Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ORD17_RS02645 Genome accession   NZ_CP118747
Coordinates   518838..519887 (-) Length   349 a.a.
NCBI ID   WP_374693399.1    Uniprot ID   -
Organism   Acidithiobacillus sp. AMEEHan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513838..524887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORD17_RS02620 - 514096..514770 (+) 675 WP_308389350.1 Crp/Fnr family transcriptional regulator -
  ORD17_RS02625 - 514897..515376 (+) 480 WP_308389351.1 hypothetical protein -
  ORD17_RS02630 - 515420..516562 (+) 1143 WP_308389352.1 FAD-dependent oxidoreductase -
  ORD17_RS02635 - 516538..517713 (-) 1176 WP_308389353.1 glycosyltransferase -
  ORD17_RS02640 pilU 517689..518825 (-) 1137 WP_308389354.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ORD17_RS02645 pilT 518838..519887 (-) 1050 WP_374693399.1 type IV pilus twitching motility protein PilT Machinery gene
  ORD17_RS02650 - 519976..520635 (+) 660 WP_308389356.1 YggS family pyridoxal phosphate-dependent enzyme -
  ORD17_RS02655 proC 520639..521460 (+) 822 WP_308389357.1 pyrroline-5-carboxylate reductase -
  ORD17_RS02660 - 521462..521731 (+) 270 WP_308389358.1 YggT family protein -
  ORD17_RS02665 bioB 521769..522707 (+) 939 WP_308389359.1 biotin synthase BioB -
  ORD17_RS02670 - 522701..523204 (-) 504 WP_308389360.1 acyloxyacyl hydrolase -
  ORD17_RS02675 - 523426..524439 (+) 1014 WP_308389361.1 HlyD family secretion protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38521.25 Da        Isoelectric Point: 6.7263

>NTDB_id=794958 ORD17_RS02645 WP_374693399.1 518838..519887(-) (pilT) [Acidithiobacillus sp. AMEEHan]
MDVTDLLGFAVKNNASDIHVSAGLPPMLRINGEIKPANVPPLSKEIVHAMIYDIMNDAQRKWYEENLEIDFAIDVPQVAR
FRINAFQQDRGPAAAFRTIPAKVLSLEELNAPPVFKEIIDVPRGLVLVTGPTGSGKSTTLAAMVNHINNHRQDHIITIED
PIEFLHTPNKCLINQREVGPNTKSFENALRSALREDPDVILVGELRDLETMRLALTAAETGHVVFGTLHTSSAPKTIDRI
IDSFPGGEKDMVRAMLSESLRAVIAQTLIPTADHRGRVAAHEIMIANPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LLGLVRAHKISRDDALRRAQNKESLANVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=794958 ORD17_RS02645 WP_374693399.1 518838..519887(-) (pilT) [Acidithiobacillus sp. AMEEHan]
ATGGATGTCACCGATCTGCTCGGTTTCGCGGTAAAAAACAATGCGTCGGATATCCACGTTTCCGCTGGCTTGCCGCCCAT
GCTGCGTATCAACGGCGAGATCAAGCCAGCCAACGTGCCACCTCTGAGCAAGGAGATCGTGCATGCCATGATCTACGACA
TCATGAACGATGCCCAGCGGAAATGGTACGAAGAGAATCTGGAGATCGATTTCGCCATCGATGTACCTCAGGTGGCGCGC
TTTCGTATCAATGCCTTTCAGCAGGATCGTGGTCCAGCAGCAGCGTTTCGCACCATCCCTGCCAAGGTCCTGAGTCTGGA
AGAGCTGAATGCGCCGCCGGTATTCAAGGAAATCATCGACGTGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACGGGAT
CGGGCAAATCGACCACCCTCGCGGCCATGGTGAACCACATCAACAACCATCGTCAGGACCATATCATTACCATCGAGGAC
CCGATCGAGTTTCTGCATACCCCCAATAAGTGTCTGATCAATCAGCGTGAGGTCGGGCCCAACACGAAATCCTTTGAAAA
CGCCCTGCGCTCCGCCCTGCGTGAGGATCCGGATGTGATTCTGGTAGGTGAGTTGCGTGATCTCGAAACCATGCGTCTGG
CTCTCACTGCGGCGGAGACCGGGCACGTGGTCTTTGGTACCCTGCACACCAGCTCTGCTCCGAAAACCATCGACCGAATC
ATCGATTCCTTCCCCGGCGGGGAGAAGGATATGGTGCGGGCCATGCTCTCCGAGTCCCTACGCGCGGTGATCGCGCAAAC
CCTCATCCCGACCGCCGACCATCGCGGGCGGGTAGCGGCGCACGAAATCATGATTGCCAACCCGGCGATCCGCAATCTGA
TCCGGGAAAACAAGGTGGCACAGATGTATTCCGTCATTCAGACAGGCCAGAATCAGGGGATGCAGACCCTCGACCAGTGT
TTACTCGGCTTGGTCCGGGCGCACAAGATCAGCCGCGACGATGCACTGCGTAGGGCGCAGAACAAAGAGAGCCTCGCCAA
CGTCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

67.536

98.854

0.668
  pilT Acinetobacter baumannii strain A118

67.536

98.854

0.668
  pilT Acinetobacter nosocomialis M2

67.536

98.854

0.668
  pilT Pseudomonas aeruginosa PAK

66.957

98.854

0.662
  pilT Acinetobacter baylyi ADP1

66.377

98.854

0.656
  pilT Pseudomonas stutzeri DSM 10701

65.797

98.854

0.65
  pilT Neisseria meningitidis 8013

64.74

99.14

0.642
  pilT Neisseria gonorrhoeae MS11

64.451

99.14

0.639
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.652

91.404

0.628
  pilT Vibrio cholerae strain A1552

68.652

91.404

0.628
  pilT Legionella pneumophila strain Lp02

64.035

97.994

0.628
  pilT Legionella pneumophila strain ERS1305867

64.035

97.994

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.429

100

0.516
  pilU Acinetobacter baylyi ADP1

43.642

99.14

0.433
  pilU Pseudomonas stutzeri DSM 10701

42.029

98.854

0.415
  pilU Vibrio cholerae strain A1552

40.29

98.854

0.398