Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SKTS_RS02540 Genome accession   NZ_AP022853
Coordinates   510653..511696 (+) Length   347 a.a.
NCBI ID   WP_173059867.1    Uniprot ID   A0A6F8V7D5
Organism   Sulfurimicrobium lacus strain skT11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505653..516696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SKTS_RS02510 (SKTS_04880) - 505733..506332 (+) 600 WP_173059848.1 riboflavin synthase -
  SKTS_RS02515 (SKTS_04890) ribBA 506329..507420 (+) 1092 WP_173059851.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  SKTS_RS02520 (SKTS_04900) ribH 507431..507898 (+) 468 WP_173059854.1 6,7-dimethyl-8-ribityllumazine synthase -
  SKTS_RS02525 (SKTS_04910) nusB 507899..508333 (+) 435 WP_173059857.1 transcription antitermination factor NusB -
  SKTS_RS02530 (SKTS_04920) mgtE 508400..509842 (-) 1443 WP_173059861.1 magnesium transporter -
  SKTS_RS02535 (SKTS_04930) - 509901..510605 (-) 705 WP_173059864.1 YggS family pyridoxal phosphate-dependent enzyme -
  SKTS_RS02540 (SKTS_04940) pilT 510653..511696 (+) 1044 WP_173059867.1 type IV pilus twitching motility protein PilT Machinery gene
  SKTS_RS02545 (SKTS_04950) pilU 511833..512969 (+) 1137 WP_173059870.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SKTS_RS02550 (SKTS_04960) - 512987..513409 (+) 423 WP_173059873.1 CZB domain-containing protein -
  SKTS_RS02555 (SKTS_04970) - 513419..514582 (-) 1164 WP_173059876.1 THUMP domain-containing protein -
  SKTS_RS02560 (SKTS_04980) - 514712..516364 (+) 1653 WP_173059879.1 AMP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38222.83 Da        Isoelectric Point: 6.8315

>NTDB_id=79461 SKTS_RS02540 WP_173059867.1 510653..511696(+) (pilT) [Sulfurimicrobium lacus strain skT11]
MEISDLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDSLDHKGVHGMVYDIMNDGQRKFYEENLEVDFSFEIPGLAR
FRVNAYNQNRGAAAVFRTIPSKVLTLEQLNCPPIFKEIADQPRGIVLVTGPTGSGKSTTLAAMINHINENEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKEMVRSMLSESLRAVIAQTLLKTKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQC
LTDLVRRNVVAPNEARIKASNKDSFAG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=79461 SKTS_RS02540 WP_173059867.1 510653..511696(+) (pilT) [Sulfurimicrobium lacus strain skT11]
ATGGAAATCTCTGATCTACTCGCCTTCTCGGTCAAGAACAAGGCATCCGACCTGCACCTGTCCGCGGGTTTGCCGCCGAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAATGTGGACTCGCTGGACCACAAGGGCGTGCACGGCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAATTCTACGAAGAAAATCTGGAGGTGGACTTTTCCTTCGAGATCCCCGGCCTGGCCCGC
TTCCGCGTCAACGCATACAACCAGAACCGGGGCGCCGCAGCGGTATTCCGTACCATTCCTTCCAAGGTACTGACGCTCGA
ACAACTCAATTGCCCGCCCATTTTCAAGGAAATCGCCGACCAACCGCGCGGCATCGTGCTGGTGACCGGACCTACCGGAT
CGGGTAAATCGACCACCCTGGCGGCGATGATCAACCACATCAACGAGAACGAATACGGCCATATTCTCACCGTGGAAGAC
CCGATCGAGTTCGTGCATGAAAGCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGACCGCATACGCTTTCCTTCGCCAA
CGCGCTGCGTTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTTGAAACCATTCGCCTGG
CCCTGACCGCGGCGGAAACCGGTCACCTCGTGTTCGGTACCCTGCATACCAGTTCAGCAGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCAGCCGCCGAAAAGGAAATGGTCCGGTCCATGCTTTCCGAATCCCTGCGCGCGGTTATTGCGCAGAC
GCTGCTCAAGACCAAGGACGGCAGCGGCCGGGTCGCGGCGCACGAAATCATGATCGGCACGCCGGCTATCCGTAACCTGA
TCCGGGAAAACAAGATCGCGCAAATGTATTCCGCGATCCAGACCGGCCAGAATGTCGGCATGCAGACACTGGATCAGTGT
CTTACCGACCTGGTGCGGCGCAATGTGGTTGCCCCCAACGAGGCGCGTATCAAAGCGTCGAACAAGGACAGCTTCGCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F8V7D5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Pseudomonas aeruginosa PAK

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

71.965

99.712

0.718
  pilT Neisseria gonorrhoeae MS11

71.676

99.712

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.639

97.406

0.669
  pilT Vibrio cholerae strain A1552

68.639

97.406

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

99.712

0.504
  pilU Pseudomonas stutzeri DSM 10701

40.87

99.424

0.406
  pilU Acinetobacter baylyi ADP1

43.077

93.66

0.403
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395

Multiple sequence alignment