Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PX653_RS16180 Genome accession   NZ_CP119083
Coordinates   3669189..3670232 (+) Length   347 a.a.
NCBI ID   WP_277413797.1    Uniprot ID   -
Organism   Pseudoduganella chitinolytica strain ACM 3522     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3664189..3675232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX653_RS16145 (PX653_16145) - 3664196..3664687 (-) 492 WP_277413790.1 adenylyltransferase/cytidyltransferase family protein -
  PX653_RS16150 (PX653_16150) - 3664772..3665713 (+) 942 WP_277413791.1 hydrogen peroxide-inducible genes activator -
  PX653_RS16155 (PX653_16155) ubiA 3665774..3666649 (+) 876 WP_277413792.1 4-hydroxybenzoate octaprenyltransferase -
  PX653_RS16160 (PX653_16160) - 3666744..3667127 (+) 384 WP_277413793.1 YqjD family protein -
  PX653_RS16165 (PX653_16165) - 3667177..3667554 (-) 378 WP_277413794.1 VOC family protein -
  PX653_RS16170 (PX653_16170) proC 3667551..3668375 (-) 825 WP_277413795.1 pyrroline-5-carboxylate reductase -
  PX653_RS16175 (PX653_16175) - 3668378..3669085 (-) 708 WP_277413796.1 YggS family pyridoxal phosphate-dependent enzyme -
  PX653_RS16180 (PX653_16180) pilT 3669189..3670232 (+) 1044 WP_277413797.1 type IV pilus twitching motility protein PilT Machinery gene
  PX653_RS16185 (PX653_16185) pilU 3670242..3671378 (+) 1137 WP_277413798.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PX653_RS16190 (PX653_16190) - 3671387..3671872 (+) 486 WP_277413799.1 glutathione peroxidase -
  PX653_RS16195 (PX653_16195) - 3672037..3674487 (+) 2451 WP_277413800.1 bifunctional diguanylate cyclase/phosphodiesterase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37952.64 Da        Isoelectric Point: 6.7832

>NTDB_id=797590 PX653_RS16180 WP_277413797.1 3669189..3670232(+) (pilT) [Pseudoduganella chitinolytica strain ACM 3522]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKAYEEMLEIDFSFAIPGLAR
FRVNAYNQDRGASAVLRTIPSKILTLEELNAPKIFGDFALKPRGLVLVTGPTGSGKSTTLAAMVNHLNEHEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFSNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGAGRVAAHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLVRRNVISSAAARAAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=797590 PX653_RS16180 WP_277413797.1 3669189..3670232(+) (pilT) [Pseudoduganella chitinolytica strain ACM 3522]
ATGGACATCTCCGAACTTCTCGCATTCTCCGTCAAGAACAAGGCCTCGGACCTGCACTTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGCACGGCGATGTCCGCCGCATCAACCTGCCGCCGCTGGAGCACAAGGACGTGCACGGGATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCCTACGAGGAGATGCTGGAGATCGACTTCAGCTTCGCCATTCCCGGCCTGGCGCGC
TTTCGCGTCAATGCCTACAACCAGGACCGCGGCGCCTCCGCTGTGCTGCGCACGATTCCGTCGAAGATCCTGACCCTGGA
AGAACTGAACGCGCCGAAGATCTTCGGCGACTTTGCGCTGAAGCCGCGCGGCCTCGTCCTTGTCACCGGCCCGACCGGGT
CCGGCAAATCGACGACCCTGGCGGCGATGGTCAACCATCTCAACGAGCATGAGTATGGCCACATCCTGACGATCGAGGAC
CCCATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGCCCGCACACGCTGTCGTTCAGCAA
TGCGCTGCGCTCGGCGCTGCGGGAAGACCCGGATGCGATCCTGGTCGGCGAGCTGCGCGACCTGGAGACGATCCGCCTGG
CGCTGTCGGCTGCCGAAACGGGCCACCTGGTGTTCGGTACCCTGCACACGTCGTCCGCCGCGAAGACGATCGACCGGATC
GTCGACGTCTTCCCGGCCGAGGAGAAGGAGATGGTACGGGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTTGAAGACCAAGGACGGCGCCGGCCGCGTGGCGGCCCATGAGATCATGGTGGGCACGCCCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGATCGCCCAGATGTACTCGGCGATCCAGACCGGCAGCAATGTCGGCATGCAGACCCTGGACCAGAAC
CTGACGGACCTCGTGCGCCGCAACGTGATCTCGTCCGCCGCCGCCCGCGCCGCCGCCAAGATCCCCGAGAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Neisseria meningitidis 8013

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

95.677

0.669
  pilT Vibrio cholerae strain A1552

69.88

95.677

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Vibrio cholerae strain A1552

41.593

97.695

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.667

93.372

0.389