Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PYU98_RS20305 Genome accession   NZ_CP118988
Coordinates   4344882..4345916 (-) Length   344 a.a.
NCBI ID   WP_042063177.1    Uniprot ID   A0A6S5C6A2
Organism   Aeromonas allosaccharophila strain K520     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4339882..4350916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU98_RS20275 (PYU98_20275) glnK 4340461..4340799 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  PYU98_RS20280 (PYU98_20280) arfB 4341001..4341417 (+) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  PYU98_RS20285 (PYU98_20285) - 4341450..4341941 (+) 492 WP_139707985.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  PYU98_RS20290 (PYU98_20290) - 4342303..4342728 (-) 426 WP_275057004.1 hypothetical protein -
  PYU98_RS20295 (PYU98_20295) yaaA 4342923..4343696 (-) 774 WP_139707986.1 peroxide stress protein YaaA -
  PYU98_RS20300 (PYU98_20300) pilU 4343744..4344853 (-) 1110 WP_139708023.1 type IVa pilus ATPase TapU Machinery gene
  PYU98_RS20305 (PYU98_20305) pilT 4344882..4345916 (-) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  PYU98_RS20310 (PYU98_20310) - 4345956..4346657 (+) 702 WP_139707987.1 YggS family pyridoxal phosphate-dependent enzyme -
  PYU98_RS20315 (PYU98_20315) proC 4346800..4347624 (+) 825 WP_139707988.1 pyrroline-5-carboxylate reductase -
  PYU98_RS20320 (PYU98_20320) - 4347636..4348187 (+) 552 WP_042011910.1 YggT family protein -
  PYU98_RS20325 (PYU98_20325) yggU 4348187..4348489 (+) 303 WP_139707989.1 DUF167 family protein YggU -
  PYU98_RS20330 (PYU98_20330) - 4348533..4348952 (+) 420 WP_139707990.1 DUF4426 domain-containing protein -
  PYU98_RS20335 (PYU98_20335) - 4349053..4350249 (-) 1197 WP_042063182.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38291.00 Da        Isoelectric Point: 6.7439

>NTDB_id=797182 PYU98_RS20305 WP_042063177.1 4344882..4345916(-) (pilT) [Aeromonas allosaccharophila strain K520]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKISEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=797182 PYU98_RS20305 WP_042063177.1 4344882..4345916(-) (pilT) [Aeromonas allosaccharophila strain K520]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTATGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTTAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGTACCATCCCCAGCACGGTCTTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTTCGCAAGATCTCCGAATACCCGCGCGGTCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGTAAATCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATTCTCACCATTGAAGAC
CCCATCGAATTCGTTCATGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCAGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGTGACCTCGAGACCATTCGTCTGG
CCATGACTGCCGCCGAAACCGGCCATCTGGTGTTTGGTACCCTGCACACCTCGTCGGCAGCCAAAACCATCGACCGTATC
ATTGACGTCTTCCCCGGTGCGGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGTGGCGGCCGGGTGGCGGCACACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTGATCC
GTGAGGACAAGATCGCCCAGCTCTACTCTGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTGGTGGCATCCCTTGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5C6A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

99.709

0.776
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

77.551

99.709

0.773
  pilT Acinetobacter baumannii strain A118

77.551

99.709

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.802

99.709

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.353

98.837

0.715
  pilT Legionella pneumophila strain ERS1305867

72.353

98.837

0.715
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404