Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SKTS_RS02545 Genome accession   NZ_AP022853
Coordinates   511833..512969 (+) Length   378 a.a.
NCBI ID   WP_173059870.1    Uniprot ID   -
Organism   Sulfurimicrobium lacus strain skT11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506833..517969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SKTS_RS02520 (SKTS_04900) ribH 507431..507898 (+) 468 WP_173059854.1 6,7-dimethyl-8-ribityllumazine synthase -
  SKTS_RS02525 (SKTS_04910) nusB 507899..508333 (+) 435 WP_173059857.1 transcription antitermination factor NusB -
  SKTS_RS02530 (SKTS_04920) mgtE 508400..509842 (-) 1443 WP_173059861.1 magnesium transporter -
  SKTS_RS02535 (SKTS_04930) - 509901..510605 (-) 705 WP_173059864.1 YggS family pyridoxal phosphate-dependent enzyme -
  SKTS_RS02540 (SKTS_04940) pilT 510653..511696 (+) 1044 WP_173059867.1 type IV pilus twitching motility protein PilT Machinery gene
  SKTS_RS02545 (SKTS_04950) pilU 511833..512969 (+) 1137 WP_173059870.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SKTS_RS02550 (SKTS_04960) - 512987..513409 (+) 423 WP_173059873.1 CZB domain-containing protein -
  SKTS_RS02555 (SKTS_04970) - 513419..514582 (-) 1164 WP_173059876.1 THUMP domain-containing protein -
  SKTS_RS02560 (SKTS_04980) - 514712..516364 (+) 1653 WP_173059879.1 AMP-binding protein -
  SKTS_RS02565 (SKTS_04990) - 516361..516771 (-) 411 WP_173059882.1 CopD family protein -
  SKTS_RS02570 (SKTS_05000) - 516861..517403 (+) 543 WP_173059885.1 TlpA disulfide reductase family protein -
  SKTS_RS02575 (SKTS_05010) - 517430..517969 (-) 540 WP_173059888.1 DinB family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42147.51 Da        Isoelectric Point: 6.3497

>NTDB_id=79462 SKTS_RS02545 WP_173059870.1 511833..512969(+) (pilU) [Sulfurimicrobium lacus strain skT11]
MEREQAQKFMHDLLRLMISKKGSDLFITAGFPPAIKVDGKVTPVSNQTLTPAHSQELAMAIMNSKQASEFEATKECNFAI
SPPDIGRFRVNAFVQQSRVGLVLRTITAKIPKFEDLGLPEVLKDVAMTKRGLVVFVGGTGSGKSTSLAAMVGYRNENSFG
HIITIEDPVEYVHDHKNCIVTQRDVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLSTLHANST
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLIPMKEGKGRAAAIEIMLNSPLISDLIFKGEVHEIKEIISKSRELG
MQTFDQSLFDLYEAGMISYEDALRNADSVNDLRLKIKLHGQESKDKDLMSGIDHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=79462 SKTS_RS02545 WP_173059870.1 511833..512969(+) (pilU) [Sulfurimicrobium lacus strain skT11]
ATGGAACGCGAACAAGCTCAGAAATTCATGCATGATCTGCTGCGGCTGATGATCAGCAAGAAAGGGTCGGACCTGTTCAT
TACCGCCGGCTTCCCGCCCGCCATCAAGGTGGATGGCAAGGTTACCCCGGTTTCCAATCAGACCCTTACGCCAGCCCATT
CACAGGAACTGGCGATGGCGATCATGAACAGCAAGCAGGCGTCGGAGTTCGAAGCCACCAAGGAATGCAATTTCGCCATC
AGCCCGCCGGATATCGGCCGTTTCCGCGTCAATGCCTTCGTCCAGCAGTCACGCGTCGGTCTGGTGCTGCGCACCATTAC
CGCCAAAATTCCCAAATTCGAAGACCTGGGCCTGCCGGAGGTGCTCAAGGACGTGGCCATGACCAAGCGCGGCCTGGTGG
TATTCGTCGGCGGCACCGGTTCTGGCAAATCCACCTCGCTGGCGGCGATGGTCGGTTACCGCAACGAAAACAGCTTCGGC
CACATCATTACCATCGAAGACCCGGTGGAGTACGTGCACGATCACAAGAATTGCATCGTCACCCAGCGTGACGTCGGGGT
GGACACGGAGAACTGGTTCGCGGCGCTGAAAAACACGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCG
ACCGCGAAACCATGGATTACGCCATCGCCTTTGCCGAAACCGGTCACTTGTGTCTCTCCACGCTGCATGCCAACAGCACC
AACCAGGCGCTGGACAGGATCATCAACTTCTTCCCCGAGGAGCGGCGCCAGCAACTGCTCATGGACCTTTCGCTTAACCT
CAAGGCTTTCGTCTCGCAGCGCCTGATTCCCATGAAGGAAGGCAAGGGGCGGGCGGCAGCGATCGAGATCATGCTCAACT
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGCACGAGATTAAGGAAATCATATCCAAGTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGATCTGTATGAGGCCGGCATGATCTCCTATGAGGATGCGTTGCGCAACGCCGA
TTCGGTCAACGATCTGCGCCTCAAGATCAAGTTGCACGGTCAGGAATCCAAGGACAAGGATCTGATGTCCGGAATCGATC
ACCTCGGGATCGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.246

94.709

0.608
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

56.305

90.212

0.508
  pilT Pseudomonas aeruginosa PAK

44.478

88.624

0.394
  pilT Acinetobacter nosocomialis M2

44.109

87.566

0.386
  pilT Acinetobacter baumannii strain A118

44.109

87.566

0.386
  pilT Acinetobacter baumannii D1279779

44.109

87.566

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.284

88.624

0.384
  pilT Acinetobacter baylyi ADP1

42.73

89.153

0.381
  pilT Legionella pneumophila strain Lp02

42.9

87.566

0.376
  pilT Legionella pneumophila strain ERS1305867

42.9

87.566

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.791

88.624

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

87.302

0.365
  pilT Vibrio cholerae strain A1552

41.818

87.302

0.365

Multiple sequence alignment