.1 hypothetical protein -
  PY254_RS06325 (PY254_06325) - 1487392..1488318 (-) 927 WP_281014628.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38579.22 Da        Isoelectric Point: 6.8317

>NTDB_id=798787 PY254_RS06305 WP_281014625.1 1482439..1483476(+) (pilT) [Rhodanobacter sp. AS-Z3]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPRIFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHVNKNEYGHFLTIED
PIEFVHTSQKCLVNQREIHRDTLGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVVSQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCL
QDLVKRGLVTRQQAYGYAKNKDNFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=798787 PY254_RS06305 WP_281014625.1 1482439..1483476(+) (pilT) [Rhodanobacter sp. AS-Z3]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTAAAGAACAAAGCCTCGGACTTGCATCTGTCCGCAGGTCTGCCGCCGAT
GATCCGTGTTGATGGCGATGTTCGCCGCATCAACATTCCCGCGCTTGAACACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAAGAGTTTTACGAAACCGACTTCTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTTCGTACCATTCCCTCCGAAGTGCTGACCCTGGA
AGACCTGGGTTGCCCGCGCATCTTCAAGGAATTGATCGAACAGCCGCAGGGCCTGATCCTGGTAACCGGTCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGATCACGTCAACAAGAACGAATACGGTCACTTCCTCACCATCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGGTCAATCAGCGCGAAATTCACCGTGACACGCTGGGCTTCAACGA
GGCCCTGCGTTCGGCGCTGCGCGAGGACCCGGACTACATTCTGGTGGGCGAGTTGCGCGATCTGGAAACCATCCGGCTGG
CGCTGACGGCCGCGGAAACCGGCCACTTGGTGTTCGCTACCGTGCATACCAGCTCGGCGGCGAAGACCATCGATCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTGCGTTCGATGCTTTCGGAGTCGCTGCGTGCGGTGGTTTCACAGTC
GTTGCTGAAAAAGGTTGGCGGAGGCCGCATTGCGGCGCACGAAATCATGGTGGGCATTCCCGCCATCCGCAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACCGGTCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTG
CAGGACCTGGTCAAGCGGGGCCTGGTGACGCGCCAGCAGGCTTACGGTTATGCGAAGAACAAGGACAACTTCAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Acinetobacter nosocomialis M2

77.616

99.71

0.774
  pilT Acinetobacter baumannii D1279779

77.616

99.71

0.774
  pilT Acinetobacter baumannii strain A118

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

76.163

99.71

0.759
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.67

96.522

0.672
  pilT Vibrio cholerae strain A1552

69.67

96.522

0.672
  pilT Neisseria meningitidis 8013

63.478

100

0.635
  pilT Neisseria gonorrhoeae MS11

63.188

100

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.826

100

0.478
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Acinetobacter baylyi ADP1

38.617

100

0.388
  pilU Vibrio cholerae strain A1552

38.439

100

0.386