Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HMEPL2_RS13950 Genome accession   NZ_AP022869
Coordinates   2944466..2945509 (+) Length   347 a.a.
NCBI ID   WP_044629498.1    Uniprot ID   A0A0D7UXJ5
Organism   Vreelandella aquamarina strain Eplume2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2939466..2950509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMEPL2_RS13920 (HMEPL2_27870) - 2939760..2940221 (-) 462 WP_044629492.1 DUF4426 domain-containing protein -
  HMEPL2_RS13925 (HMEPL2_27880) metW 2940258..2940854 (-) 597 WP_044629493.1 methionine biosynthesis protein MetW -
  HMEPL2_RS13930 (HMEPL2_27890) - 2940854..2942050 (-) 1197 WP_172515260.1 homoserine O-acetyltransferase -
  HMEPL2_RS13935 (HMEPL2_27900) - 2942124..2942717 (-) 594 WP_058578303.1 YggT family protein -
  HMEPL2_RS13940 (HMEPL2_27910) proC 2942818..2943645 (-) 828 WP_074211081.1 pyrroline-5-carboxylate reductase -
  HMEPL2_RS13945 (HMEPL2_27920) - 2943734..2944432 (-) 699 WP_074211080.1 YggS family pyridoxal phosphate-dependent enzyme -
  HMEPL2_RS13950 (HMEPL2_27930) pilT 2944466..2945509 (+) 1044 WP_044629498.1 type IV pilus twitching motility protein PilT Machinery gene
  HMEPL2_RS13955 (HMEPL2_27940) pilU 2945534..2946697 (+) 1164 WP_083602229.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HMEPL2_RS13960 (HMEPL2_27950) - 2946710..2947852 (-) 1143 WP_074211639.1 anhydro-N-acetylmuramic acid kinase -
  HMEPL2_RS13965 (HMEPL2_27960) tyrS 2947969..2949168 (+) 1200 WP_062363647.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38275.88 Da        Isoelectric Point: 6.5962

>NTDB_id=79596 HMEPL2_RS13950 WP_044629498.1 2944466..2945509(+) (pilT) [Vreelandella aquamarina strain Eplume2]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMENSDVRRLIYDIMNDRQRRDYEEHLEADFSFEVPGVAR
FRVNAFNQARGAGAVFRTIPNKVLSMETLGMGEVFQRLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINEQRFEHILTIED
PVEFVHTSKRCLINQREVHRDTHSFSGALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVISQTLLKRQGGGRVAAHEILIATSAVRNLVREDKVAQIYSAIQTGGALGMQTLNASL
AKLVKEGVVSMEEAQVRAKGTLTLKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=79596 HMEPL2_RS13950 WP_044629498.1 2944466..2945509(+) (pilT) [Vreelandella aquamarina strain Eplume2]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAGCAGAATGCATCCGACTTGCACCTTTCGGCGGGTCTGCCTCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTGCCCGCCATGGAGAACAGCGACGTTCGGCGGCTGATCTACGACA
TCATGAACGATCGGCAGCGCCGCGACTATGAAGAGCACTTAGAAGCGGACTTCTCGTTTGAAGTGCCGGGGGTGGCGCGC
TTTAGGGTCAATGCCTTTAACCAGGCACGCGGGGCGGGGGCGGTGTTCCGTACCATTCCCAATAAGGTGCTTTCCATGGA
AACCTTGGGCATGGGCGAGGTGTTCCAGCGCCTTTCGATGCTACCCAGGGGATTGGTGCTGGTGACCGGGCCCACGGGGT
CGGGCAAAAGCACGACCTTGGCGGCGATGATCGACTACATCAACGAACAGCGCTTCGAGCATATTCTGACCATCGAAGAT
CCGGTGGAGTTTGTGCACACCAGCAAGCGTTGCCTGATTAATCAGCGGGAGGTTCACCGCGATACCCATAGCTTCTCTGG
GGCGCTGCGAAGCGCGCTGCGCGAAGACCCGGACGTGATTTTGGTCGGTGAGTTGCGGGATCTCGAAACGATCCGTTTAG
CGCTCACCGCTGCTGAAACGGGGCACCTAGTATTTGGTACGCTGCACACGACTTCGGCGGCGAAAACCATCGACCGGATT
ATTGATGTATTCCCCGGCGAAGAGAAATCTATGGTGCGCTCGATGCTGTCTGAATCGCTCCAGGCGGTCATCTCGCAAAC
GCTGTTGAAGCGTCAGGGTGGCGGACGCGTGGCGGCCCACGAGATCCTGATTGCCACGTCTGCGGTGCGTAACCTCGTTC
GCGAAGATAAGGTGGCGCAGATCTACTCCGCCATCCAGACCGGCGGCGCACTGGGAATGCAGACCCTGAATGCCTCGCTC
GCTAAGCTGGTTAAAGAGGGCGTGGTGAGTATGGAGGAAGCCCAAGTAAGAGCTAAAGGCACGCTGACGTTAAAAGATGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D7UXJ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

79.646

97.695

0.778
  pilT Pseudomonas stutzeri DSM 10701

79.056

97.695

0.772
  pilT Acinetobacter nosocomialis M2

77.876

97.695

0.761
  pilT Acinetobacter baumannii D1279779

77.876

97.695

0.761
  pilT Acinetobacter baumannii strain A118

77.876

97.695

0.761
  pilT Acinetobacter baylyi ADP1

74.926

97.695

0.732
  pilT Legionella pneumophila strain Lp02

71.642

96.542

0.692
  pilT Legionella pneumophila strain ERS1305867

71.642

96.542

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

67.544

98.559

0.666
  pilT Neisseria gonorrhoeae MS11

67.251

98.559

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Pseudomonas stutzeri DSM 10701

40.173

99.712

0.401
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Acinetobacter baylyi ADP1

39.193

100

0.392

Multiple sequence alignment