Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ORD17_RS02640 Genome accession   NZ_CP118747
Coordinates   517689..518825 (-) Length   378 a.a.
NCBI ID   WP_308389354.1    Uniprot ID   -
Organism   Acidithiobacillus sp. AMEEHan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512689..523825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORD17_RS02610 purE 512827..513327 (-) 501 WP_374693398.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  ORD17_RS02615 trmB 513540..513884 (+) 345 Protein_507 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ORD17_RS02620 - 514096..514770 (+) 675 WP_308389350.1 Crp/Fnr family transcriptional regulator -
  ORD17_RS02625 - 514897..515376 (+) 480 WP_308389351.1 hypothetical protein -
  ORD17_RS02630 - 515420..516562 (+) 1143 WP_308389352.1 FAD-dependent oxidoreductase -
  ORD17_RS02635 - 516538..517713 (-) 1176 WP_308389353.1 glycosyltransferase -
  ORD17_RS02640 pilU 517689..518825 (-) 1137 WP_308389354.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ORD17_RS02645 pilT 518838..519887 (-) 1050 WP_374693399.1 type IV pilus twitching motility protein PilT Machinery gene
  ORD17_RS02650 - 519976..520635 (+) 660 WP_308389356.1 YggS family pyridoxal phosphate-dependent enzyme -
  ORD17_RS02655 proC 520639..521460 (+) 822 WP_308389357.1 pyrroline-5-carboxylate reductase -
  ORD17_RS02660 - 521462..521731 (+) 270 WP_308389358.1 YggT family protein -
  ORD17_RS02665 bioB 521769..522707 (+) 939 WP_308389359.1 biotin synthase BioB -
  ORD17_RS02670 - 522701..523204 (-) 504 WP_308389360.1 acyloxyacyl hydrolase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41942.48 Da        Isoelectric Point: 6.3176

>NTDB_id=794957 ORD17_RS02640 WP_308389354.1 517689..518825(-) (pilU) [Acidithiobacillus sp. AMEEHan]
MPVLDDLLRLMMERNGSDLYFTVGAPPVMKIDGRAVPVGDEPLRPAQSLALAKEILGLERLQDFQHEKEANLALSVPGVG
RFRVNGFFQRNEISFVIRAIKTQIPTLDQLHMPPILKELAMTSRGLVLFVGATGSGKSTSLASMLQHRNASSAGHILTIE
DPIEYLHKNIKSIVNQREVGIDTLNYENALENALREAPDVILIGEVRSRATMEHAVAYAETGHLCMSTLHANNANQAVER
IINFFPEERKRQLLMDLSLNLRAVVSQRLLPLKGRPGRIAAMEVLINTPTIADLIYRGEVGLLKDAMARPNDVGMQTFDQ
SLVKLFMEGMIEYEDAIRGADSQNDLRLAIKMECMKRGLEDPGSKVEEATQWRIGGNA

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=794957 ORD17_RS02640 WP_308389354.1 517689..518825(-) (pilU) [Acidithiobacillus sp. AMEEHan]
ATGCCAGTGCTTGATGACCTGTTACGGTTGATGATGGAAAGAAACGGTTCCGACCTGTATTTCACGGTGGGCGCGCCGCC
GGTGATGAAGATCGATGGGCGCGCCGTTCCCGTTGGTGATGAGCCCCTGAGGCCCGCCCAAAGTCTGGCCTTGGCGAAGG
AGATCCTGGGTTTGGAGCGTCTGCAGGATTTCCAGCACGAAAAGGAGGCCAATCTCGCCTTGTCCGTCCCCGGTGTGGGT
AGGTTTCGGGTCAACGGATTTTTTCAGCGAAACGAGATTTCCTTTGTTATCCGTGCCATCAAGACGCAGATCCCGACTCT
CGACCAGTTGCACATGCCGCCGATTCTGAAGGAACTGGCGATGACCTCCCGCGGCTTGGTACTGTTTGTGGGTGCTACCG
GCTCCGGAAAAAGCACCTCTTTGGCGTCCATGCTGCAACATCGTAATGCCAGTTCTGCAGGGCATATTCTGACCATTGAA
GATCCCATCGAGTATCTCCACAAAAATATCAAGTCGATCGTCAATCAGCGCGAAGTCGGCATTGATACCCTGAATTATGA
GAATGCCCTGGAAAATGCCCTGCGTGAGGCCCCCGACGTCATCCTGATCGGCGAAGTGCGAAGCCGCGCGACCATGGAGC
ATGCAGTGGCCTACGCGGAAACCGGCCACCTTTGTATGTCCACTTTGCATGCCAACAATGCCAATCAGGCGGTGGAGCGC
ATCATCAACTTTTTCCCGGAGGAGCGTAAGAGGCAGTTGCTCATGGACCTCTCCCTGAACCTCCGCGCCGTAGTTTCGCA
GCGTCTTCTCCCTCTCAAGGGCCGACCAGGACGAATTGCGGCAATGGAAGTCCTGATCAATACGCCGACGATCGCCGATC
TCATCTATCGCGGGGAAGTCGGCTTGTTGAAAGATGCCATGGCACGGCCCAACGATGTCGGTATGCAGACTTTCGATCAG
TCGCTGGTGAAGCTGTTTATGGAAGGCATGATCGAGTACGAGGATGCCATCCGTGGCGCCGATTCCCAGAATGATCTACG
CCTTGCGATCAAGATGGAGTGCATGAAGCGCGGCCTGGAGGACCCTGGTTCCAAGGTCGAGGAAGCGACCCAATGGCGTA
TCGGTGGCAATGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.707

97.354

0.542
  pilU Vibrio cholerae strain A1552

53.74

95.503

0.513
  pilU Acinetobacter baylyi ADP1

54

92.593

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.499

91.799

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

87.302

0.365
  pilT Vibrio cholerae strain A1552

41.818

87.302

0.365
  pilT Pseudomonas aeruginosa PAK

41.441

88.095

0.365