Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWO49_RS13520 Genome accession   NZ_CP118916
Coordinates   2997582..2998622 (-) Length   346 a.a.
NCBI ID   WP_210797611.1    Uniprot ID   -
Organism   Pseudoalteromonas ostreae strain hOe-124     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2992582..3003622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO49_RS13490 rsmE 2992733..2993467 (+) 735 WP_210797616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWO49_RS13495 gshB 2993522..2994475 (+) 954 WP_210797615.1 glutathione synthase -
  PWO49_RS13500 - 2994503..2995060 (+) 558 WP_210797614.1 YqgE/AlgH family protein -
  PWO49_RS13505 ruvX 2995095..2995541 (+) 447 WP_210797613.1 Holliday junction resolvase RuvX -
  PWO49_RS13510 - 2995504..2996430 (-) 927 WP_215909092.1 hypothetical protein -
  PWO49_RS13515 pilU 2996432..2997562 (-) 1131 WP_210797612.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWO49_RS13520 pilT 2997582..2998622 (-) 1041 WP_210797611.1 type IV pilus twitching motility protein PilT Machinery gene
  PWO49_RS13525 - 2998625..2999338 (+) 714 WP_275074492.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWO49_RS13530 proC 2999354..3000175 (+) 822 WP_210797609.1 pyrroline-5-carboxylate reductase -
  PWO49_RS13535 - 3000233..3000766 (+) 534 WP_210797608.1 YggT family protein -
  PWO49_RS13540 - 3000818..3001261 (+) 444 WP_210797607.1 DUF4426 domain-containing protein -
  PWO49_RS13545 - 3001265..3001864 (+) 600 WP_210797606.1 XTP/dITP diphosphatase -
  PWO49_RS13550 hemW 3001861..3002997 (+) 1137 WP_210797605.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38209.46 Da        Isoelectric Point: 6.4495

>NTDB_id=796316 PWO49_RS13520 WP_210797611.1 2997582..2998622(-) (pilT) [Pseudoalteromonas ostreae strain hOe-124]
MDITELLAFSVQHKASDLHLSSGVAPMIRVDGDVRRINIPALENKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDHINQHKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMIRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
TNLVNHGVVTNAEARAKAQDKNTFGS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=796316 PWO49_RS13520 WP_210797611.1 2997582..2998622(-) (pilT) [Pseudoalteromonas ostreae strain hOe-124]
ATGGATATTACCGAATTGTTGGCCTTTAGTGTGCAGCACAAAGCATCAGATTTACACTTATCATCAGGGGTTGCGCCAAT
GATCCGTGTTGATGGTGATGTGCGACGTATTAATATACCAGCGCTGGAAAACAAAGATGTTAACAGCCTAGTTTACGATA
TTATGAACGATAATCAACGTAAGGACTACGAACAAAACCTAGAAGTGGATTTTTCGTTTGAAGTGCCAAACTTAGCGCGT
TTTCGTGTTAATGCCTTTAACTCTAATCGAGGCCCAGCTGCTGTATTTCGTACCATTCCTAGTGAAGTGCTGACACTTGA
TGATTTAGGTGCGCCAGATATATTTAAAACAATCTCAGATACGCCGCGTGGTTTAGTGTTGGTAACAGGACCTACAGGCT
CTGGTAAATCGACCACATTGGCTGCGATGGTGGATCATATAAATCAACATAAGCATCATCATATTTTAACCATTGAAGAC
CCTATTGAGTTTGTGCACGACAATAAGCTATCGTTGATTAATCAACGTGAAGTGCATCGCGATACGCATAGTTTTTCTAA
TGCACTACGCAGTGCTTTACGTGAAGATCCAGACGTGATCTTGGTTGGTGAGTTGCGTGATTTAGAAACTATTCGCCTAG
CTATGACCGCAGCTGAAACCGGCCATTTAGTATTTGGTACTTTGCATACCACCTCTGCGCCAAAAACCATTGACCGTATT
ATCGATGTATTCCCTGGAGAAGAAAAAAGCATGATCCGCTCTATGCTTTCAGAATCATTGCGTGCGGTAATTTCACAAAC
CCTGTTGAAAAAAATTGGTGGTGGGCGGGTCGCCGCCCATGAAATTATGATTGCAGTGCCTGCGATCAGAAACTTGATCC
GCGAAGATAAAATTGCACAAATGTATTCGTCGATTCAAACTGGTGCATCGCACGGCATGCAAACCATGGATCAATGTTTA
ACCAATTTAGTTAACCACGGTGTAGTTACCAATGCCGAGGCGCGCGCTAAAGCACAAGATAAAAATACTTTCGGCAGTTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.453

99.422

0.76
  pilT Acinetobacter baumannii D1279779

76.453

99.422

0.76
  pilT Acinetobacter baumannii strain A118

76.453

99.422

0.76
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Pseudomonas stutzeri DSM 10701

75.581

99.422

0.751
  pilT Pseudomonas aeruginosa PAK

75.291

99.422

0.749
  pilT Legionella pneumophila strain Lp02

72.093

99.422

0.717
  pilT Legionella pneumophila strain ERS1305867

72.093

99.422

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria meningitidis 8013

68.986

99.711

0.688
  pilT Neisseria gonorrhoeae MS11

68.696

99.711

0.685
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.711

0.523
  pilU Vibrio cholerae strain A1552

40.816

99.133

0.405
  pilU Acinetobacter baylyi ADP1

39.595

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.776

99.133

0.384