Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PX653_RS16185 Genome accession   NZ_CP119083
Coordinates   3670242..3671378 (+) Length   378 a.a.
NCBI ID   WP_277413798.1    Uniprot ID   -
Organism   Pseudoduganella chitinolytica strain ACM 3522     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3665242..3676378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX653_RS16155 (PX653_16155) ubiA 3665774..3666649 (+) 876 WP_277413792.1 4-hydroxybenzoate octaprenyltransferase -
  PX653_RS16160 (PX653_16160) - 3666744..3667127 (+) 384 WP_277413793.1 YqjD family protein -
  PX653_RS16165 (PX653_16165) - 3667177..3667554 (-) 378 WP_277413794.1 VOC family protein -
  PX653_RS16170 (PX653_16170) proC 3667551..3668375 (-) 825 WP_277413795.1 pyrroline-5-carboxylate reductase -
  PX653_RS16175 (PX653_16175) - 3668378..3669085 (-) 708 WP_277413796.1 YggS family pyridoxal phosphate-dependent enzyme -
  PX653_RS16180 (PX653_16180) pilT 3669189..3670232 (+) 1044 WP_277413797.1 type IV pilus twitching motility protein PilT Machinery gene
  PX653_RS16185 (PX653_16185) pilU 3670242..3671378 (+) 1137 WP_277413798.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PX653_RS16190 (PX653_16190) - 3671387..3671872 (+) 486 WP_277413799.1 glutathione peroxidase -
  PX653_RS16195 (PX653_16195) - 3672037..3674487 (+) 2451 WP_277413800.1 bifunctional diguanylate cyclase/phosphodiesterase -
  PX653_RS16200 (PX653_16200) glcF 3674506..3675726 (-) 1221 WP_277413801.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41658.17 Da        Isoelectric Point: 6.5942

>NTDB_id=797591 PX653_RS16185 WP_277413798.1 3670242..3671378(+) (pilU) [Pseudoduganella chitinolytica strain ACM 3522]
MERDQASKFMFDLLRLMVSKKGSDLFITAGFPPAMKIDGKLTPVSAQPLTAAHTADLARSIMNDKQTASFELTKEANFAI
SPGDLGRFRVSAFVQMSAVGMVLRTINSAIPKLDDLGLPEVLKDVIMAKRGLVVMVGATGSGKSTTLAAMVGYRNANSYG
HIITIEDPVEFVHPHGNCIVTQREVGVDTEDWEIALKNTLRQAPDVIQIGEIRDRQTMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPLKEAKGRAVAIEIMLNSPLISDLIFKGQVHEIKELMKKSRELG
MQTFDQSLFDLYEADKISYEDALRNADSVNDLRLAIKLEGKAAKTRDLSAGTEHLGLI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=797591 PX653_RS16185 WP_277413798.1 3670242..3671378(+) (pilU) [Pseudoduganella chitinolytica strain ACM 3522]
ATGGAACGCGACCAGGCATCCAAATTCATGTTCGACCTCTTGCGCCTGATGGTCAGCAAGAAGGGCTCGGACCTGTTCAT
CACGGCCGGCTTCCCGCCCGCGATGAAGATCGACGGCAAGCTTACGCCTGTCTCGGCCCAACCGCTCACCGCGGCGCACA
CGGCCGACCTGGCGCGCTCCATCATGAACGACAAGCAGACCGCGTCGTTCGAGCTGACCAAGGAAGCCAACTTCGCCATC
AGCCCGGGCGACCTGGGGCGCTTCCGCGTGTCGGCCTTCGTGCAGATGAGCGCCGTCGGCATGGTGCTGCGCACCATCAA
CAGCGCCATCCCCAAGCTGGACGACCTGGGCCTGCCGGAAGTGCTGAAGGACGTGATCATGGCCAAGCGCGGCCTCGTCG
TCATGGTCGGCGCCACCGGCTCGGGCAAGTCGACCACCCTGGCGGCGATGGTGGGCTACCGCAACGCCAACAGCTACGGC
CACATCATCACGATCGAGGACCCGGTGGAGTTCGTGCACCCGCACGGCAACTGCATCGTCACGCAGCGCGAGGTAGGCGT
CGACACGGAAGACTGGGAGATCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCCAGACGATGGACCATGCCATCGCCTTTGCCGAGACGGGCCACCTGTGCCTGGCCACCTTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAGGAGCGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GAAGGGCATGATCTCGCAGCGCCTGATCCCGCTGAAGGAAGCCAAGGGCCGTGCGGTCGCGATCGAGATCATGCTGAACT
CGCCGTTGATCTCGGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAGCTGATGAAGAAGTCGCGCGAACTGGGC
ATGCAAACCTTCGACCAGTCGCTGTTCGACCTGTACGAAGCCGACAAGATCAGCTACGAGGACGCGCTGCGCAACGCCGA
TTCCGTCAACGACCTGCGGTTGGCGATCAAGCTGGAAGGCAAGGCCGCCAAGACGCGCGACCTGTCCGCCGGTACCGAAC
ATCTGGGCCTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.451

91.534

0.59
  pilU Acinetobacter baylyi ADP1

59.384

94.444

0.561
  pilU Vibrio cholerae strain A1552

58.065

90.212

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

88.624

0.384
  pilT Pseudomonas aeruginosa PAK

42.985

88.624

0.381
  pilT Acinetobacter nosocomialis M2

41.994

87.566

0.368
  pilT Legionella pneumophila strain Lp02

41.994

87.566

0.368
  pilT Acinetobacter baumannii D1279779

41.994

87.566

0.368
  pilT Acinetobacter baumannii strain A118

41.994

87.566

0.368
  pilT Legionella pneumophila strain ERS1305867

41.994

87.566

0.368
  pilT Pseudomonas stutzeri DSM 10701

41.194

88.624

0.365
  pilT Acinetobacter baylyi ADP1

42.812

84.656

0.362