Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PYU98_RS20300 Genome accession   NZ_CP118988
Coordinates   4343744..4344853 (-) Length   369 a.a.
NCBI ID   WP_139708023.1    Uniprot ID   A0AAX3NZA3
Organism   Aeromonas allosaccharophila strain K520     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4338744..4349853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU98_RS20270 (PYU98_20270) - 4339195..4340202 (-) 1008 WP_139707984.1 Fe(3+) ABC transporter substrate-binding protein -
  PYU98_RS20275 (PYU98_20275) glnK 4340461..4340799 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  PYU98_RS20280 (PYU98_20280) arfB 4341001..4341417 (+) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  PYU98_RS20285 (PYU98_20285) - 4341450..4341941 (+) 492 WP_139707985.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  PYU98_RS20290 (PYU98_20290) - 4342303..4342728 (-) 426 WP_275057004.1 hypothetical protein -
  PYU98_RS20295 (PYU98_20295) yaaA 4342923..4343696 (-) 774 WP_139707986.1 peroxide stress protein YaaA -
  PYU98_RS20300 (PYU98_20300) pilU 4343744..4344853 (-) 1110 WP_139708023.1 type IVa pilus ATPase TapU Machinery gene
  PYU98_RS20305 (PYU98_20305) pilT 4344882..4345916 (-) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  PYU98_RS20310 (PYU98_20310) - 4345956..4346657 (+) 702 WP_139707987.1 YggS family pyridoxal phosphate-dependent enzyme -
  PYU98_RS20315 (PYU98_20315) proC 4346800..4347624 (+) 825 WP_139707988.1 pyrroline-5-carboxylate reductase -
  PYU98_RS20320 (PYU98_20320) - 4347636..4348187 (+) 552 WP_042011910.1 YggT family protein -
  PYU98_RS20325 (PYU98_20325) yggU 4348187..4348489 (+) 303 WP_139707989.1 DUF167 family protein YggU -
  PYU98_RS20330 (PYU98_20330) - 4348533..4348952 (+) 420 WP_139707990.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41078.97 Da        Isoelectric Point: 6.0896

>NTDB_id=797181 PYU98_RS20300 WP_139708023.1 4343744..4344853(-) (pilU) [Aeromonas allosaccharophila strain K520]
MNLDELLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSNDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVIRRIETRIPTFEDLQLPNILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLQIKLSGQETLGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=797181 PYU98_RS20300 WP_139708023.1 4343744..4344853(-) (pilU) [Aeromonas allosaccharophila strain K520]
ATGAATCTGGATGAATTGCTGAGCGAATTGGTCGAACGAAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
TCTCAAGGTCAATGGCCATCTGGTGTCGCTGGGGGGCGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAACGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCCATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGATACGTCGCATTGAGACCCGCATCCCCACTTTTGA
AGATCTGCAGTTGCCCAACATCTTGCAGGAGGTCGCCATGGCCAAGCGGGGGTTGGTGCTGTTTGTCGGGGCCACCGGGG
CGGGTAAGTCCACTACTCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGCCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGCCGTAGTCTGGTGACCCAGCGTGAAGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGCTGAAGAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACCGGCCACCTTTGCCTCGCCACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGGATC
CTCCATCTGGTGCCGCAAGACAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAACCTCAAGGCCATCGTTGCCCAGCA
ACTGGTGCCAAGCATGGATGGCAAGCGGCGTTTTGCCGCGTTCGAGGTGCTGCTCAATACCCCGCTCATCACTGACATCA
TCCGCAAGGGAGAGATGCACCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGCATGCAGACCTTCGATCAGGCG
CTATTCAGCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCTCACGCCGACTCGGCTAATGATTTGCGTCT
GCAAATCAAGCTCTCTGGTCAGGAGACGCTTGGCTCCGGCACGCTGGAGAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

60.615

97.019

0.588
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.233

91.87

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36