Detailed information NTG0079597

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HMEPL2_RS13955
• Genome accession: NZ_AP022869
• Coordinates: 2945534..2946697 (+)
• Length: 387 a.a.
• NCBI ID: WP_083602229.1
• Uniprot ID: -
• Organism: Vreelandella aquamarina strain Eplume2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2940534..2951697

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMEPL2_RS13930 (HMEPL2_27890)	-	2940854..2942050 (-)	1197	WP_172515260.1	homoserine O-acetyltransferase	-
HMEPL2_RS13935 (HMEPL2_27900)	-	2942124..2942717 (-)	594	WP_058578303.1	YggT family protein	-
HMEPL2_RS13940 (HMEPL2_27910)	proC	2942818..2943645 (-)	828	WP_074211081.1	pyrroline-5-carboxylate reductase	-
HMEPL2_RS13945 (HMEPL2_27920)	-	2943734..2944432 (-)	699	WP_074211080.1	YggS family pyridoxal phosphate-dependent enzyme	-
HMEPL2_RS13950 (HMEPL2_27930)	pilT	2944466..2945509 (+)	1044	WP_044629498.1	type IV pilus twitching motility protein PilT	Machinery gene
HMEPL2_RS13955 (HMEPL2_27940)	pilU	2945534..2946697 (+)	1164	WP_083602229.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HMEPL2_RS13960 (HMEPL2_27950)	-	2946710..2947852 (-)	1143	WP_074211639.1	anhydro-N-acetylmuramic acid kinase	-
HMEPL2_RS13965 (HMEPL2_27960)	tyrS	2947969..2949168 (+)	1200	WP_062363647.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42135.21 Da
• Isoelectric Point: 6.8279

>NTDB_id=79597 HMEPL2_RS13955 WP_083602229.1 2945534..2946697(+) (pilU) [Vreelandella aquamarina strain Eplume2]
MSEPASGMTVTQWLHQLLGIMVEQQASDLLISVGAPPSLKTPKGLMPLGRQPLSSSQVRSLVLNAVPEKQRERFQEEHEA
NFALSLESAGRFRVSAFQQRNEPAMVVRRIAQTIPTLSALGVPSQLATLANAKRGLVLVVGGTGTGKSTTLAAMIQERNA
SVGGHIITIEDPIEYLHPHQRGIVNQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMEHALTFAETGHLCLATLH
ANNANQALERILHFFPHERHEQVLMDLSLNLRAVVAQQLLPTQQGGRCAAIEILLSSPRVVDLIRKGRIDELKQAMAGSR
DAGMQTFDQALYALYQAGQVSQQVALAHADSANDLRLMIKQGDAVASGKPLDAQVPPHLALRDGDDY

Nucleotide

• Length: 1164 bp

>NTDB_id=79597 HMEPL2_RS13955 WP_083602229.1 2945534..2946697(+) (pilU) [Vreelandella aquamarina strain Eplume2]
ATGAGTGAGCCAGCCAGCGGTATGACCGTGACCCAGTGGCTGCACCAACTGCTGGGTATTATGGTCGAGCAGCAGGCATC
AGACCTGCTGATTTCGGTGGGCGCACCGCCCAGTTTGAAAACGCCCAAGGGGCTAATGCCGCTGGGGCGTCAGCCGCTCT
CCTCTAGCCAGGTGCGTAGCCTGGTTCTGAATGCGGTTCCCGAAAAGCAGCGGGAGCGATTTCAAGAGGAGCACGAGGCG
AACTTTGCCCTAAGCCTGGAGAGTGCGGGGCGGTTTCGGGTAAGCGCCTTTCAACAGCGCAACGAGCCCGCCATGGTGGT
GCGCCGCATTGCGCAGACCATTCCGACCCTTAGCGCGCTGGGCGTGCCTAGCCAGTTGGCGACGTTGGCCAATGCCAAGC
GCGGGCTGGTGCTAGTGGTAGGCGGCACGGGAACGGGGAAATCCACTACGCTGGCGGCGATGATTCAGGAGCGTAACGCC
TCAGTCGGTGGGCATATCATCACCATTGAAGACCCCATCGAGTACCTGCATCCGCATCAGCGAGGCATCGTTAATCAGCG
CGAAGTGGGGGTGGATACGGAGTCCTTCGAGGTGGCGCTGAAAAATACGCTACGCCAAGCGCCGGATGTGATCTTGATTG
GTGAAGTGCGTACCCGCGAGACCATGGAGCACGCCCTCACTTTCGCGGAAACCGGGCATCTCTGTTTGGCGACATTGCAC
GCCAACAACGCCAACCAAGCCTTAGAGCGTATCCTTCACTTCTTTCCCCATGAGCGGCACGAGCAGGTTCTGATGGATCT
GTCGCTGAATCTGCGTGCCGTGGTCGCGCAGCAGCTGCTACCTACTCAGCAGGGCGGCCGCTGCGCGGCGATCGAGATTC
TGCTCTCATCGCCGCGGGTGGTGGATCTGATTCGTAAAGGGCGTATTGATGAATTGAAGCAGGCCATGGCAGGCTCCCGC
GACGCGGGGATGCAAACCTTTGATCAGGCGCTTTATGCGCTTTATCAAGCGGGCCAAGTCAGCCAGCAAGTGGCGCTGGC
CCATGCGGACTCCGCCAACGACCTGCGGCTGATGATCAAGCAGGGCGACGCCGTCGCCAGCGGTAAGCCGTTAGATGCGC
AGGTGCCGCCCCATCTTGCTCTGCGCGACGGTGACGATTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (50-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.857	90.439	0.568
pilU	Vibrio cholerae strain A1552	58.939	92.506	0.545
pilU	Acinetobacter baylyi ADP1	55.679	93.282	0.519
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.091	96.382	0.406
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.988	84.755	0.364
pilT	Vibrio cholerae strain A1552	42.988	84.755	0.364